A confocal microscope

描述(由申请人提供)：本申请申请购买一个新的单光子多荧光/光谱共聚焦激光扫描显微镜(CLSM)，作为位于新泽西州立大学罗格斯分校库克校区的环境与生物科学学院(SEBS)的几个研究项目的共享资源。具有这些能力的共焦显微镜可以满足目前正在进行的NIH资助项目的需求，库克校园中没有这样的共焦显微镜。为了满足这一需求，我们建议购买一台共焦显微镜，作为库克校园多用户生物成像设备的核心。分布在六个部门的至少九个研究实验室将立即受益于使用这样的设施。生物成像设施将为调查人员提供现代细胞生物成像技术方面的资源和培训，并将加强调查人员之间的跨学科活动。多用户生物成像设施的主要重点将是应用荧光探针作为研究与蛋白质和脂肪运输有关的细胞过程的手段，重点是将高灵敏度和定量的现代光学显微镜技术与分子、生物化学和基因组方法相结合。所要求的蔡司LSM 710的功能包括激发和检测更广泛的荧光探针，用于细胞结构和蛋白质的多色成像，用于研究蛋白质-蛋白质相互作用的荧光共振能量转移(FRET)和钙成像。所要求的共聚焦显微镜可以光谱分离具有重叠发射光谱的多个荧光团，允许用户以非常高的灵敏度可视化多种颜色并去除背景和自体荧光。购买这种共聚焦显微镜将使美国国立卫生研究院资助的库克校区的研究人员能够将他们的研究扩展到新的方向，并对他们的领域做出重要贡献。预计这种新设备将加强罗格斯大学作为生物医学研究领导者的作用，并为NIH资助的教职员工提供获得尖端生物成像方法的途径。

DESCRIPTION (provided by applicant): This application requests funds to purchase a new single photon multi-fluorescence/spectral confocal laser-scanning microscope (CLSM) to be used as a shared resource for several research programs at the School of Environmental and Biological Sciences (SEBS) located on the Cook Campus of Rutgers, the State University of New Jersey. A confocal microscope with these capabilities that can meet the needs of NIH funded projects currently underway does not exist on the Cook Campus. To address this need, we propose to purchase a confocal microscope as the nucleus of a multi-user Biological Imaging Facility on the Cook Campus. At least nine research labs spread across six departments would immediately benefit from access to such a facility. The Biological Imaging Facility will provide investigators resources and training in modern cell biological imaging techniques and will enhance interdisciplinary activities among investigators. The major focus of the multi-user Biological Imaging Facility will be the application of fluorescent probes as a means of investigating cellular processes relevant to protein and lipid transport with emphasis on the integration of highly sensitive and quantitative modern light microscopy techniques with molecular, biochemical and genomic approaches. The capabilities of the requested Zeiss LSM 710 include excitation and detection of a wider range of fluorescent probes for multicolor imaging of cellular structures and proteins, fluorescence resonance energy transfer (FRET) for studies of protein-protein interactions and calcium imaging. The requested confocal microscope can spectrally separate multiple fluorophores with overlapping emission spectra, allowing the user to visualize multiple colors and remove background and autofluorescence with a very high sensitivity. The acquisition of this confocal microscope will enable NIH-funded investigators on the Cook Campus to extend their research in new directions and generate important contributions to their fields. It is expected that this new equipment will strengthen the role of Rutgers University as a leader in biomedical research and provide NIH funded faculty access to cutting edge biological imaging approaches.