ANTIBODY PRODUCTION AND MOUSE GENOTYPING CORE

抗体生产和小鼠基因分型核心

• 批准号: 7960500
• 负责人: SIDNEY Waldo WHITEHEART
• 金额: $ 4.75万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960500
• 关键词: Advertisements; Animals; Antibody Formation; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; DNA; Electrophoresis; Equipment; Floor; Funding; Genotype; Grant; Heating; Institution; Instruction; Interview; Methodology; Molecular; Mus; Preparation; Protocols documentation; Reaction; Research; Research Personnel; Resources; Running; Sampling; Sepharose; Services; Source; Technology; Testing; United States National Institutes of Health; Writing; base; human disease

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genotyping core will be based on the second floor of the BBSRB. At present the core is still being set up. We have developed the PCR-based methodology for genotype analysis and have optimized the DNA preparation protocols. All of the technologies have been tested on a small scale (~50 animals). The equipment, heat blocks, Robocycler PCR machine, repeating pipette sets, and large format agarose electrophoresis apparati, are in place. A detailed set of user instructions are being written to describe what services are to be provided and what the user needs to supply the core in terms of sample, PCR primer sets, and reaction conditions. An advertisement for a technician to run the facility has been circulated and applicants are being interviewed. The technician will handle the mouse genotyping and antibody production.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 基因分型核心将位于BBSRB的二楼。 目前，核心仍在建立中。 我们已经开发了基于PCR的基因型分析方法，并优化了DNA制备方案。 所有这些技术都在小规模（约50只动物）上进行了测试。 设备、加热块、Robocycler PCR机、重复移液器组和大型琼脂糖电泳仪均已就位。 正在编写一套详细的用户说明，以描述将提供哪些服务，以及用户需要在样品、PCR引物组和反应条件方面提供哪些核心。 已经散发了一份招聘技术人员管理该设施的广告，正在对申请人进行面试。 技术人员将处理小鼠基因分型和抗体生产。