Laser Scanning Confocal Microscope

描述（由申请人提供）：普渡大学生物科学系和普渡癌症中心的19名研究人员寻求支持，在配备孵育室的倒置显微镜平台上使用蔡司LSM 710共聚焦显微镜进行先进的活细胞成像。活细胞成像是现代细胞生物学的前沿，缺乏这些方法切断了普渡大学研究人员的关键进展。对这种显微镜的支持为NIH资助的研究人员的成功骨干提供了必要的研究基础设施，这些研究人员具有良好的生产力记录，报告了生物医学研究的重大进展。该用户组购买该显微镜将为那些准备使用LSM 710强大而成功地进入先进活细胞成像的成熟研究人员开辟新的研究途径。 购买这台显微镜将支持NIH资助的研究样本包括：脊椎动物眼睛和耳朵的发展，以努力发现人类失明（Leung）或耳聋（Fekete）的新基因;线粒体轴突运输缺陷引起神经退行性疾病的机制（Hollenbeck）;参与胰腺癌起始的细胞谱系人病原体甲病毒和黄病毒复制和组装的细胞细节（Kuhn）; TRP通道的功能，其在大量生物功能中是重要的，包括感官知觉，特别是疼痛（Pak）;发展和维持健康的光感受器（Chang，Ready），神经生长锥运动的调节和对神经系统生长和修复至关重要的指导（Suter）;沙门氏菌如何利用宿主细胞骨架网络促进沙门氏菌进入并诱导人类腹泻（Zhou）。 所要求的倒置显微镜补充了普渡生命科学荧光成像设施最近收购的一个直立蔡司LSM 710。拥有这两个平台将使普渡大学的研究人员能够在直立和倒置显微镜之间无缝移动，以便为每次调查选择最佳工具。这也将节省培训、行政和支助费用。显微镜将成为跨学科思想和方法交流的纽带。除了为既定的调查人员服务外，它还将在他们职业生涯的关键开端提供一批优秀的年轻研究人员的最先进仪器。缺乏先进的活细胞成像方法是普渡大学的一个重要研究瓶颈，对这种显微镜的投资将刺激生物医学研究的实验和智力成功。

DESCRIPTION (provided by applicant): Nineteen researchers in the Department of Biological Sciences and the Purdue Cancer Center at Purdue University seek support for advanced live cell imaging with a Zeiss LSM 710 confocal microscope on an inverted microscope platform equipped with an incubation chamber. Live cell imaging is at the forefront modern cell biology and lack of access to these methods cuts off Purdue investigators from key advances. Support for this microscope provides essential research infrastructure to a successful cadre of NIH-funded researchers with a sound record of productivity, reporting significant advances in biomedical research. Acquisition of this microscope by this user group will open new research avenues for proven researchers who are poised to move strongly and successfully into advanced live cell imaging using the LSM 710. A sampling of the NIH-funded research that purchase of this microscope will support includes: the development of the vertebrate eye and ear in an effort to discover new genes underlying blindness (Leung) or deafness (Fekete) in humans; the mechanism by which defects in axonal transport of mitochondria give rise to neurodegenerative disease (Hollenbeck); cell lineages involved in pancreatic cancer initiation (Konieczny); cellular details of replication and assembly of human pathogen alphaviruses and flaviviruses (Kuhn); the function of TRP channels that are important in a vast array of biological functions, including sensory perception, especially pain (Pak); development and maintenance of healthy photoreceptors (Chang, Ready), regulation of neuronal growth cone motility and guidance critical to nervous system growth and repair (Suter); how Salmonella exploits the host acting cytoskeleton network to promote Salmonella entry and induce diarrhea in humans (Zhou). The requested inverted microscope complements the recent acquisition of an upright Zeiss LSM 710 by the Purdue Life Sciences Fluorescent Imaging Facility. Having both platforms will allow Purdue investigators to move seamlessly between upright and inverted microscopes so as to choose the best tool for each enquiry. This will also provide economies in training, administration and support. The microscope will be a nexus of interdisciplinary exchange of ideas and methods. In addition to serving established investigators, it will provide a cadre of outstanding young researcher's state of the art instrumentation at the critical outset of their careers. Lack of advanced live cell imaging methods is a significant research bottleneck at Purdue and investment in this microscope will stimulate experimental and intellectual success in biomedical research.