Purchase a Beckman Coulter MoFlo Astrios Flow Cytometer

购买 Beckman Coulter MoFlo Astrios 流式细胞仪

基本信息

批准号：
8639790
负责人：
Luis J Montaner
金额：
$ 59.49万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2015-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This is a proposal to acquire a MoFlo Astrios flow cytometer/high-speed cell sorter to replace an old MoFlo Legacy (2001), increasing capacity and adding to capabilities of the current cell sorting facility, a key component for 10 major NIH-funded research programs. The rationale for this request rests on a careful assessment based on the following factors: 1. A need to improve cell sorting service capacity in the Flow Cytometry Facility as crucial for the current NIH-funded investigators and the anticipated growth in Wistar Institute programs. 2. One of 2 sorters, the FACSAria II, is capable of 16 fluorochrome/4 laser detection while the existing MoFlo allows for only five fluorochromes. Upgrading would allow greater flexibility and efficiency for investigators moving from multi-color analysis to sorting, not restricting them to only one sorter. 3. Experiments requiring bio-safety containment at levels greater than BSL-1 are currently restricted to the FACSAria II, resulting in scheduling bottlenecks. The proposed new cell sorter would be appropriately equipped to allow for BSL-2+ sorting. 4. Fluorescent protein sorting is currently limited, given the lack of appropriate laser excitation. The proposed sorter would have a laser in the green/yellow range for optimal excitation of fluorescent proteins such as mCherry, mTomato, etc. 5. The proposed sorter would be capable of six-way sorting, providing the ability to separate more populations than the current four, along with the ability to sort into slides or plates up to 1536 wells. 6. Te existing MoFlo Legacy is still capable of performing well and used on a mostly daily basis, and is preferred by many for its speed and sample recovery, but is suffering from more frequent and increasing amounts of downtime due to age-related repairs. This results in increasing amounts of operator time and places NIH-funded projects at risk due to the potential for longer downtimes due to availability of only one sorter. While major overhaul and enhancement is an option, the expense involved would be better applied into a new instrument. This well-regarded facility, established over 30 years ago, is currently staffed by three experienced technicians and monitored by a faculty user committee. Many investigators at Wistar and neighboring institutions routinely use the cell sorting services, with the existing sorters often heavily booked with occasional backlogs due to capacity limitations as discussed above. Therefore, we propose to upgrade sorting capacity to maintain state- of-the-art support for 10 major and 8 minor users, with 41 R01/P01/U01 NIH-funded research awards, through purchase of a well-equipped MoFlo Astrios high-speed sorter, with appropriate bio-safety level containment, six-way sorting, and the ability to sort single cells directly into tissue culture plates or slides.

描述（由申请人提供）：这是获取Moflo Altrios流式细胞仪/高速细胞分选项以取代旧的Moflo Legacy（2001），增加容量并增加当前单元格分类设施的能力，这是10个由NIH资助的10个主要资金资助的研究计划的关键组成部分。该请求的基本原理取决于基于以下因素的仔细评估：1。需要提高流式细胞仪设施中的细胞分类服务能力，这对于当前资助的NIH资助的研究者以及Wistar Institute计划的预期增长至关重要。 2。两个划分角之一，即Facsaria II，能够16个荧光色素/4激光检测，而现有的MOFLO仅允许使用5个荧光染料。升级将使研究人员从多色分析到分类，而不是仅限于一个分类器，可以提高灵活性和效率。 3。当前需要大于BSL-1的生物安全遏制的实验限制在Facsaria II，从而导致安排瓶颈。提出的新细胞分系程序将适当配备以进行BSL-2+排序。 4。鉴于缺乏适当的激光激发，目前有限荧光蛋白分选。所提出的分类器将在绿色/黄色范围内具有激光器，以最佳激发荧光蛋白，例如MCHERRY，mtomato等。 6。现有的Moflo遗产仍然能够表现良好，并且大部分每天都可以使用，并且由于其速度和样本恢复而受到许多人的优先使用，但由于与年龄相关的维修，其速度和较高的停机量更高。这导致运营商的时间增加，并且由于仅有一个划线器的可用性，可能会导致NIH资助的项目处于危险之中。尽管大修和增强是一种选择，但涉及的费用将更好地应用于新工具中。这个备受赞誉的设施成立于30多年前，目前由三名经验丰富的技术人员组成，并由教师用户委员会监视。 Wistar和邻近机构的许多调查人员通常使用细胞分类服务，由于上述能力限制，现有的分类器通常会大量预订偶尔的积压。因此，我们建议通过购买设备齐全的MOFLO ASTRIOS高速分隔器，并具有适当的生物安全级别容器，将适当的MOFLO ASTRIOS高速分类器购买，并具有适当的生物安全级别的分类，并将单个细胞分类为单个细胞或STLATES CRAPTIES CRAPTIES CRAPTIES CRAPTIES或SLATES CRAPTIES CLELATES CALTISE CRAPETARES或STLATES SLEDATES，我们建议使用41 R01/P01/U01 NIH资助的研究奖，以维持对10名主要和8个未成年人的状态支持，以保持对10个主要和8个未成年人的状态支持。