Confocal imaging resources for the JHU Zebrafish Center

JHU 斑马鱼中心的共焦成像资源

• 批准号: 7794133
• 负责人: Steven D Leach
• 金额: $ 47万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2011-07-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794133
• 关键词: American; Applications Grants; Biology; Cells; Development; Developmental Biology; Elements; Embryo; Faculty; Funding; Genetic; Genetic Medicine; Goals; Grant; Head; Health; Hour; Human; Image; Institutes; Investigation; Joints; Lead; Life; Maintenance; Malignant Neoplasms; Malignant neoplasm of pancreas; MicroRNAs; Microscope; Microscopy; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; Natural regeneration; Neural Crest; Neuronal Differentiation; Noise; Pancreas; Protocols documentation; Recovery; Regulator Genes; Research Personnel; Resolution; Resources; Role; Scanning; Shipping; Ships; Signal Transduction; System; Time; Tissues; United States National Institutes of Health; Universities; Work; Zebrafish; base; cellular engineering; design; embryo tissue; instrumentation; medical schools; member

DESCRIPTION (provided by applicant): This S10 Shared Instrumentation Grant application seeks funding in support of confocal live imaging resources for the Johns Hopkin University's Center for Functional Investigation in Zebrafish, also known as FinZ Center. FinZ Center was established in 2008 under joint funding from the School of Medicine, the Institute for Cellular Engineering (ICE), and the Johns Hopkins McKusick-Nathans Institute of Genetic Medicine (IGM). The Center is currently led by three scientific co-directors, Steven Leach, Andrew McCallion and Michael Parsons, each of whom is a noted investigator in zebrafish genetics and zebrafish developmental biology. Together with Dr. Joshua Mendell, another IGM faculty member pursuing work in zebrafish, this group will comprise the Major User Group for the proposed Zeiss LSM 710 confocal microscope. These four Major Users are all highly regarded investigators in a variety of scientific fields with direct impact on human health, including pancreatic development, pancreatic cancer, neuronal differentiation, b-cell regeneration, computational prediction of gene regulatory sequences, neural crest biology and the role of microRNAs in human malignancy. Together, they serve as PI's or co-Pis on seven NIH R01 or P01 grants, funded by four different NIH Institutes (NIGM, NICHD, NIDDK and NCI). Based on the Major User Group's common need for live imaging of zebrafish embryos and other tissues in support of their NIH-funded work, this application seeks funding for a Zeiss LSM 710 3-channel spectral confocal system, with an environmental control system to support long-term imaging of zebrafish embryos, tissues and cells. Justification for this request is based on the following documented elements: 1) the Zeiss 710 system offers an unprecedented signal-to-noise ratio associated with a unique scan head design, providing the opportunity to apply high resolution spectral imaging to deep-seated zebrafish tissues; 2) the fact that Johns Hopkins Microscopy Core resources here at Johns Hopkins are already fully subscribed and therefore cannot accommodate the 10-36 hour time lapse imaging protocols required by the Major User Group; and 3) consistent with the broader goals of the American Recovery and Reinvestment Act, the ordering, shipping, installation, maintenance and daily use of this microscope will directly stimulate multiple segments of the U.S. economy, and lead to the direct hiring of new technical staff.

描述（由申请人提供）：此S10共享仪器资助申请旨在为约翰霍普金斯大学斑马鱼功能研究中心（也称为FinZ中心）的共聚焦实时成像资源提供资金支持。FinZ中心成立于2008年，由医学院，细胞工程研究所（ICE）和约翰霍普金斯McKusick-Nathans遗传医学研究所（IGM）联合资助。该中心目前由三位科学联合主任Steven Leach，Andrew McCallion和Michael Parsons领导，他们都是斑马鱼遗传学和斑马鱼发育生物学的着名研究人员。连同约书亚门德尔博士，另一个IGM教师从事斑马鱼的工作，这个小组将包括主要用户组提出的蔡司LSM 710共聚焦显微镜。这四个主要用户都是在各种科学领域的高度重视的研究人员，对人类健康有直接影响，包括胰腺发育，胰腺癌，神经元分化，b细胞再生，基因调控序列的计算预测，神经嵴生物学和microRNA在人类恶性肿瘤中的作用。他们共同担任七个NIH R01或P01赠款的PI或co-PI，由四个不同的NIH研究所（NIGM，NICHD，NIDDK和NCI）资助。基于主要用户组对斑马鱼胚胎和其他组织的实时成像的共同需求，以支持其NIH资助的工作，该申请寻求资助蔡司LSM 710 3通道光谱共聚焦系统，该系统具有环境控制系统，以支持斑马鱼胚胎，组织和细胞的长期成像。这一要求的理由是基于以下文件记载的要素：1）蔡司710系统提供了前所未有的信噪比与独特的扫描头设计，提供了机会，应用高分辨率光谱成像到深层斑马鱼组织; 2）事实上，约翰霍普金斯显微镜核心资源在约翰霍普金斯这里已经完全订阅，因此不能容纳10-36小时延时成像协议所需的主要用户组;和3）符合更广泛的目标，美国复苏和再投资法案，订购，运输，安装，维护和日常使用这种显微镜将直接刺激多个部门的美国经济，并导致直接雇用新的技术人员。