Flow Cytometry

Wistar研究所的流式细胞仪设备提供流式细胞仪服务，并支持使用FLOW 由Wistar癌症中心研究人员提供的细胞学技术。该基金的目标是：1)提供 高质量、单参数和多参数分析和/或多种细胞分选的技术能力 来自同质或混合细胞群体的各种生物细胞；2)提供培训和专门知识，以 协助研究人员选择实验条件和试剂，以优化设备的使用 为他们的实验需要提供仪器；3)为流量分析提供建议和技术支持 用于出版、提交和纳入赠款申请的细胞学/细胞分选数据，以及 存储、存档和检索流式细胞仪数据。该实验室配备了一台达科细胞分离MoFlo高速 细胞分选机，Becton-Dickinson FACSCalibur流式细胞术系统，Cyan-ADP超 高速9色分析细胞仪、Coulter XL-MCL全自动分析细胞仪和Becton- 迪金森FACScan台式分析仪。2006年7月，研究所购买并投入使用一台 Becton-Dickinson LSR II分析细胞仪(7色分析)，于2007年11月升级 带有额外的激光器和探测器，可以进行10色分析。所有用户均可24小时访问 调查员操作的仪器。贝克曼-库尔特史诗精英电除尘器位于BSL3设施中， 允许对感染艾滋病毒的样本进行分类和分析。在主要设施中还包括其他 工作站、流式细胞仪期刊、协议指南和其他书面资源的图书馆。其他内容 设备包括荧光显微镜、4C冰箱和大量备件、用品和 工具，以便将仪器维持在最佳运行水平。自2003年以来，28例癌症患者 来自所有Wistar研究项目的中心研究小组已经利用了该设施，产生了 大约170种出版物。

The Wistar Institute Flow Cytometry Facility provides flow cytometric services and supports the use of flow cytometric techniques by Wistar Cancer Center investigators. The Facility's aims are to:1) provide the technological capability for high quality, single and multi-parameter analyses and/or cell sorting of many types of biological cells from homogeneous or mixed cell populations;2) provide training and expertise to assist investigators in choosing experimental conditions and reagents that optimize the use of the facility's instrumentation for their experimental needs;3) advise and provide technical support for analysis of flow cytometry/cell sorting data for publication, presentation, and inclusion in grant applications, along with storing, archiving, and retrieving flow cytometric data. The laboratory houses a DakoCytomation MoFlo highspeed cell sorter, a Becton-Dickinson FACSCalibur flow cytometry system, a Cytomation CYAN-ADP Ultra- High speed 9-color analytical cytometer, a Coulter XL-MCL automated analytical cytometer, and a Becton- Dickinson FACScan Bench top Analyzer. In July 2006, the Institute purchased and placed into service a Becton-Dickinson LSR II analytical cytometer (7-color analysis), which was upgraded in November 2007 with an additional laser and detectors allowing for 10-color analysis. 24-hour access is available for all of the investigator-operated instruments. A Beckman-Coulter EPICS Elite ESP is located in the BSL3 facility, allowing for both sorting and analysis of HIV-infected samples. Also in the main facility are additional workstations, a library of flow cytometry journals, protocol guides, and other written resources. Additional equipment includes fluorescence microscopes, 4¿C refrigerators, and extensive spare parts, supplies, and tools in order to maintain the instruments at optimal operating levels. Since 2003, twenty-eight Cancer Center research groups from all Wistar research programs have utilized the Facility, generating approximately 170 publications.