High-resolution ex vivo micro-computerized tomography system

描述（由申请人提供）：本申请的主要目的是购买一台离体标本微计算机断层扫描（ex vivo microCT）仪器。我们建议购买SkyScan 1172微型ct系统，该系统将为天普大学16个联邦资助研究项目（14个NIH， 1个NIOSH， 1个DOE）中的11个联邦资助研究人员（10个NIH， 1个DOE）提供服务。我们的大学缺乏任何类型的微ct系统，尽管我们的需求很大，而且还在不断增长。在拟议的应用程序中的仪器将允许优良的精细结构的分辨率，即使在低对比度的样品，因此微型ct的新应用可以适应。SkyScan 1172可以对标本进行高分辨率成像——小于15m体素大小，低对比度分辨率为55m。一次典型的扫描需要30分钟到1小时，该系统配备了一个集群pc的重建工作站和特殊的软件，可以快速计算每张图像幻灯片的信息，这样用户就可以在扫描下一个样本时重建扫描。这将提供所需的高通量，以满足我们的研究界对microCT的需求。数字数据允许对骨、软骨、肌肉、脂肪、脉管系统和肿瘤的各种结构参数进行定量测定。这台高分辨率的微型ct仪器将被安置在我们的健康科学校园成像中心。microCT的加入将大大增强现有的成像服务，并将补充该中心已经存在的基于光的荧光技术。该仪器将由从医学院、牙科学院、工程学院、卫生专业学院和科学技术学院的用户中选出的教员委员会管理。提出的高分辨率微ct SkyScan 1172仪器的应用包括：1)由重复性和强强度工作任务引起的骨退行性变化的成像和量化，以及干预的有效性。2)基因工程小鼠（转基因和敲除模型）、病理性骨重塑（如卵巢切除和低钙饮食）和头部创伤骨折后骨结构的成像和量化研究。3)早期发现龋齿，制定预防和治疗措施。4)牙科合成复合材料力学性能的研究，旨在改进牙科修复材料。5)研究脑卒中和心血管疾病的血管形成、动脉粥样硬化及其影响因素。6)评估肝、肺疾病和癌症治疗方法的有效性。收购SkyScan 1172对于这些和未来需要这种最先进技术的项目的持续进展至关重要。

DESCRIPTION (provided by applicant): The primary objective of this proposal is to purchase an ex vivo specimen microcomputed tomography (ex vivo microCT) instrument. We are proposing the purchase of a SkyScan 1172 microCT system, which will serve 11 federally funded investigators (10 NIH, 1 Department of Energy (DOE)) in 16 federally funded research projects (14 NIH, 1 NIOSH, 1 DOE) at Temple University. Our university lacks any type of microCT system, although our need is large and continues to grow. The instrument in the proposed application will allow excellent resolution of fine structure even in low contrast samples, so that new applications for microCT can be accommodated. The SkyScan 1172 can image specimens with high-resolution - less than 1 5m voxel size and low-contrast resolution of 5 5m. A typical scan takes 30 min to 1 hr, and the system comes with a reconstruction workstation of clustered PCs and special software to rapidly calculate information from each image slide, so that a user can reconstruct a scan while the next specimen is scanning. This will provide the high throughput required to service the needs of our research community for microCT. The digital data then permits quantitative determination of a variety of structural parameters in bone, cartilage, muscle, fat, vasculature and tumors. This high-resolution microCT instrument will be housed at our Health Science campus in the Imaging Center. The addition of microCT will significantly enhance the available imaging services and will complement the light-based, fluorescence technologies that already exist in this center. The instrument will be administered by a committee of faculty members drawn from the users from the Schools/Colleges of Medicine, Dentistry, Engineering, Health Professions, and Science and Technology. Applications for the proposed high-resolution microCT SkyScan 1172 instrument include: 1) Imaging and quantification of bone degenerative changes induced by performing repetitive and forceful work tasks, and the effectiveness of interventions. 2) Imaging and quantification of bone architecture in studies of genetically engineered mice (transgenic and knockout models), pathological bone remodeling (such as after ovariectomy and low calcium diet), and after head trauma fracture. 3) Detection of the earliest stages of dental caries and the development of preventative and treatment interventions. 4) Examination of mechanical properties of synthetic dental composites with the goal of improving dental restorative materials. 5) To study vascular formation and atherosclerosis in, and contributing factors of, stroke and cardiovascular disease. 6) To assess effectiveness of therapeutic approaches for liver and lung diseases and cancer. The acquisition of the SkyScan 1172 is essential for continued progress of these and future projects requiring this state-of-the-art technology.