Becton Dickinson FACSAria IIu Cell Sorter Flow Cytometer
Becton Dickinson FACSAria IIu 细胞分选仪 流式细胞仪
基本信息
- 批准号:7794687
- 负责人:
- 金额:$ 49.48万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-07-22 至 2012-07-21
- 项目状态:已结题
- 来源:
- 关键词:ArtsCell SeparationCell physiologyCellsCollaborationsCommunicable DiseasesContainmentCore FacilityDevelopmentFlow CytometryFosteringFundingGenetic RecombinationGoalsGrantGrowthHumanImmuneImmune systemInflammatoryMalignant NeoplasmsMedicalMethodsMonitorMusNeoplasm MetastasisNeurodegenerative DisordersOrganPhenotypePopulationProceduresReporterResearchResearch PersonnelResearch Project GrantsSorting - Cell MovementSpeedStem Cell ResearchStem cellsSystemTechnologyTranslational ResearchUnited States National Institutes of HealthVirusVirus DiseasesVirus ReplicationWorkanimal tissuebasecancer stem celldesigninnovationinstrumentinstrumentationnovel strategiespathogenprecursor celltumortumor initiationvirology
项目摘要
DESCRIPTION (provided by applicant): The major goal of the current proposal is to obtain funds for the purchase of a Becton Dickinson FACSAria II Cell Sorter Flow Cytometer (BD-FACS) system, including a Becker BioPROtect III Bio-containment enclosure that is specifically designed for analyses on human and infectious materials. Flow cytometry is a unique and powerful technology for analyzing the phenotype and function of cells, as well as for isolating defined cell populations by sorting. In recent years, the use of flow cytometric analyses in virology research has become ever more prominent as newer approaches for dissecting the interactions between viruses and cells of the immune system have been developed. Fluorescent-based reporter systems have been developed to monitor virus replication, reactivation, and recombination, and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. In cancer and stem cell research, cell sorting by flow cytometry is the state-of-the-art method to determine and characterize the precursor cells for organ development as well as for tumor initiation, growth, and metastasis. We propose to purchase this instrument in conjunction with the Medical of Georgia (MCG) Flow Cytometry Core Facility (FACS Core). The instrument will initially support several projects of a central group of at least 18 established investigators funded by NIH grants. The needs of these projects are varied and include FACS sorting of immune cell populations from virally infected mice, and the purification of human or mouse stem cells (organ or tumor-type-specific) as well as inflammatory cell populations associated with viral infections, cancer development, and neurodegenerative diseases. The instrument will be also available to support the research projects of many other investigators at MCG, especially in procedures that require sorting of biohazardous materials. Thus, the acquisition of this cell sorter system will significantly enhance and accelerate progress in NIH-funded projects, especially those in which separation and functional analysis of cell populations from human and animal tissues exposed to pathogens (viruses) need to be analyzed. It will significantly elevate the limiting capacity of the MCG FACS facility in cell sorting of non-infectious materials due to the Core's heavy use by many investigators. We also aim to promote innovation by working with users and fostering collaborations and cross-core partnerships that encourage cutting-edge basic and translational research in infectious diseases, cancer, and neurodegenerative diseases as well as stem-cell research.
描述(由申请人提供):当前提案的主要目标是获得资金,用于购买Becton Dickinson FACSAria II细胞分选流式细胞仪(BD-FACS)系统,包括专门设计用于分析人体和感染性材料的Becker BioPROtect III生物防护外壳。流式细胞术是一种独特而强大的技术,用于分析细胞的表型和功能,以及通过分选分离确定的细胞群。近年来,随着用于解剖病毒与免疫系统细胞之间相互作用的新方法的开发,流式细胞术分析在病毒学研究中的应用变得越来越突出。基于荧光素的报告系统已被开发用于监测病毒的复制、再活化和重组,并且仪器的复杂技术进步为高速多色分析和细胞分选提供了新的平台。在癌症和干细胞研究中,通过流式细胞术进行细胞分选是确定和表征用于器官发育以及用于肿瘤起始、生长和转移的前体细胞的最先进方法。我们建议与格鲁吉亚医学中心(MCG)流式细胞仪核心设施(FACS Core)一起购买该仪器。该工具最初将支持由NIH赠款资助的至少18名既定研究人员组成的中心小组的几个项目。这些项目的需求各不相同,包括对病毒感染小鼠的免疫细胞群进行FACS分选,以及对人或小鼠干细胞(器官或肿瘤类型特异性)以及与病毒感染、癌症发展和神经退行性疾病相关的炎性细胞群进行纯化。该仪器还将用于支持MCG许多其他研究人员的研究项目,特别是在需要对生物危害材料进行分类的程序中。因此,该细胞分选系统的收购将显著提高和加速NIH资助项目的进展,特别是那些需要分析暴露于病原体(病毒)的人类和动物组织细胞群的分离和功能分析的项目。由于许多研究者大量使用Core,它将显著提高MCG FACS设施在非感染性材料细胞分选方面的有限能力。我们还旨在通过与用户合作,促进合作和跨核心伙伴关系,鼓励传染病,癌症和神经退行性疾病以及干细胞研究的前沿基础和转化研究,以促进创新。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Dimitrios Moskofidis其他文献
Dimitrios Moskofidis的其他文献
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Function of inducible HSP70 genes in mouse model
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6780900 - 财政年份:2001
- 资助金额:
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Function of HSPs in mouse models for human diseases
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- 资助金额:
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7268225 - 财政年份:2001
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