High-resolution ex vivo micro-computerized tomography system

描述（由申请人提供）：本提案的主要目的是购买离体标本显微计算机断层扫描（ex vivo microCT）仪器。我们建议购买SkyScan 1172 microCT系统，该系统将在坦普尔大学的16个联邦资助的研究项目（14个NIH，1个NIOSH，1个DOE）中为11个联邦资助的研究人员（10个NIH，1个能源部（DOE））提供服务。我们的大学缺乏任何类型的microCT系统，虽然我们的需求很大，并继续增长。在拟议的应用中的仪器将允许优良的分辨率的精细结构，即使在低对比度的样品，使新的应用microCT可以容纳。SkyScan 1172可以对标本进行高分辨率成像-体素尺寸小于1.5 m，低对比度分辨率为5.5 m。一次典型的扫描需要30分钟到1小时，该系统配备了一个由集群PC和特殊软件组成的重建工作站，可以快速计算每个图像载玻片的信息，因此用户可以在扫描下一个标本时重建扫描。这将提供高吞吐量，以满足我们研究界对microCT的需求。然后，数字数据允许定量测定骨、软骨、肌肉、脂肪、脉管系统和肿瘤中的各种结构参数。这种高分辨率的microCT仪器将被安置在我们的健康科学园区的成像中心。microCT的加入将显著增强现有的成像服务，并将补充该中心现有的基于光的荧光技术。该仪器将由来自医学、牙科、工程、卫生保健和科学技术学校/学院的教师组成的委员会管理。 所提出的高分辨率microCT SkyScan 1172仪器的应用包括：1）通过执行重复性和强有力的工作任务以及干预的有效性引起的骨退行性变化的成像和量化。2)在基因工程小鼠（转基因和敲除模型）、病理性骨重建（如卵巢切除术和低钙饮食后）和头部创伤骨折后的研究中，骨结构的成像和定量。3)检测龋齿的早期阶段，并制定预防和治疗干预措施。4)以改善牙科修复材料为目标的合成牙科复合材料的机械性能检查。5)研究中风和心血管疾病中的血管形成和动脉粥样硬化及其影响因素。6)评估肝、肺疾病和癌症治疗方法的有效性。 收购SkyScan 1172对于这些项目以及未来需要这种最先进技术的项目的持续进展至关重要。

DESCRIPTION (provided by applicant): The primary objective of this proposal is to purchase an ex vivo specimen microcomputed tomography (ex vivo microCT) instrument. We are proposing the purchase of a SkyScan 1172 microCT system, which will serve 11 federally funded investigators (10 NIH, 1 Department of Energy (DOE)) in 16 federally funded research projects (14 NIH, 1 NIOSH, 1 DOE) at Temple University. Our university lacks any type of microCT system, although our need is large and continues to grow. The instrument in the proposed application will allow excellent resolution of fine structure even in low contrast samples, so that new applications for microCT can be accommodated. The SkyScan 1172 can image specimens with high-resolution - less than 1 5m voxel size and low-contrast resolution of 5 5m. A typical scan takes 30 min to 1 hr, and the system comes with a reconstruction workstation of clustered PCs and special software to rapidly calculate information from each image slide, so that a user can reconstruct a scan while the next specimen is scanning. This will provide the high throughput required to service the needs of our research community for microCT. The digital data then permits quantitative determination of a variety of structural parameters in bone, cartilage, muscle, fat, vasculature and tumors. This high-resolution microCT instrument will be housed at our Health Science campus in the Imaging Center. The addition of microCT will significantly enhance the available imaging services and will complement the light-based, fluorescence technologies that already exist in this center. The instrument will be administered by a committee of faculty members drawn from the users from the Schools/Colleges of Medicine, Dentistry, Engineering, Health Professions, and Science and Technology. Applications for the proposed high-resolution microCT SkyScan 1172 instrument include: 1) Imaging and quantification of bone degenerative changes induced by performing repetitive and forceful work tasks, and the effectiveness of interventions. 2) Imaging and quantification of bone architecture in studies of genetically engineered mice (transgenic and knockout models), pathological bone remodeling (such as after ovariectomy and low calcium diet), and after head trauma fracture. 3) Detection of the earliest stages of dental caries and the development of preventative and treatment interventions. 4) Examination of mechanical properties of synthetic dental composites with the goal of improving dental restorative materials. 5) To study vascular formation and atherosclerosis in, and contributing factors of, stroke and cardiovascular disease. 6) To assess effectiveness of therapeutic approaches for liver and lung diseases and cancer. The acquisition of the SkyScan 1172 is essential for continued progress of these and future projects requiring this state-of-the-art technology.