Laser Scanning Confocal Microscope

激光扫描共焦显微镜

• 批准号: 7794252
• 负责人: Donna M Fekete
• 金额: $ 49.96万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794252
• 关键词: Alphavirus; Arts; Axonal Transport; Biological Process; Biological Sciences; Biomedical Research; Blindness; Cell Lineage; Cellular biology; Complement; Cytoskeleton; Defect; Development; Diarrhea; Ear; Eye; Flavivirus; Funding; Gap Junctions; Genes; Growth; Growth Cones; Human; Image; Investments; Lasers; Life; Maintenance; Malignant neoplasm of pancreas; Methods; Microscope; Mitochondria; Nervous system structure; Neurodegenerative Disorders; Pain; Perception; Photoreceptors; Productivity; Purdue Cancer Center; Regulation; Reporting; Research; Research Infrastructure; Research Personnel; Salmonella; Sampling; Scanning; Sensory; TRP channel; Training; Universities; cancer initiation; career; cell motility; cellular imaging; deafness; imaging modality; instrumentation; neuronal growth; pathogen; repaired; sound; success; tool

DESCRIPTION (provided by applicant): Nineteen researchers in the Department of Biological Sciences and the Purdue Cancer Center at Purdue University seek support for advanced live cell imaging with a Zeiss LSM 710 confocal microscope on an inverted microscope platform equipped with an incubation chamber. Live cell imaging is at the forefront modern cell biology and lack of access to these methods cuts off Purdue investigators from key advances. Support for this microscope provides essential research infrastructure to a successful cadre of NIH-funded researchers with a sound record of productivity, reporting significant advances in biomedical research. Acquisition of this microscope by this user group will open new research avenues for proven researchers who are poised to move strongly and successfully into advanced live cell imaging using the LSM 710. A sampling of the NIH-funded research that purchase of this microscope will support includes: the development of the vertebrate eye and ear in an effort to discover new genes underlying blindness (Leung) or deafness (Fekete) in humans; the mechanism by which defects in axonal transport of mitochondria give rise to neurodegenerative disease (Hollenbeck); cell lineages involved in pancreatic cancer initiation (Konieczny); cellular details of replication and assembly of human pathogen alphaviruses and flaviviruses (Kuhn); the function of TRP channels that are important in a vast array of biological functions, including sensory perception, especially pain (Pak); development and maintenance of healthy photoreceptors (Chang, Ready), regulation of neuronal growth cone motility and guidance critical to nervous system growth and repair (Suter); how Salmonella exploits the host acting cytoskeleton network to promote Salmonella entry and induce diarrhea in humans (Zhou). The requested inverted microscope complements the recent acquisition of an upright Zeiss LSM 710 by the Purdue Life Sciences Fluorescent Imaging Facility. Having both platforms will allow Purdue investigators to move seamlessly between upright and inverted microscopes so as to choose the best tool for each enquiry. This will also provide economies in training, administration and support. The microscope will be a nexus of interdisciplinary exchange of ideas and methods. In addition to serving established investigators, it will provide a cadre of outstanding young researcher's state of the art instrumentation at the critical outset of their careers. Lack of advanced live cell imaging methods is a significant research bottleneck at Purdue and investment in this microscope will stimulate experimental and intellectual success in biomedical research.

描述（由申请人提供）：普渡大学生物科学系和普渡癌症中心的 19 名研究人员寻求在配备孵化室的倒置显微镜平台上使用蔡司 LSM 710 共焦显微镜进行高级活细胞成像的支持。活细胞成像处于现代细胞生物学的最前沿，缺乏这些方法使普渡大学的研究人员无法取得关键进展。对这款显微镜的支持为 NIH 资助的一批成功的研究人员提供了必要的研究基础设施，这些研究人员具有良好的生产力记录，报告了生物医学研究的重大进展。该用户组购买这款显微镜将为经过验证的研究人员开辟新的研究途径，他们准备使用 LSM 710 强有力地成功地进入先进的活细胞成像领域。购买这款显微镜将支持的 NIH 资助研究样本包括： 脊椎动物眼睛和耳朵的发育，以发现人类失明 (Leung) 或耳聋 (Fekete) 的新基因；线粒体轴突运输缺陷引起神经退行性疾病的机制（Hollenbeck）；参与胰腺癌发生的细胞谱系 (Konieczny)；人类病原体甲病毒和黄病毒复制和组装的细胞细节（Kuhn）； TRP 通道的功能对于许多生物功能都很重要，包括感觉知觉，尤其是疼痛 (Pak)；健康感光器的发育和维护（Chang，Ready），神经元生长锥运动的调节以及对神经系统生长和修复至关重要的指导（Suter）；沙门氏菌如何利用宿主细胞骨架网络促进沙门氏菌进入并诱发人类腹泻（Zhou）。 所要求的倒置显微镜是对普渡大学生命科学荧光成像设施最近购买的直立蔡司 LSM 710 的补充。拥有这两个平台将使普渡大学的研究人员能够在正置显微镜和倒置显微镜之间无缝切换，以便为每次调查选择最佳工具。这也将节省培训、管理和支持方面的费用。显微镜将成为跨学科思想和方法交流的纽带。除了为成熟的研究人员提供服务外，它还将为一批杰出的年轻研究人员在其职业生涯的关键初期提供最先进的仪器。缺乏先进的活细胞成像方法是普渡大学的一个重大研究瓶颈，对这款显微镜的投资将刺激生物医学研究的实验和智力成功。