Microscopy

显微镜检查

• 批准号: 9322452
• 负责人: VICTOR Louis FRIEDRICH
• 金额: $ 14.41万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9322452
• 关键词: Calcium; Cancer Center Support Grant; Cell Count; Cell Growth Processes; Cells; Computer software; Consult; Consultations; Doctor of Philosophy; Engineering; Fluorescence; Fluorescence Resonance Energy Transfer; Genetic; Hour; Image; Image Analysis; Immunofluorescence Immunologic; Institutes; Light; Light Microscope; Lighting; Malignant Neoplasms; Measures; Microscope; Microscopy; Modality; Pharmacology; Productivity; Proteins; Protocols documentation; Quantitative Microscopy; Research Personnel; Resolution; Resource Sharing; Services; Signal Transduction; Site; Structure; System; Techniques; Time; Training; cell growth; cell motility; cost; design; equipment training; image processing; instrument; intravital imaging; light microscopy; member; microscopic imaging; operation; particle; protein expression

ABSTRACT – MICROSCOPY SHARED RESOURCE Shared Resource Leader: Victor Friedrich, PhD The Tisch Cancer Institute (TCI) Microscopy Shared Resource Facility (MSRF) provides equipment, training, and expert consultation services for a full range of light microscopy applications, including confocal and multiphoton microscopes, four epi-fluorescence/brightfield systems, including structured illumination attachments, and software and workstations for deconvolution and other image processing, as well as image analysis. Two full time, masters-level engineers are present in the facility during regular hours to provide real time assistance to novice users. The high z-resolution and multichannel capabilities of the microscope systems are crucial for cancer studies involving immunofluorescence and fluorescent protein applications (e.g. localization of cell signaling and growth regulatory molecules, cell migration, and the effects of genetic and pharmacological manipulations on differentiation). The MSRF is comprised of the institutional Microscopy Shared Resource Facility and two TCI satellite sites that have been set up to better serve members of the Tisch Cancer Institute.

摘要 – 显微镜共享资源 共享资源负责人：Victor Friedrich 博士 蒂施癌症研究所 (TCI) 显微镜共享资源设施 (MSRF) 提供设备、培训、 以及全方位光学显微镜应用的专家咨询服务，包括共焦和 多光子显微镜、四个落射荧光/明场系统，包括结构照明 用于反卷积和其他图像处理的附件、软件和工作站，以及图像 分析。两名全职硕士级工程师在正常工作时间内在设施中提供真实的 为新手用户提供时间帮助。显微镜系统的高 z 分辨率和多通道功能 对于涉及免疫荧光和荧光蛋白应用的癌症研究至关重要（例如 细胞信号传导和生长调节分子的定位、细胞迁移以及遗传和细胞因子的影响 分化的药理学操作）。 MSRF 由机构显微镜组成 共享资源设施和两个 TCI 卫星站点的建立是为了更好地为 TCI 成员提供服务 蒂施癌症研究所。