Stability of cross-linked dentin and its clinical application

描述（由申请人提供）：本提案的长期目标是为安娜·卡丽娜·贝德兰-鲁索博士（DOS，MS，PhD）提供机会，使其发展成为一名准备充分，具有竞争力和独立性的年轻科学家，将联合收割机与教学和实践相结合。申请人的最终目标是通过促进转化研究来改善患者护理。在K 08指导临床医生科学家奖期间获得的组织工程，口腔生物学和生物材料方面的额外培训将为Bedran- Russo博士快速过渡到独立临床医生科学家的支持性多学科环境中的牙科学术职业轨道提供必要的技能。她的研究和培训将在詹姆斯·德拉蒙德博士和安妮·乔治博士的指导下进行。申请人将在这项研究的基础上寻求额外的资金。该补助金的研究部分旨在调查胶原蛋白的生化修饰对牙本质性质的贡献，从而对粘合剂修复牙科治疗的耐久性的贡献。复合树脂修复后牙平均持续7年，此时界面失效是更换的主要原因，占所有更换的30-60%。纤维状I型胶原蛋白的完整性在与牙本质结合时是一个重要的问题，因为它是修复过程中涉及的主要牙齿组分。待测试的假设是牙本质中人工形成的胶原交联将导致修复界面具有改善的机械性能，以承受随时间推移的降解。具体目标如下：目标1。测定不同交联剂处理后牙本质的生物化学性质。将在短期和长期评价中评估健康和龋齿影响的人牙本质的生化特性（胶原交联分析- HPLC和免疫组织化学分析）。目的2：在短期和长期评价中评估经处理牙本质（完好和受龋齿影响）的机械性能。将使用原子力显微镜评价特定部位的刚度和硬度。使用万能试验机评估牙本质的极限强度和牙本质/修复体界面的粘结强度。这些生物化学和机械方法的结合将为牙本质胶原蛋白的质量和牙本质的长期耐久性提供相关的见解。

DESCRIPTION (provided by applicant): The long term goal of this proposal is to provide Dr. Ana Karina Bedran-Russo, DOS, MS, PhD with the opportunity to develop into a well prepared, competitive and independent young scientist that will combine an active research career with teaching and practice. The applicant's ultimate goal is to improve patient care by contributing to translational research. Additional training in tissue engineering, oral biology and biomaterials obtained during this K08 Mentored Clinician Scientist Award will provide the necessary skills for a dental academic career track within a supportive multidisciplinary environment for the rapid transition of Dr. Bedran- Russo to an independent clinician scientist. Her research and training will be conducted under the guidance of Dr. James Drummond and Dr. Anne George. The applicant will build upon this research to seek additional funding. The research component of this grant aims to investigate the contribution of biochemical modifications to collagen on the properties of dentin and consequently on the durability of adhesive restorative dental treatments. A composite resin restoration last an average of 7 years in posterior teeth at which time failure at the interface is the primary reason given for replacement and accounts for 30-60% of all replaced restorations. The integrity of fibrillar type I collagen is an important issue when bonding to dentin since it is the main tooth component involved during restorative procedures. The hypothesis to be tested is that the artificially formed collagen cross-links in dentin will result in restoration interfaces with improved mechanical properties to withstand degradation over time. The specific aims proposed are: Aim 1. Determine the biochemical properties of dentin following treatment with different crosslinking agents. Biochemical properties (collagen cross-linking analysis - HPLC and immunohistochemical analysis) will be assessed in sound and caries-affected human dentin in both short and long term evaluations. Aim 2: Assess the mechanical properties of the treated dentin (sound and caries-affected) at short-term and long-term evaluations. Stiffness and hardness will be evaluated in specific sites using an atomic force microscope. Ultimate strength of dentin and bond strength of the dentin/restoration interface will be assessed using a universal testing machine. These combined biochemical and mechanical approaches will provide insights to correlate quality of dentin collagen and the long-term durability of restorations.