IMAGING CORE

成像核心

• 批准号: 8168246
• 负责人: SIDNEY Waldo WHITEHEART
• 金额: $ 1.49万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168246
• 关键词: Cells; Cellular Structures; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Core Facility; Data Analyses; Data Storage and Retrieval; Funding; Grant; Housing; Image; Imaging Techniques; Individual; Institution; Internet; Life; Microscope; Preparation; Publications; Research; Research Personnel; Resolution; Resources; Sampling; Slide; Source; Spottings; Staging; Techniques; United States National Institutes of Health; cellular imaging; digital; digital imaging; flexibility; image processing; instrument; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The imaging core facility houses three epifluorescence microscopes (Nikon Eclipse 600, Zeiss Axiovert 100 and 200M) with digital image capture capability (Spot CE and Orca ER digital cameras with Metaview and Open Lab software). The Eclipse 600 will allow the high resolution imaging of cells and sub-cellular compartments. This instrument can be used for detailed analysis of various cellular structures. The core also contains two inverted epifluorescence microscopes. The Axiovert 200M is equipped with stage controllers to allow long term analysis of live cells. The Axiovert 100 can be used for analysis of cells in tissue culture dishes or fixed samples on slides. The flexibility of the instruments in the facility will enable the core users a wide range of analytical techniques with which to pursue their individual projects. The facility contains two computers for image processing. The computers have complete internet access and network access to color printers and to data storage. The core director, Dr. Whiteheart, will be available to offer advice on sample preparation, on data analysis, and on imaging procedures. In addition, Dr. Whiteheart will also advise in the preparation of figures and selection of images for publication.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Imaging Core设施具有三个具有数字图像捕获能力（带有Metaview和Open Lab Software）的数字图像捕获能力（斑点CE和Orca ER数字摄像头），包含三个Epifluorecence显微镜（Nikon Eclipse 600，Zeiss Axiovert 100和200m）。 Eclipse 600将允许对细胞和亚细胞隔室进行高分辨率成像。该仪器可用于详细分析各种细胞结构。该核心还包含两个倒倒荧光显微镜。 AxioVert 200M配备了舞台控制器，可以长期分析活细胞。 Axiovert 100可用于分析组织培养皿中的细胞或幻灯片上的固定样品。该工具在设施中的灵活性将使核心用户能够采用广泛的分析技术来追求其各个项目。该设施包含两台用于图像处理的计算机。计算机具有完整的Internet访问和网络访问颜色打印机和数据存储。 WhiteHeart博士的核心主管将提供有关样本准备，数据分析和成像程序的建议。此外，WhiteHeart博士还将在准备数字和出版图像的选择时提供建议。