Quadrupole time-of-flight mass spectrometer

四极杆飞行时间质谱仪

• 批准号: 7793815
• 负责人: AKHILESH PANDEY
• 金额: $ 49.53万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793815
• 关键词: 7 year old; Address; Biological; Biological Markers; Core Facility; Data; Funding; Human Resources; Ions; Knowledge; Laboratories; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Output; Parents; Peptides; Phase; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteome; Proteomics; Public Health Schools; Relative (related person); Request for Proposals; Research; Research Personnel; Resolution; Scanning; Screening for cancer; Techniques; Time; Universities; Validation; base; design; instrument; mass spectrometer; medical schools; multiple reaction monitoring; operation; protein complex; public health relevance; research study

DESCRIPTION (provided by applicant): This proposal requests funds for a high accuracy quadrupole time-of-flight (QTOF) mass spectrometer with high resolution, high sensitivity and fast duty cycle to assist a productive group of investigators at Johns Hopkins University School of Medicine and School of Public Health along with their collaborators in their proteomic studies. This instrument will mainly be used for qualitative studies such as protein identification and analysis of certain post-translational modifications (PTMs) and quantitative studies for measurement of relative abundance of proteins and PTMs. Two existing QTOF instruments (one in the proteomics core facility and one in the Pandey laboratory) are being retired at Johns Hopkins in 2009 as they are old instruments (10 and 7 years old, respectively) and lack many of the advanced features of newer instruments such as fast duty cycles, high mass accuracy, large dynamic range and accurate quantitation capabilities. Owing to this lack of availability of a high resolution tandem mass spectrometer for routine LC-MS/MS type analyses for protein identification and quantitation, the proposed QTOF mass spectrometer is expected to become a major workhorse for the projects in which a team of expert mass spectrometrists and biologists will be greatly benefited. The research groups from the laboratories of Gerald Hart, Philip Cole, Robert Cotter, Bert Vogelstein, Gregg Semenza, Curt Civin, Harold Varmus and Thomas Kensler will be among the major users. Personnel from Akhilesh Pandey's laboratory, along with personnel from Cotter and Hart laboratories, will provide expertise in design of mass spectrometry experiments and LC-MS/MS analysis for those major and other users who do not have mass spectrometry expertise. The advanced features of the QTOF mass spectrometer will be used for iTRAQ and SILAC-based mass spectrometric analysis, among other uses. These quantitative techniques have become very popular and have increased our knowledge about the dynamic changes in proteomes. The biological questions that will be addressed using this mass spectrometry will range from discovery of biomarkers for early detection of cancers and identification of components of protein complexes to studying effects of anti-cancer compounds. Finally, the output of some of the experiments carried out using the proposed QTOF mass spectrometer will be helpful for designing validation experiments using targeted proteomics (e.g. multiple reaction monitoring) on other types of instruments such as triple quadrupole mass spectrometers. PUBLIC HEALTH RELEVANCE: QTOF mass spectrometer is a high resolution mass spectrometer with high sensitivity that allows robust analysis of proteins, peptides and post-translational modifications. It can be used in several modes of operation including data dependent acquisition; parent ion and neutral loss scan modes. This instrument will be essential for the discovery phase and provide essential information about the peptide and proteins to a group of productive biomedical investigators at Johns Hopkins University and their collaborators.

描述（由申请人提供）：该提案要求为高精度四极杆飞行时间（QTOF）质谱仪提供资金，该质谱仪具有高分辨率、高灵敏度和快速占空比，以协助约翰霍普金斯大学医学院和公共卫生学院的富有成效的研究小组及其合作者沿着进行蛋白质组学研究。该仪器将主要用于定性研究，如蛋白质鉴定和某些翻译后修饰（PTM）的分析，以及用于测量蛋白质和PTM相对丰度的定量研究。两台现有的QTOF仪器（一台在蛋白质组学核心设施，一台在Pandey实验室）将于2009年在约翰霍普金斯退役，因为它们是旧仪器（分别为10年和7年），缺乏新仪器的许多先进功能，如快速占空比，高质量精度，大动态范围和准确的定量能力。由于缺乏用于蛋白质鉴定和定量的常规LC-MS/MS类型分析的高分辨率串联质谱仪，拟议的QTOF质谱仪预计将成为项目的主要主力，其中专家质谱师和生物学家团队将大大受益。来自Gerald哈特、Philip科尔、Robert Cotter、Bert Vogelstein、Gregg Semenza、Curt Civin、Harold Varmus和托马斯Kensler实验室的研究小组将成为主要用户。来自Akhilesh Pandey实验室的人员，沿着来自Cotter和哈特实验室的人员，将为那些没有质谱专业知识的主要用户和其他用户提供质谱实验设计和LC-MS/MS分析方面的专业知识。QTOF质谱仪的先进功能将用于iTRAQ和基于SILAC的质谱分析等用途。这些定量技术已经变得非常流行，并增加了我们对蛋白质组动态变化的了解。将使用这种质谱法解决的生物学问题将从发现用于早期检测癌症的生物标志物和鉴定蛋白质复合物的组分到研究抗癌化合物的作用。最后，使用拟议的QTOF质谱仪进行的一些实验的输出将有助于在其他类型的仪器（例如三重四极杆质谱仪）上使用靶向蛋白质组学（例如多反应监测）设计验证实验。 公共卫生相关性：QTOF质谱仪是一种高灵敏度的高分辨率质谱仪，可用于蛋白质、肽和翻译后修饰的稳健分析。它可用于多种操作模式，包括数据相关采集;母离子和中性丢失扫描模式。该仪器将是必不可少的发现阶段，并提供有关肽和蛋白质的基本信息，一组生产生物医学研究人员在约翰霍普金斯大学和他们的合作者。