Stability of cross-linked dentin and its clinical application

描述（由申请人提供）：本提案的长期目标是为Ana Karina Bedran-Russo博士，DOS， MS， PhD提供发展成为一个准备充分，有竞争力和独立的年轻科学家的机会，将积极的研究事业与教学和实践相结合。申请人的最终目标是通过促进转化研究来改善患者护理。在K08指导临床医生科学家奖期间获得的组织工程，口腔生物学和生物材料方面的额外培训将为Bedran- Russo博士在一个支持性的多学科环境中快速过渡到独立临床医生科学家的牙科学术生涯轨道提供必要的技能。她的研究和培训将在Dr. James Drummond和Dr. Anne George的指导下进行。申请人将在此研究基础上寻求额外资助。这项资助的研究部分旨在研究胶原蛋白的生化修饰对牙本质特性的影响，从而对牙釉质修复治疗的耐久性产生影响。复合树脂修复体在后牙的平均使用寿命为7年，其中界面失效是更换的主要原因，占所有更换修复体的30-60%。纤维状I型胶原蛋白是修复过程中涉及的主要牙齿成分，因此在与牙本质结合时，其完整性是一个重要问题。需要验证的假设是，人工形成的牙本质胶原交联将导致修复界面具有改善的机械性能，以承受随着时间的推移降解。提出的具体目标是：目标1。测定不同交联剂处理后牙本质的生化特性。生物化学特性（胶原交联分析-高效液相色谱和免疫组织化学分析）将在短期和长期评估中评估健全和龋齿影响的人类牙本质。目的2：在短期和长期评估中评估治疗后牙本质（健康和龋齿）的力学特性。将使用原子力显微镜评估特定部位的刚度和硬度。牙本质的极限强度和牙本质/修复体界面的结合强度将使用通用试验机进行评估。这些结合了生物化学和力学的方法将为牙本质胶原蛋白的质量和修复体的长期耐久性提供见解。

DESCRIPTION (provided by applicant): The long term goal of this proposal is to provide Dr. Ana Karina Bedran-Russo, DOS, MS, PhD with the opportunity to develop into a well prepared, competitive and independent young scientist that will combine an active research career with teaching and practice. The applicant's ultimate goal is to improve patient care by contributing to translational research. Additional training in tissue engineering, oral biology and biomaterials obtained during this K08 Mentored Clinician Scientist Award will provide the necessary skills for a dental academic career track within a supportive multidisciplinary environment for the rapid transition of Dr. Bedran- Russo to an independent clinician scientist. Her research and training will be conducted under the guidance of Dr. James Drummond and Dr. Anne George. The applicant will build upon this research to seek additional funding. The research component of this grant aims to investigate the contribution of biochemical modifications to collagen on the properties of dentin and consequently on the durability of adhesive restorative dental treatments. A composite resin restoration last an average of 7 years in posterior teeth at which time failure at the interface is the primary reason given for replacement and accounts for 30-60% of all replaced restorations. The integrity of fibrillar type I collagen is an important issue when bonding to dentin since it is the main tooth component involved during restorative procedures. The hypothesis to be tested is that the artificially formed collagen cross-links in dentin will result in restoration interfaces with improved mechanical properties to withstand degradation over time. The specific aims proposed are: Aim 1. Determine the biochemical properties of dentin following treatment with different crosslinking agents. Biochemical properties (collagen cross-linking analysis - HPLC and immunohistochemical analysis) will be assessed in sound and caries-affected human dentin in both short and long term evaluations. Aim 2: Assess the mechanical properties of the treated dentin (sound and caries-affected) at short-term and long-term evaluations. Stiffness and hardness will be evaluated in specific sites using an atomic force microscope. Ultimate strength of dentin and bond strength of the dentin/restoration interface will be assessed using a universal testing machine. These combined biochemical and mechanical approaches will provide insights to correlate quality of dentin collagen and the long-term durability of restorations.