IMAGING CORE

成像核心

• 批准号: 8360572
• 负责人: SIDNEY Waldo WHITEHEART
• 金额: $ 1.47万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360572
• 关键词: Cells; Cellular Structures; Centers of Research Excellence; Color; Computer software; Computers; Core Facility; Data Analyses; Data Storage and Retrieval; Funding; Grant; Housing; Image; Imaging Techniques; Individual; Internet; Life; Microscope; Molecular; National Center for Research Resources; Preparation; Principal Investigator; Publications; Research; Research Infrastructure; Resolution; Resources; Sampling; Slide; Source; Spottings; Staging; Techniques; United States National Institutes of Health; base; cellular imaging; cost; digital; digital imaging; flexibility; human disease; image processing; instrument; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The imaging core facility houses three epifluorescence microscopes (Nikon Eclipse 600, Zeiss Axiovert 100 and 200M) with digital image capture capability (Spot CE and Orca ER digital cameras with Metaview and Open Lab software). The Eclipse 600 will allow the high resolution imaging of cells and sub-cellular compartments. This instrument can be used for detailed analysis of various cellular structures. The core also contains two inverted epifluorescence microscopes. The Axiovert 200M is equipped with stage controllers to allow long term analysis of live cells. The Axiovert 100 can be used for analysis of cells in tissue culture dishes or fixed samples on slides. The flexibility of the instruments in the facility will enable the core users a wide range of analytical techniques with which to pursue their individual projects. The facility contains two computers for image processing. The computers have complete internet access and network access to color printers and to data storage. The core director, Dr. Whiteheart, will be available to offer advice on sample preparation, on data analysis, and on imaging procedures. In addition, Dr. Whiteheart will also advise in the preparation of figures and selection of images for publication.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 成像核心设施包括三台具有数字图像捕获能力的荧光显微镜(尼康Eclipse 600、蔡司Axiovert 100和200M)(带有Metaview和Open Lab软件的SPOT CE和Orca ER数码相机)。日食600将允许对细胞和亚细胞隔间进行高分辨率成像。该仪器可用于各种细胞结构的详细分析。核心还包括两个倒置的荧光显微镜。Axiovert 200M配备了阶段控制器，可以对活细胞进行长期分析。Axiovert 100可用于分析组织培养皿中的细胞或玻片上的固定样品。该设施中仪器的灵活性将使核心用户能够使用广泛的分析技术来开展其个别项目。该设施包括两台用于图像处理的计算机。这些计算机有完全的互联网接入和彩色打印机和数据存储的网络接入。核心主任怀特哈特博士将就样品制备、数据分析和成像程序提供建议。此外，白心博士还将为准备数字和选择要出版的图像提供建议。