Molecular Screening Shared Resouce

分子筛选共享资源

• 批准号: 7944609
• 负责人: Kenneth Alan Bradley
• 金额: $ 14.96万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-02 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944609
• 关键词: Animal Model; Area; Biochemical; Biological; Biological Assay; Budgets; California; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cellular biology; Characteristics; Community Clinical Oncology Program; Consultations; Development; Equipment; Funding; Gene Expression Regulation; Genitourinary system; Hematopoietic Neoplasms; Human; Incubators; Individual; Institutes; Instruction; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Knock-out; Leadership; Letters; Libraries; Molecular; Molecular Epidemiology; Mus; Proteins; RNA Sequences; RNA library; Reader; Recording of previous events; Reporting; Resource Sharing; Resources; Robotics; Running; Science; Screening procedure; Signal Transduction; Technology; Therapeutic; Thoracic Oncology; United States National Institutes of Health; Validation; Yeasts; Zebrafish; cancer cell; carcinogenesis; college; follow-up; genome sequencing; genome wide association study; genome-wide; high throughput screening; medical schools; meetings; member; nanosystems; programs; small molecule; small molecule libraries; tumor immunology

The Molecular Screening Shared Resource (MSSR): Completion of the genome sequences of humans, mice, zebrafish and a number of other model organisms has led to the tentative identification of all encoded proteins in these species. The proliferation both of chemical libraries and of libraries of genome wide inhibitory RNA sequences for a number of species has made it possible to conceive of looking for inhibitory small molecules and inhibitory RNAs for all genetically encoded targets. The concomitant development of High Throughput Screening (NTS) technologies has made it possible - with the right resources - to move this from concept to practice. The UCLA JCCC Molecular Screening Shared Resource (MSSR) was established in 2003 to meet this exciting scientific objective. The MSSR now has dedicated space in the new California NanoSystems Institute, small molecule libraries containing over 70,000 compounds, genomewide knockout libraries for yeast, and interfering RNA libraries for both murine and human targets. Excellent academic and technical leadership has established a robust, accessible screening center that assists both in developing reliable and robust enzymatic and cell culture HTS assays and in facilitating genome-wide screens. The David Geffen School of Medicine, the California NanoSystems Institute (CNSI), the UCLA College of Letters and Science, several individual departments and some private donors have joined the JCCC to provide funding for the equipment and libraries (well over $1,000,000) and operating "start-up" required to get the MSSR up and running. The Shared Resource has run (or is currently running) 65 screens, the great majority of which are for JCCC members. Members of the Gene Regulation, Signal Transduction and Therapeutics, Tumor Immunology, Cancer Cell Biology, Hematopoietic Malignancies, Developing Program in Molecular Epidemiology and Carcinogenesis, Thoracic Oncology and Genitourinary Oncology Program Areas have already utilized this Shared Resource. The CCSG budget request for this Shared Resource for the first year CCSG budget is for 13% of the estimated operating budget; support for the shared resource also comes from the CNSI, an NIH Center for Radioprotectors, the UCLA Chancellor's Biosciences Initiative, user recharges and philanthropy. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). Eight Cancer Center members representing four Cancer Center Program Areas completed screens in the Molecular Screening Shared Resource during the reporting period; many screens are ongoing. This is a continuing shared resource.

分子筛选共享资源 (MSSR)：完成人类基因组序列， 小鼠、斑马鱼和许多其他模式生物已导致所有编码的初步鉴定 这些物种中的蛋白质。化学文库和全基因组文库的增殖 许多物种的抑制性RNA序列使得寻找抑制性RNA成为可能 针对所有基因编码靶标的小分子和抑制性 RNA。随之而来的发展 高通量筛选 (NTS) 技术使得在适当的资源下移动成为可能 这是从概念到实践。加州大学洛杉矶分校 JCCC 分子筛选共享资源 (MSSR) 成立于 2003 年，旨在实现这一令人兴奋的科学目标。 MSSR 现在在 新的加州纳米系统研究所，包含超过 70,000 种全基因组化合物的小分子库 酵母的敲除文库，以及鼠类和人类靶标的干扰 RNA 文库。出色的 学术和技术领导层建立了一个强大的、易于使用的筛选中心，协助 开发可靠且强大的酶和细胞培养 HTS 测定并促进全基因组 屏幕。大卫格芬医学院、加州纳米系统研究所 (CNSI)、加州大学洛杉矶分校 文学与科学学院、几个单独的院系和一些私人捐助者已加入 JCCC 将为设备和图书馆提供资金（远超过 1,000,000 美元）和运营“启动” 启动并运行 MSSR 所需的。共享资源已运行（或当前正在运行） 65 屏幕，其中绝大多数是为 JCCC 会员提供的。基因调控、信号成员 转导和治疗、肿瘤免疫学、癌细胞生物学、造血系统恶性肿瘤、 分子流行病学和癌变、胸部肿瘤学和泌尿生殖学发展项目 肿瘤学计划领域已经利用了该共享资源。 CCSG 对此的预算请求 CCSG 第一年的共享资源预算占预计运营预算的 13%；支持 共享资源还来自 CNSI、NIH 辐射防护中心、加州大学洛杉矶分校校长的 生物科学倡议、用户充值和慈善事业。 （另请参阅第 6.2.3 节“共享” 历史、描述、基本特征中的资源）。 代表四个癌症中心项目领域的八名癌症中心成员完成了筛选 报告期内的分子筛选共享资源；许多屏幕正在进行中。 这是一个持续的共享资源。