Laser microdissection for molecular analysis of oral, dental and craniofacial dis

激光显微切割用于口腔、牙齿和颅面疾病的分子分析

• 批准号: 7792854
• 负责人: XIAOFENG ZHOU
• 金额: $ 23.75万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-20 至 2011-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792854
• 关键词: Aging; Arts; Biological Markers; Cells; Chicago; Cicatrix; Connective Tissue; DNA damage checkpoint; Dental; Dental Enamel; Dentistry; Development; Enamel Formation; Epithelial; Evolution; Funding; Housing; Illinois; Lasers; Malignant neoplasm of liver; Medicine; Microdissection; Minor; Molecular; Molecular Analysis; Neoplasm Metastasis; Oral; Pathology; Pathway interactions; Periodontal Ligament; Research Personnel; Research Project Grants; Rhabdomyosarcoma; Sampling; Serpins; Stifle joint; Stress; System; Tissues; Universities; college; craniofacial; experience; interest; malignant mouth neoplasm; microbial; tumor progression

DESCRIPTION (provided by applicant): Laser microdissection (LMD) makes it possible to distinguish between relevant and non-relevant cells or tissues and enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material. Researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are relevant, reproducible, and specific. The existing multi-user LMD system at University of Illinois Chicago (UIC) is housed and operated by Transdisciplinary Pathology Facility at Department of Pathology, College of Medicine, but a new system is needed, because: 1) the existing Leica AS LMD Laser Microdissection System is aging, and 2) is heavily used - serving the entire UIC campus. A dedicated LMD system is needed for 8 major NIH-funded projects as well as minor projects, some also NIH-funded, at UIC College of Dentistry. These investigators have much experience in LMD, but are stifled by the limitations of our existing system. A state-of-the-art LMD system will greatly facilitate the scientific progress of these research projects. Major user projects include: 1) biomarker identification for oral cancer progression and metastasis, 2) DNA damage checkpoint pathways in oral cancer and liver cancer, 3) dental enamel formation and evolution, 4) molecular analysis of stress impaired microbial clearance, 5) ameloblastin function in periodontal development and the periodontal ligament, 6) epithelial-connective tissue interactions and scar formation, 7) molecular mechanisms of serpins in oral cancer tumorgenesis, 8) molecular analysis of rhabdomyosarcoma.

描述（由申请人提供）：激光显微切割（LMD）可以区分相关和非相关细胞或组织，并使研究人员能够从异质起始材料中获得均匀的超纯样品。研究人员可以选择性地和常规地分析感兴趣的区域，直到单个细胞，以获得相关，可重复和特异性的结果。伊利诺伊大学芝加哥（UIC）现有的多用户LMD系统由医学院病理学系的跨学科病理学机构安装和操作，但需要一个新系统，因为：1）现有的Leica AS LMD激光显微切割系统正在老化，2）被大量使用-服务于整个UIC校园。UIC牙科学院的8个主要NIH资助项目以及一些次要项目（也是NIH资助的）需要专用LMD系统。这些调查人员在LMD方面有很多经验，但受到我们现有系统的限制。先进的LMD系统将极大地促进这些研究项目的科学进展。主要用户项目包括：1）口腔癌进展和转移的生物标志物鉴定，2）口腔癌和肝癌中的DNA损伤检查点途径，3）牙釉质形成和进化，4）应力受损的微生物清除的分子分析，5）成釉细胞蛋白在牙周发育和牙周韧带中的功能，6）上皮-结缔组织相互作用和瘢痕形成，7）丝氨酸蛋白酶抑制剂在口腔癌发生中的分子机制; 8）横纹肌肉瘤的分子分析。