Vector Shared Resource

矢量共享资源

• 批准号: 7944613
• 负责人: NORIYUKI KASAHARA
• 金额: $ 11.59万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-02 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944613
• 关键词: Adenovirus Vector; Animal Experiments; Applications Grants; Area; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cellular biology; Characteristics; Consultations; Custom; Development; Disease; Dominant-Negative Mutation; Epitopes; Gene Delivery; Gene Expression Regulation; Gene Transfer; Genes; Genetic; Genitourinary system; Hematopoietic Neoplasms; In Vitro; Individual; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Laboratories; Libraries; Malignant - descriptor; Malignant Neoplasms; Mammalian Cell; Methodology; Molecular Epidemiology of Cancer; Organogenesis; Pathogenesis; Preparation; Production; Recording of previous events; Reporting; Research; Research Activity; Research Personnel; Resource Sharing; Resources; Services; Signal Transduction; Source; Techniques; Technology; Therapeutic; Thoracic Oncology; Transgenes; Translational Research; Viral Vector; Woman; Work; anticancer research; cancer cell; cellular engineering; cost; design and construction; experience; gene therapy; in vivo; interest; meetings; member; molecular imaging; mutant; new therapeutic target; oncology; programs; research study; tumor immunology; vector

The Vector Shared Resource (VSR). Advances in techniques for efficient cellular engineering using virusbased gene delivery vehicles ("vectors") have made it feasible to utilize gene transfer as a methodology for elucidating functions of specific genes, by examining consequences of their over-expression or inhibition. The Vector Shared Resource is a new Shared Resource, established to meet the increasing demand both for routine construction and production of viral vectors and for assistance in developing new vectors, both for cell culture and in vivo animal experiments. The objective of the Vector Shared Resource is to promote and Facilitate basic and translational research by providing JCCC investigators with access to vector technologies that enable efficient gene transfer to mammalian cells, both in culture and in vivo. Our services include: (1) provision, at minimal cost, of various pre-made retroviral, lentiviral and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing various mammalian genes and corresponding inhibitory sequences; (2) construction and production of custom viral vectors that contain a specific sequence(s) of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers; and (3) provision of educational and advisory resources for researchers with limited experience in viral vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in vitro and/or in vivo. As the use of viral vector technology requires specialized expertise and resources often not found in an individual investigator's laboratory, offering easy access to these technologies, consolidated in the form of a Shared Resource, can significantly facilitate and expand the scope of JCCC research activities. Furthermore, our services are more cost-effective than utilizing the limited commercial sources offering such technologies. Further value is added by customized technical support available from accessible and knowledgeable Shared Resource staff who can work closely with investigators to troubleshoot and optimize gene transfer experiments, assist with institutional regulatory compliance documents and grant proposal submissions and who are actively engaged in development and application of new vector technologies. Initiated in 2003, the Vector Shared Resource has been used by members of the Signal Transduction and Therapeutics, Women's Cancer, Hematopoietic Malignancies, Gene Regulation, Cancer Cell Biology, Tumor Immunology, Thoracic Oncology, Genitourinary Oncology, Molecular Epidemiology and Cancer Molecular Imaging Program Areas for consultation, vector construction, small and large scale preparation of vector stocks and additional services. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). 25 Cancer Center members representing 10 Cancer Center Program Areas utilized the services of the Vector Shared Resource during the reporting period. This is a new shared resource.

矢量共享资源（VSR）。基于病毒的高效细胞工程技术的进展 基因递送载体（"载体"）使得利用基因转移作为一种方法学 通过检查其过度表达或抑制的后果来阐明特定基因的功能。 矢量共享资源是一种新的共享资源，建立以满足日益增长的需求， 用于病毒载体的常规构建和生产以及用于开发新载体的辅助， 细胞培养和体内动物实验。矢量共享资源的目标是促进和 通过向JCCC研究人员提供载体技术，促进基础和转化研究 其能够在培养物中和体内将基因有效地转移到哺乳动物细胞中。我们的服务包括：（1） 以最低成本提供各种预制的逆转录病毒、慢病毒和腺病毒载体储备， 用于初步实验的标准标记基因，以及可用载体库 表达各种哺乳动物基因和相应的抑制序列;（2）构建和 生产含有感兴趣的特定序列（包括野生型和 具有或不具有表位标签的突变cDNA，显性负表达构建体，反义mRNA， siRNA等）（3）为个人研究人员提供教育和咨询资源， 在病毒载体技术方面经验有限，但希望利用这些技术 目的基因序列在体外和/或体内的有效功能性表达。由于病毒的使用 病媒技术需要专门的专门知识和资源， 实验室，提供方便地访问这些技术，以共享资源的形式整合，可以 大大促进和扩大JCCC研究活动的范围。此外，我们的服务是 这比利用提供这种技术的有限商业来源更具成本效益。进一步的价值是 通过可访问和知识渊博的共享资源工作人员提供的定制技术支持进行添加 谁可以与研究人员密切合作，以解决和优化基因转移实验，协助 机构监管合规文件和赠款提案提交，并积极 从事新型病媒技术的开发和应用。2003年，Vector Shared 资源已被信号转导和治疗学，妇女癌症， 造血系统恶性肿瘤，基因调控，癌细胞生物学，肿瘤免疫学，胸 肿瘤学、泌尿生殖系统肿瘤学、分子流行病学和癌症分子成像计划领域 用于咨询、病媒构建、小规模和大规模病媒储备的制备以及 服务（另请参见第6.2.3节关于共享资源的历史，描述，基本 特征）。 代表10个癌症中心项目区的25名癌症中心成员利用了 报告所述期间的向量共享资源。这是一个新的共享资源。