Purchase of MVE Fusion Self-Sustaining Cryogenic Freezers

项目摘要 本申请的目标是安装两台创新的Chart MVE Fusion 1500自持式低温冷冻机 通过创建共享的新低温冷冻器场来实现-150摄氏度到-190摄氏度的长期存储，该场将扩展 该研究所的冷冻存储能力。新的低温加料器农场将支持35个活跃的研究实验室和核心 设施以及具有知识产权价值的研究样本的储存。因为额外的冰柜不能 由于空间限制而添加到当前农场设施，因此不能尝试进行翻新 现有农场的足迹，创建一个新的冷冻冷冻场对Wistar研究人员扩大他们的 目前用于支持92个NIH资助的项目，并追求新的研究和发现方向。二 尖端的、独立的、自给自足的冷冻机将创建一个新的、增加的冷冻机农场 在研究所的塔式地下室内(B02室)，不需要管道提供外源 更换液氮。值得注意的是，Chart MVE Fusion自给式冷冻机采用了创新的QDrive 低温冷却技术，仅对50升液氮运行，并在初始液体后完全依靠电力运行 氮气填充，因此不需要额外更换液氮。Chart MVE Fusion 1500低温 冷冻机是世界上第一台使用专利QDrive冷冻机技术的冷冻机，可以从冷冻机和冷冻机中排出热量 重复使用现有供应的液氮以保持冰柜温度。相比之下，传统的汽相 冷冻冷藏室需要80升液氮进行初始填充，然后每天例行填充以补偿 用于因使用和蒸发而造成的液氮损失。所要求的MVE Fusion 1500冷冻器将解决 同时满足两项科学需求：(A)扩大冷冻储存能力，增加72个储存 机架，以跟上研究所增长的步伐，以及(B)在新地点建立Cyofreezer装置，而不需要 在研究所向自给自足的绿色技术过渡时，提供持续的液氮供应。我们 将通过实施安装两个Chart MVE Fusion 1500自给自足的具体目标来满足这些需求 并在维斯塔尔研究所创建了一个共享的卫星低温冷凝器农场。新的MVE Fusion 冷冻冷藏室将由Wistar的运营部管理，并由副总裁Luis Montaner博士监督 科学运营总裁兼共享资源副主任代表教职员工在 威斯塔尔学院。维斯塔尔研究所是美国历史最悠久的独立生物医学研究机构，拥有 精力充沛、高度合作的教师，从事癌症生物学、免疫学等前沿研究 和疫苗。该研究所在单抗治疗和癌症方面拥有悠久的研究成功历史 生物学(STELARA®)和疫苗(RotaTeq©)，包括第二种进入临床的新冠肺炎疫苗 在美国进行测试。

Project Summary The goal of this request is to install two innovative Chart MVE Fusion 1500 self-sustaining Cryogenic freezers for -150oC to -190oC temperature long-term storage by creating a shared new cryofreezer farm which will expand the Institute’s cryostorage capabilities. The new cryofeezer farm will support 35 active research labs and core facilities as well as storage of research samples with intellectual property value. As additional freezers cannot be added to the current farm facility due to space constrains and renovations cannot be attempted to expand the footprint of the existing farm, creation of a new cryofreezer farm is critical for Wistar researchers to expand their current usage to support 92 NIH-funded projects and pursue new directions of research and discovery. Two cutting-edge, stand-alone, self-sustaining cryofreezers will enable creation of a new, added cryofreezer farm within the tower basement of the Institute (room B02) and without a need for pipelines providing exogenous replacement of liquid nitrogen. Notably, Chart MVE Fusion self-sustaining freezers utilize innovative Qdrive cryocooling technology to operate on only 50 liters of liquid nitrogen and run solely on electricity after initial liquid nitrogen fill, thus not requiring added replacement of liquid nitrogen. The Chart MVE Fusion 1500 cryogenic Freezer is the world’s first cryofreezer to use patented Qdrive Cryo-cooler technology to remove heat from and reuse existing supplied liquid nitrogen to maintain freezer temperature. By contrast, traditional vapor phase cryofreezers require 80 liters of liquid nitrogen for initial fill and then subsequent daily routine filling to compensate for liquid nitrogen loss due to usage and evaporation. The requested MVE Fusion 1500 cryofreezers will address two scientific needs at the same time: (a) the expansion of cryostorage capabilities with 72 additional storage racks to keep pace with Institute growth, and (b) establish cyofreezer units in a new location without the need to provide constant liquid nitrogen supply as the Institute transitions towards self-sustaining green technology. We will address these needs by implementing a specific aim to install two Chart MVE Fusion 1500 self-sustaining cryofreezers and create a shared satellite cryofreezer farm at The Wistar Institute. The new MVE Fusion cryofreezers will be managed by Wistar’s Department of Operations and overseen by Dr. Luis Montaner, Vice President of Scientific Operations and Associate Director for Shared Resources, on behalf of the faculty at the Wistar Institute. The Wistar Institute is the nation’s oldest independent biomedical research institute, with an energized and highly collaborative faculty who perform cutting-edge research on cancer biology, immunology and vaccines. The Institute boasts a long history of research success in monoclonal antibody therapy and cancer biology (Stelara®) and vaccines (RotaTeq©), including the second COVID-19 vaccine to move into clinical testing in the United States.