Microscopy

摘要-显微镜共享资源 共享资源领导者：Victor Friedrich博士 Tisch癌症研究所(TCI)显微共享资源设施(MSRF)提供设备、培训、 为各种光学显微镜应用提供专家咨询服务，包括共焦和 多光子显微镜、四个表观荧光/明场系统，包括结构照明 附件、用于反卷积和其他图像处理的软件和工作站以及图像 分析。两名全职、硕士级别的工程师在正常工作时间内在工厂提供真正的 为新手用户提供时间帮助。显微镜系统的高z分辨率和多通道能力 对于涉及免疫荧光和荧光蛋白应用的癌症研究至关重要(例如 细胞信号和生长调节分子的定位，细胞迁移，以及遗传和 对分化的药理操作)。MSRF由机构显微镜组成 共享资源设施和两个TCI卫星站点，以更好地为 蒂施癌症研究所。

ABSTRACT – MICROSCOPY SHARED RESOURCE Shared Resource Leader: Victor Friedrich, PhD The Tisch Cancer Institute (TCI) Microscopy Shared Resource Facility (MSRF) provides equipment, training, and expert consultation services for a full range of light microscopy applications, including confocal and multiphoton microscopes, four epi-fluorescence/brightfield systems, including structured illumination attachments, and software and workstations for deconvolution and other image processing, as well as image analysis. Two full time, masters-level engineers are present in the facility during regular hours to provide real time assistance to novice users. The high z-resolution and multichannel capabilities of the microscope systems are crucial for cancer studies involving immunofluorescence and fluorescent protein applications (e.g. localization of cell signaling and growth regulatory molecules, cell migration, and the effects of genetic and pharmacological manipulations on differentiation). The MSRF is comprised of the institutional Microscopy Shared Resource Facility and two TCI satellite sites that have been set up to better serve members of the Tisch Cancer Institute.