IMAGING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 成像核心设施包括三台具有数字图像捕获能力的荧光显微镜(尼康Eclipse 600、蔡司Axiovert 100和200M)(带有Metaview和Open Lab软件的SPOT CE和Orca ER数码相机)。日食600将允许对细胞和亚细胞隔间进行高分辨率成像。该仪器可用于各种细胞结构的详细分析。核心还包括两个倒置的荧光显微镜。Axiovert 200M配备了阶段控制器，可以对活细胞进行长期分析。Axiovert 100可用于分析组织培养皿中的细胞或玻片上的固定样品。该设施中仪器的灵活性将使核心用户能够使用广泛的分析技术来开展其个别项目。该设施包括两台用于图像处理的计算机。这些计算机有完全的互联网接入和彩色打印机和数据存储的网络接入。核心主任怀特哈特博士将就样品制备、数据分析和成像程序提供建议。此外，白心博士还将为准备数字和选择要出版的图像提供建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The imaging core facility houses three epifluorescence microscopes (Nikon Eclipse 600, Zeiss Axiovert 100 and 200M) with digital image capture capability (Spot CE and Orca ER digital cameras with Metaview and Open Lab software). The Eclipse 600 will allow the high resolution imaging of cells and sub-cellular compartments. This instrument can be used for detailed analysis of various cellular structures. The core also contains two inverted epifluorescence microscopes. The Axiovert 200M is equipped with stage controllers to allow long term analysis of live cells. The Axiovert 100 can be used for analysis of cells in tissue culture dishes or fixed samples on slides. The flexibility of the instruments in the facility will enable the core users a wide range of analytical techniques with which to pursue their individual projects. The facility contains two computers for image processing. The computers have complete internet access and network access to color printers and to data storage. The core director, Dr. Whiteheart, will be available to offer advice on sample preparation, on data analysis, and on imaging procedures. In addition, Dr. Whiteheart will also advise in the preparation of figures and selection of images for publication.