Quadrupole time-of-flight mass spectrometer

四极杆飞行时间质谱仪

• 批准号: 7793815
• 负责人: AKHILESH PANDEY
• 金额: $ 49.53万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793815
• 关键词: 7 year old; Address; Biological; Biological Markers; Core Facility; Data; Funding; Human Resources; Ions; Knowledge; Laboratories; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Output; Parents; Peptides; Phase; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteome; Proteomics; Public Health Schools; Relative (related person); Request for Proposals; Research; Research Personnel; Resolution; Scanning; Screening for cancer; Techniques; Time; Universities; Validation; base; design; instrument; mass spectrometer; medical schools; multiple reaction monitoring; operation; protein complex; public health relevance; research study

DESCRIPTION (provided by applicant): This proposal requests funds for a high accuracy quadrupole time-of-flight (QTOF) mass spectrometer with high resolution, high sensitivity and fast duty cycle to assist a productive group of investigators at Johns Hopkins University School of Medicine and School of Public Health along with their collaborators in their proteomic studies. This instrument will mainly be used for qualitative studies such as protein identification and analysis of certain post-translational modifications (PTMs) and quantitative studies for measurement of relative abundance of proteins and PTMs. Two existing QTOF instruments (one in the proteomics core facility and one in the Pandey laboratory) are being retired at Johns Hopkins in 2009 as they are old instruments (10 and 7 years old, respectively) and lack many of the advanced features of newer instruments such as fast duty cycles, high mass accuracy, large dynamic range and accurate quantitation capabilities. Owing to this lack of availability of a high resolution tandem mass spectrometer for routine LC-MS/MS type analyses for protein identification and quantitation, the proposed QTOF mass spectrometer is expected to become a major workhorse for the projects in which a team of expert mass spectrometrists and biologists will be greatly benefited. The research groups from the laboratories of Gerald Hart, Philip Cole, Robert Cotter, Bert Vogelstein, Gregg Semenza, Curt Civin, Harold Varmus and Thomas Kensler will be among the major users. Personnel from Akhilesh Pandey's laboratory, along with personnel from Cotter and Hart laboratories, will provide expertise in design of mass spectrometry experiments and LC-MS/MS analysis for those major and other users who do not have mass spectrometry expertise. The advanced features of the QTOF mass spectrometer will be used for iTRAQ and SILAC-based mass spectrometric analysis, among other uses. These quantitative techniques have become very popular and have increased our knowledge about the dynamic changes in proteomes. The biological questions that will be addressed using this mass spectrometry will range from discovery of biomarkers for early detection of cancers and identification of components of protein complexes to studying effects of anti-cancer compounds. Finally, the output of some of the experiments carried out using the proposed QTOF mass spectrometer will be helpful for designing validation experiments using targeted proteomics (e.g. multiple reaction monitoring) on other types of instruments such as triple quadrupole mass spectrometers. PUBLIC HEALTH RELEVANCE: QTOF mass spectrometer is a high resolution mass spectrometer with high sensitivity that allows robust analysis of proteins, peptides and post-translational modifications. It can be used in several modes of operation including data dependent acquisition; parent ion and neutral loss scan modes. This instrument will be essential for the discovery phase and provide essential information about the peptide and proteins to a group of productive biomedical investigators at Johns Hopkins University and their collaborators.

描述（由申请人提供）：该提案请求资金购买具有高分辨率、高灵敏度和快速工作周期的高精度四极杆飞行时间 (QTOF) 质谱仪，以协助约翰·霍普金斯大学医学院和公共卫生学院的一组富有成效的研究人员及其合作者进行蛋白质组研究。该仪器主要用于定性研究，例如蛋白质鉴定和某些翻译后修饰（PTM）的分析，以及定量研究，用于测量蛋白质和PTM的相对丰度。约翰·霍普金斯大学现有的两台 QTOF 仪器（一台位于蛋白质组学核心设施，一台位于 Pandey 实验室）将于 2009 年退役，因为它们是旧仪器（分别有 10 年和 7 年的历史），并且缺乏新仪器的许多先进功能，例如快速占空比、高质量精度、大动态范围和精确定量能力。由于缺乏用于蛋白质鉴定和定量的常规 LC-MS/MS 类型分析的高分辨率串联质谱仪，所提出的 QTOF 质谱仪预计将成为项目的主要主力，在这些项目中，质谱专家和生物学家团队将受益匪浅。来自 Gerald Hart、Philip Cole、Robert Cotter、Bert Vogelstein、Gregg Semenza、Curt Civin、Harold Varmus 和 Thomas Kensler 实验室的研究小组将成为主要用户。 Akhilesh Pandey 实验室的人员以及 Cotter 和 Hart 实验室的人员将为那些不具备质谱专业知识的主要用户和其他用户提供质谱实验设计和 LC-MS/MS 分析方面的专业知识。 QTOF 质谱仪的先进功能将用于 iTRAQ 和基于 SILAC 的质谱分析等用途。这些定量技术已经变得非常流行，并增加了我们对蛋白质组动态变化的了解。使用这种质谱法将解决的生物学问题包括从发现用于早期检测癌症的生物标志物和鉴定蛋白质复合物的成分到研究抗癌化合物的作用。最后，使用所提出的 QTOF 质谱仪进行的一些实验的输出将有助于在其他类型的仪器（例如三重四极杆质谱仪）上使用目标蛋白质组学（例如多反应监测）设计验证实验。 公共健康相关性：QTOF 质谱仪是一种具有高灵敏度的高分辨率质谱仪，可对蛋白质、肽和翻译后修饰进行稳健分析。它可用于多种操作模式，包括数据相关采集；母离子和中性丢失扫描模式。该仪器对于发现阶段至关重要，可为约翰霍普金斯大学的一组富有成效的生物医学研究人员及其合作者提供有关肽和蛋白质的基本信息。