Flow Cytometry

Wistar研究所流式细胞仪设施提供流式细胞仪服务，并支持使用流式细胞仪。 Wistar癌症中心研究人员的细胞计数技术。该基金的目标是：1）提供 高质量、单参数和多参数分析和/或细胞分选的技术能力 来自同质或混合细胞群的生物细胞类型;2）提供培训和专业知识， 协助研究人员选择实验条件和试剂，优化设施的使用， 3）为流动分析提供建议和技术支持 细胞计数/细胞分选数据，用于出版、演示和纳入资助申请，沿着 存储、存档和检索流式细胞术数据。该实验室设有DakoCytomation MoFlo高速 细胞分选仪，Becton-Dickinson FACSCalibur流式细胞仪系统，Cytomation CYAN-ADP Ultra- 高速9色分析细胞仪、Coulter XL-MCL自动分析细胞仪和Becton- Dickinson FACScan台式分析仪。2006年7月，研究所购买了一辆 2007年11月升级的Becton-Dickinson LSR II分析型细胞仪（7色分析） 具有允许10色分析的附加激光器和检测器。24-小时访问可用于所有的 微波炉操作的仪器。Beckman-Coulter EPICS Elite ESP位于BSL 3设施中， 允许对HIV感染的样品进行分选和分析。此外，在主要设施是额外的 工作站、流式细胞术期刊库、协议指南和其他书面资源。额外 设备包括荧光显微镜，4 º C冰箱，和广泛的备件，用品， 工具，以保持仪器处于最佳运行水平。自2003年以来，28个癌症 来自所有Wistar研究计划的中心研究小组都利用了该设施， 约170篇文章

The Wistar Institute Flow Cytometry Facility provides flow cytometric services and supports the use of flow cytometric techniques by Wistar Cancer Center investigators. The Facility's aims are to:1) provide the technological capability for high quality, single and multi-parameter analyses and/or cell sorting of many types of biological cells from homogeneous or mixed cell populations;2) provide training and expertise to assist investigators in choosing experimental conditions and reagents that optimize the use of the facility's instrumentation for their experimental needs;3) advise and provide technical support for analysis of flow cytometry/cell sorting data for publication, presentation, and inclusion in grant applications, along with storing, archiving, and retrieving flow cytometric data. The laboratory houses a DakoCytomation MoFlo highspeed cell sorter, a Becton-Dickinson FACSCalibur flow cytometry system, a Cytomation CYAN-ADP Ultra- High speed 9-color analytical cytometer, a Coulter XL-MCL automated analytical cytometer, and a Becton- Dickinson FACScan Bench top Analyzer. In July 2006, the Institute purchased and placed into service a Becton-Dickinson LSR II analytical cytometer (7-color analysis), which was upgraded in November 2007 with an additional laser and detectors allowing for 10-color analysis. 24-hour access is available for all of the investigator-operated instruments. A Beckman-Coulter EPICS Elite ESP is located in the BSL3 facility, allowing for both sorting and analysis of HIV-infected samples. Also in the main facility are additional workstations, a library of flow cytometry journals, protocol guides, and other written resources. Additional equipment includes fluorescence microscopes, 4¿C refrigerators, and extensive spare parts, supplies, and tools in order to maintain the instruments at optimal operating levels. Since 2003, twenty-eight Cancer Center research groups from all Wistar research programs have utilized the Facility, generating approximately 170 publications.