CELLULAR EVENTS IN MYELINATION

髓鞘形成中的细胞事件

• 批准号: 3397467
• 负责人: VICTOR Louis FRIEDRICH
• 金额: $ 7.1万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397467
• 关键词: autoradiography; cell differentiation; electron microscopy; histochemistry /cytochemistry; myelin; myelination; oligodendroglia

Myelin sheaths, elaborated in the central nervous system by oligodendrocytes, are intimately associated with the processes of neurons and act to facilitate the conduction of nerve impulses. The formation of myelin is a prominent part of the development of the nervous system, and the disruption of myelin in human neuropathological conditions such as multiple sclerosis is associated with dramatic deterioration of function. The studies proposed here are aimed at understanding the cellular mechanisms which contribute to the formation of myelin during development, and to its reformation during repair from demyelinating disease. The formation of oligodendrocytes from proliferating precursor cells is necessarily the first step in myelin formation in the central nervous system; however, the precursor cell is poorly understood. These studies will probe the biochemical indentity of the precursor cell by a combination of thymidine autoradiography and immunocytochemical staining, to determine whether the proliferating oligodendrocyte precursor cells contain the myelin specific protiens, myelin basic protein and myelin-associated glycoprotein and, if they do not, at what time after cessation of cell division these proteins appear in the developing oligodendrocytes. Furthermore, changes in immunoreactivity for myelin basic protein and myelin-associated glycoprotein will be correlated with changes in cell fine structure. The production of neuroglial cells will also be studied in two genetic disorders of mouse, quaking and shiverer, which produce excess numbers of oligodendrocytes. Study of this overproduction may give clues to the mechanisms which stimulate oligodendrocyte formation in normal development and in remyelination. The analysis proposed will establish the period of development during which the excess cells are formed in the mutants, using quantitative light and electron microscopy. Gliogenesis in the mutants wil then be examined by thymidine autoradiography and immunocytochemistry, to discover whether the overproduction of oligodendrocytes is associated with stem cells with characteristics similar to or different from those established for normal development.

髓鞘，在中枢神经系统由

项目成果

Oligodendrocyte production and myelin recovery in heterozygous jimpy mice: an autoradiographic study.

杂合子 jimpy 小鼠少突胶质细胞的产生和髓磷脂恢复：放射自显影研究。

• DOI: 10.1016/0736-5748(86)90043-2
• 发表时间: 1986
• 期刊: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
• 作者: Rosenfeld,J;FriedrichJr,VL
• 通讯作者: FriedrichJr,VL

Axonal swellings in jimpy mice: does lack of myelin cause neuronal abnormalities?

jimpy 小鼠的轴突肿胀：髓磷脂缺乏是否会导致神经元异常？

• DOI: 10.1016/0306-4522(83)90233-6
• 发表时间: 1983
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Rosenfeld,J;FreidrichJr,VL
• 通讯作者: FreidrichJr,VL

Altered brain copper and zinc content in quaking mice.

改变了颤抖小鼠的脑铜和锌含量。

• DOI: 10.1016/0014-4886(83)90242-x
• 发表时间: 1983
• 期刊: Experimental neurology
• 影响因子: 5.3
• 作者: Rosenfeld,J;Zimmerman,AW;FriedrichJr,VL
• 通讯作者: FriedrichJr,VL