CHARACTERIZATION OF MOB1 DYNAMICS IN LIVING CELLS

活细胞中 MOB1 动力学的表征

基本信息

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Maintenance of chromosomal ploidy during cell division requires a precise coordination of chromosome segregation and cytoplasmic partitioning (cytokinesis) such that the contractile ring does not assemble before the onset of anaphase. Progression of dividing cells into anaphase into cytokinesis is dependent on the spindle assembly checkpoint, which monitors chromosome attachment to the spindle and regulates the onset of sister chromatid segregation and CDK1 inactivation (mitotic exit). In yeast, the mitotic checkpoint also regulates a signaling cascade termed the Septation Initiation Network (SIN) that regulates the initiation of cytokinesis. Few functional homologues of the SIN have been identified in higher eukaryotes, but a terminal component named Mob1 has been identified in all eukaryotes. Expression of GFP chimeras of the four human Mob1s reveal that Mob1 localizes to the spindle poles and kinetochores up until anaphase onset, and the central spindle and midbody during cytokinesis. Additionally, we have identified a novel interaction between Mob1A and LATS2 kinase, which may serve as the functional homolog of the yeast Dbf2/Sid2 kinase in mammalian cells. Using these preliminary studies as a foundation, this application seeks to evaluate the roles that Mob1 plays in coordinating mitosis and cytokinesis in mammalian cells. The lines of experimentation proposed in this application will characterize the localization dynamics of the four human Mob1 family members using co-localization studies and live cell imaging. Further, the role of Mob1 in the coordination of mitosis and cytokinesis will be assessed by specifically disrupting Mob1/Lats2 interactions in vivo by siRNA knockdown of Mob1 or by expression of Lats2-binding mutants of Mob1. These efforts should ultimately lead to a clearer understanding of how mitosis and cytokinesis is coordinated, and confirm whether the strategies employed by unicellular fungi to regulate septation are conserved in higher eukaryotes.
这个子项目是众多研究子项目之一

项目成果

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Charles Bradley Shuster其他文献

Charles Bradley Shuster的其他文献

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{{ truncateString('Charles Bradley Shuster', 18)}}的其他基金

Parameters that determine cell fate during mitotic arrest
有丝分裂停滞期间决定细胞命运的参数
  • 批准号:
    10617385
  • 财政年份:
    2022
  • 资助金额:
    $ 12.87万
  • 项目类别:
Parameters that determine cell fate during mitotic arrest
有丝分裂停滞期间决定细胞命运的参数
  • 批准号:
    10409136
  • 财政年份:
    2022
  • 资助金额:
    $ 12.87万
  • 项目类别:
Parameters that determine cell fate during mitotic arrest
有丝分裂停滞期间决定细胞命运的参数
  • 批准号:
    10797794
  • 财政年份:
    2022
  • 资助金额:
    $ 12.87万
  • 项目类别:
Spindle orientation along the developmental axes in echinoderm embryos
棘皮动物胚胎沿发育轴的纺锤体方向
  • 批准号:
    8733008
  • 财政年份:
    2014
  • 资助金额:
    $ 12.87万
  • 项目类别:
DEVELOPMENT OF NOVEL SMALL MOLECULE INHIBITORS OF AURORA B KINASE SIGNALING
新型 AURORA B 激酶信号传导小分子抑制剂的开发
  • 批准号:
    8359753
  • 财政年份:
    2011
  • 资助金额:
    $ 12.87万
  • 项目类别:
DEVELOPMENT OF NOVEL SMALL MOLECULE INHIBITORS OF AURORA B KINASE SIGNALING
新型 AURORA B 激酶信号传导小分子抑制剂的开发
  • 批准号:
    8167576
  • 财政年份:
    2010
  • 资助金额:
    $ 12.87万
  • 项目类别:
Mob1 Localization and Function During Mitosis
Mob1 在有丝分裂期间的定位和功能
  • 批准号:
    8292148
  • 财政年份:
    2009
  • 资助金额:
    $ 12.87万
  • 项目类别:
Mob1 Localization and Function During Mitosis
Mob1 在有丝分裂期间的定位和功能
  • 批准号:
    7904769
  • 财政年份:
    2009
  • 资助金额:
    $ 12.87万
  • 项目类别:
Mob1 Localization and Function During Mitosis
Mob1 在有丝分裂期间的定位和功能
  • 批准号:
    8070024
  • 财政年份:
    2009
  • 资助金额:
    $ 12.87万
  • 项目类别:
Mob1 Localization and Function During Mitosis
Mob1 在有丝分裂期间的定位和功能
  • 批准号:
    7628920
  • 财政年份:
    2009
  • 资助金额:
    $ 12.87万
  • 项目类别:

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