Vector Shared Resource

向量共享资源(VSR)。基于病毒的高效细胞工程技术研究进展 基因传递载体(“载体”)已经使利用基因转移作为一种方法学成为可能。 通过检查特定基因过度表达或抑制的后果来阐明特定基因的功能。 矢量共享资源是一种新的共享资源，是为满足日益增长的需求而建立的 用于常规构建和生产病毒载体，并协助开发新的载体，这两种方法都适用于 细胞培养和体内动物实验。矢量共享资源的目标是促进和 通过向JCCC调查人员提供对载体技术的访问，促进基础研究和翻译研究 这使得高效的基因转移到哺乳动物细胞，无论是在培养中还是在体内。我们的服务包括：(1) 以最低成本提供各种预先制成的逆转录病毒、慢病毒和腺病毒载体库存，表达 在初步实验中使用的标准标记基因，以及可用的载体文库 表达多种哺乳动物基因和相应的抑制序列；(2)构建和 生产包含特定序列(S)的定制病毒载体(包括野生型和 具有或不具有表位标签、显性负表达构建体、反义RNA、 SiRNA等)为个别研究人员提供教育和咨询资源；及 在病毒载体技术方面经验有限的研究人员，但希望利用此类技术 在体外和/或体内高效地表达感兴趣的基因序列。因为病毒的使用 媒介技术需要专门的专业知识和资源，而这些专业知识和资源往往是个别研究人员所不具备的 实验室以共享资源的形式提供对这些技术的轻松访问，可以 极大地促进和扩大JCCC研究活动的范围。此外，我们的服务是 比利用提供此类技术的有限商业来源更具成本效益。进一步的价值是 由可访问且知识渊博的共享资源员工提供的定制技术支持添加 世卫组织可以与调查人员密切合作，排查和优化基因转移实验，协助 机构法规遵从性文件和拨款提案提交，并积极 从事新载体技术的开发和应用。发起于2003年，向量共享 资源已经被信号转导和治疗，妇女癌症， 恶性血液病，基因调控，癌细胞生物学，肿瘤免疫学，胸科 肿瘤学、泌尿生殖肿瘤学、分子流行病学和癌症分子成像计划领域 用于咨询、病媒建设、小规模和大规模的病媒股票准备以及额外的 服务。(另请参阅历史、描述、基本中关于共享资源的第6.2.3节 特征)。 代表10个癌症中心计划地区的25名癌症中心成员利用 在本报告所述期间，向量共享资源。这是一个新的共享资源。

The Vector Shared Resource (VSR). Advances in techniques for efficient cellular engineering using virusbased gene delivery vehicles ("vectors") have made it feasible to utilize gene transfer as a methodology for elucidating functions of specific genes, by examining consequences of their over-expression or inhibition. The Vector Shared Resource is a new Shared Resource, established to meet the increasing demand both for routine construction and production of viral vectors and for assistance in developing new vectors, both for cell culture and in vivo animal experiments. The objective of the Vector Shared Resource is to promote and Facilitate basic and translational research by providing JCCC investigators with access to vector technologies that enable efficient gene transfer to mammalian cells, both in culture and in vivo. Our services include: (1) provision, at minimal cost, of various pre-made retroviral, lentiviral and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing various mammalian genes and corresponding inhibitory sequences; (2) construction and production of custom viral vectors that contain a specific sequence(s) of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers; and (3) provision of educational and advisory resources for researchers with limited experience in viral vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in vitro and/or in vivo. As the use of viral vector technology requires specialized expertise and resources often not found in an individual investigator's laboratory, offering easy access to these technologies, consolidated in the form of a Shared Resource, can significantly facilitate and expand the scope of JCCC research activities. Furthermore, our services are more cost-effective than utilizing the limited commercial sources offering such technologies. Further value is added by customized technical support available from accessible and knowledgeable Shared Resource staff who can work closely with investigators to troubleshoot and optimize gene transfer experiments, assist with institutional regulatory compliance documents and grant proposal submissions and who are actively engaged in development and application of new vector technologies. Initiated in 2003, the Vector Shared Resource has been used by members of the Signal Transduction and Therapeutics, Women's Cancer, Hematopoietic Malignancies, Gene Regulation, Cancer Cell Biology, Tumor Immunology, Thoracic Oncology, Genitourinary Oncology, Molecular Epidemiology and Cancer Molecular Imaging Program Areas for consultation, vector construction, small and large scale preparation of vector stocks and additional services. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). 25 Cancer Center members representing 10 Cancer Center Program Areas utilized the services of the Vector Shared Resource during the reporting period. This is a new shared resource.