Automated high-throughput analysis system

自动化高通量分析系统

• 批准号: 7795052
• 负责人: Satchidananda Panda
• 金额: $ 37.44万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795052
• 关键词: Biological; Biological Assay; Biological Process; Calcium; Cells; Complement; Complementary DNA; DNA; DNA purification; Devices; Dyes; Ensure; Fluorescence; Fluorescence Polarization; Funding; Gene Proteins; Genomics; Human Resources; Institutes; Laboratories; Liquid substance; Mammalian Cell; Measures; Membrane Potentials; Molecular; Preparation; Reader; Reagent; Reproducibility; Research Personnel; Robotics; Sampling; Screening procedure; Speed; System; Systems Analysis; Time; Training; assay development; base; cDNA Library; detector; flexibility; follow-up; high throughput analysis; in vitro Assay; instrument; luminescence; particle; protein function; public health relevance; small hairpin RNA; small molecule libraries

DESCRIPTION (provided by applicant): The proposal is to obtain funds for an automated high throughput analysis system for the instruments core at the Salk Institute. The instrument system will comprise of an Eppendorf epMotion 5075 VAC-TMX high throughput DNA preparation workstation, an Eppendorf epMotion 5075 LH liquid handling station for liquid dispensing and assay set up, and a Molecular Devices FlexStation 3 multi- mode detector for various cell based and in vitro assays. The requested high throughput analyses system is needed to complement the strong chemical library screening core at the Salk Institute. We will use this system to generate and use libraries of cDNA and shRNA reagents for genomics screening. The high throughput genomic DNA purification system will allow preparation of libraries of cDNA or shDNA constructs for cell based assays. The liquid handling station will dispense the cDNA, shDNA and lentiviral particles into mammalian cells. The multimode detector plate reader will measure absorbance, fluorescence intensity, luminescence, fluorescence polarization and time-resolved fluorescence. The Flexstation 3 plate reader will enable researchers at the Salk Institute to use fluorescence based dyes to measure rapid changes in intracellular calcium level or of membrane potential in a high throughput manner. The combination of these instruments will offer a powerful yet flexible platform for use of genomics approaches to accelerate understanding of biological processes in multiple diverse laboratories. We have identified 10 user laboratories for immediate use of the system for various assay development, screening and post-screening follow-up. The proposed instrument system will be integrated to the existing Salk Institute instrument cores. A core instrument use committee will ensure that the instrument system is operated and maintained by trained personnel. PUBLIC HEALTH RELEVANCE: The proposal is to obtain funds for a robotic system at the Salk Institute. This instrument system will enable researchers to screen through thousands of biological samples with high degree of accuracy, reproducibility and speed to understand how genes and proteins function in healthy and diseased cells.

描述（由申请人提供）：该提案是为了获得资金，用于索尔克研究所仪器核心的自动化高通量分析系统。仪器系统将包括Eppendorf epMotion 5075 VAC-TMX高通量DNA制备工作站、用于液体分配和测定设置的Eppendorf epMotion 5075 LH液体处理站以及用于各种基于细胞和体外测定的Molecular Devices FlexStation 3多模式检测器。所要求的高通量分析系统是必要的，以补充强大的化学图书馆筛选在索尔克研究所的核心。我们将使用这个系统来产生和使用cDNA文库和shRNA试剂进行基因组学筛选。高通量基因组DNA纯化系统将允许制备用于基于细胞的测定的cDNA或shDNA构建体的文库。液体处理站将cDNA、shDNA和慢病毒颗粒分配到哺乳动物细胞中。多模式检测板读数器将测量吸光度、荧光强度、发光、荧光偏振和时间分辨荧光。Flexstation 3酶标仪将使索尔克研究所的研究人员能够使用基于荧光的染料以高通量方式测量细胞内钙水平或膜电位的快速变化。这些仪器的结合将为使用基因组学方法提供一个强大而灵活的平台，以加速对多个不同实验室中生物过程的理解。我们已经确定了10个用户实验室，可立即使用该系统进行各种检测开发、筛查和筛查后随访。拟议的仪器系统将被集成到现有的索尔克研究所仪器核心。核心仪器使用委员会将确保仪器系统由经过培训的人员操作和维护。 公共卫生相关性：该提案旨在为索尔克研究所的机器人系统获得资金。该仪器系统将使研究人员能够以高度的准确性，可重复性和速度筛选数千个生物样品，以了解基因和蛋白质在健康和患病细胞中的功能。