Multiphoton Laser Scanning Microscope

描述(由申请人提供)：西奈山医学院的八个主要用户需要一个多光子激光扫描显微镜。该仪器将用于各种器官和组织制剂的多通道荧光和二次谐波成像，包括体外和体内观察。这些研究人员来自基因和细胞医学、免疫生物学、医学、神经病学、神经外科和整形外科，将研究动脉粥样硬化斑块、艾滋病毒相关性肾病、肿瘤转移、肌腱损伤和修复、蛛网膜下腔出血、前庭感觉器官、免疫系统动力学和肾上皮细胞功能。该仪器将位于显微镜共享资源设施内并由其管理，这是一个持续且成功的机构范围内的核心，容纳了其他共焦和宽视场显微镜，目前由来自西奈山100多个实验室的人员使用，该设施的两名全职生物医学工程师工作人员将为所请求的仪器的用户提供监督和培训，并负责其维护。

DESCRIPTION (provided by applicant): A multiphoton laser scanning microscope is requested for the use of eight major users at the Mount Sinai School of Medicine. The instrument will be used for multichannel fluorescence and second harmonic generation imaging of a variety of organs and tissue preparations including both ex-vivo and in-vivo observations. The investigators, from the Departments of Gene and Cell Medicine, Immunobiology, Medicine, Neurology, Neurosurgery and Orthopedic Surgery, will study atherosclerotic plaques, HIV-associated nephropathy, tumor metastasis, tendon damage and repair, subarachnoid hemorrhage, vestibular sensory organs, immune system dynamics, and renal epithelial cell function. The instrument will be situated in and managed by the Microscopy Shared Resource Facility, an ongoing and successful institution-wide core which houses other confocal and widefield microscopes and is currently used by personnel from more than 100 Mount Sinai laboratories, The facility's staff of two full time biomedical engineers will provide supervision and training for users of the requested instrument and will be responsible for its maintenance.