HTC Imaging Plate Detector and Crystal Cryo-Cooling System

HTC 成像板探测器和晶体冷冻系统

• 批准号: 7793259
• 负责人: Todd O Yeates
• 金额: $ 45.53万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2011-07-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793259
• 关键词: 14 year old; Arts; Biochemical; Biological; Blood Clot; Blood coagulation; Complex; Crystallography; Data; Data Collection; Development; Disease; Educational process of instructing; Equipment; Health; Heart Diseases; Image; Infection; Longevity; Maintenance; Malignant Neoplasms; Methods; Minor; Mission; Molecular; Molecular Structure; N.I.H. Research Support; Neurodegenerative Disorders; Play; Research; Research Project Grants; Research Proposals; Robotics; Roentgen Rays; Role; Scientist; Signal Transduction; Speed; Stream; Structural Biologist; Structure; System; Training; Ursidae Family; Work; X ray diffraction analysis; X-Ray Diffraction; detector; frontier; insight; instrument; instrumentation; microbial; prevent; repaired

DESCRIPTION (provided by applicant): This proposal seeks to upgrade an existing 14 year old X-ray detector with a state-of-the-art HTC imaging plate detector and crystal cryo-cooling system. The instrument will be part of the macromolecular diffraction instrumentation facility at UCLA and will be dedicated to biochemical research supported by the NIH as well as other agencies. The instrumentation will also provide critical training for young scientists. The equipment requested is a state-of-the-art Rigaku HTC Image Plate detector and an X-STREAM 2000 crystal cryocooling system. The need for new instrumentation is driven by new developments in molecular crystallography, combined with the necessity of replacing outdated equipment in order to advance research on frontier problems. The new detector will make it possible to solve challenging molecular structures, particularly in cases where only small crystals can be obtained; this scenario is more and more common as scientists attack increasingly complex molecular structures, and as robotic methods produce crystals at an increasing speed, but often of decreasing size. The new equipment will support six major users and eight minor users working on NIH-supported research projects related to biomedical problems including but not limited to: neurodegenerative diseases, blood clotting, cellular signaling and cancer, heart disease, and microbial infection. New equipment is required to replace an old and outdated RAXIS4 detector which has served well for 14 years of nearly continuous use, but is nearing the end of its lifespan. Maintenance and replacement of worn parts on that detector is becoming problematic. Besides being new and requiring less frequent repair, the new equipment will be more sensitive and will have a faster readout, which will make it possible to obtain high quality diffraction data faster on a larger range of macromolecular crystals. With this important upgrade we will be able to prevent data collection from limiting progress on our scientific research problems. The macromolecular X-ray facility at UCLA is remarkable for the breadth and number of separate research groups it supports, and for the in-depth teaching it provides to large numbers of structural biologists-in- training. The new instrument will make it possible to continue and advance these important missions. HEALTH RELEVANCE: The proposed research advances our understanding of the structures of biological molecules and the roles they play in health and diseases. The fundamental biological insights to be gained will bear on biomedical issues including: neurodegenerative diseases, blood clotting, cellular signaling and cancer, heart disease, microbial infection, and others. To enable that research, this proposal requests X-ray diffraction equipment that will be utilized by at least 14 different research groups.

描述(由申请人提供)：这项建议旨在升级现有的已有14年历史的X射线探测器，配备最先进的HTC成像板探测器和晶体冷冻系统。该仪器将是加州大学洛杉矶分校大分子衍射仪设施的一部分，并将致力于由NIH和其他机构支持的生化研究。该仪器还将为年轻科学家提供关键培训。所要求的设备是一台最先进的Rigaku HTC图像板探测器和一台X-Stream 2000晶体冷冻系统。分子结晶学的新发展推动了对新仪器的需求，同时为了推进对前沿问题的研究，有必要更换过时的设备。新的探测器将使解决具有挑战性的分子结构成为可能，特别是在只能获得小晶体的情况下；随着科学家攻击日益复杂的分子结构，以及机器人方法以越来越快的速度产生晶体，但通常是尺寸减小，这种情况越来越常见。新设备将支持NIH支持的与生物医学问题相关的研究项目的6个主要用户和8个次要用户，这些问题包括但不限于：神经退行性疾病、血液凝固、细胞信号与癌症、心脏病和微生物感染。需要新的设备来取代旧的和过时的RAXIS4探测器，该探测器已经良好地使用了14年，几乎连续使用，但即将结束其寿命。该探测器上磨损部件的维护和更换正变得有问题。除了是新的，需要较少的频繁维修，新的设备将更敏感，将有更快的读数，这将使在更大范围的大分子晶体上更快地获得高质量的衍射数据成为可能。有了这一重要的升级，我们将能够防止数据收集限制我们的科学研究问题的进展。加州大学洛杉矶分校的大分子X射线设备因其支持的独立研究小组的广度和数量以及它为大量正在接受培训的结构生物学家提供的深入教学而引人注目。新的文书将使继续和推进这些重要任务成为可能。 与健康相关：拟议中的研究促进了我们对生物分子结构及其在健康和疾病中所起作用的理解。将获得的基本生物学见解将涉及生物医学问题，包括：神经退行性疾病、血液凝固、细胞信号与癌症、心脏病、微生物感染等。为了实现这项研究，这项提案要求至少14个不同的研究小组使用X射线衍射设备。