Interaction of ricin A chain with the ribosomal stalk
蓖麻毒素 A 链与核糖体柄的相互作用
基本信息
- 批准号:8410079
- 负责人:
- 金额:$ 5.34万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-01-01 至 2015-12-31
- 项目状态:已结题
- 来源:
- 关键词:ApoptosisArchaeaArchitectureBacteriaBindingBiochemicalBiological ModelsCell DeathCellsCollaborationsComplexDataDefectDepurinationDevelopmentDockingEnvironmentGlycine decarboxylaseGrantHumanInduction of ApoptosisLinkMAPK14 geneMAPK8 geneMammalian CellMessenger RNAMethodsModelingMolecularMolecular ProbesMutagenesisMyelin P2 ProteinNatureNew BrunswickNew JerseyParentsPolandPreparationProceduresProtein BiosynthesisProteinsRNA SplicingResearchResistanceResourcesRibosomal ProteinsRibosomal RNARibosomesRicinRicin A ChainRoleSaccharomycesShiga-Like ToxinsSignal PathwaySignal TransductionSiteSmall Interfering RNASpainSpecificityStructureSystemTimeUnited States National Institutes of HealthUniversitiesYeastsbasecytotoxicitygenetic analysisin vivoinsightmutantnovelparent grantpokeweed antiviral proteinpreventtool
项目摘要
PROJECT SUMMARY
This is a FIRCA application for the collaboration between Dr. Nilgun Tumer at Rutgers
University, New Brunswick, New Jersey and Dr. Marek Tch¿rzewski at the Maria Curie
Sklodowska University in Poland. This research will be done at the Maria Curie Sklodowska
University in Poland as an extension of NIH Grant No. R01-AI072425, 3-15-2007 to 2-29-2012.
This collaboration makes use of the unique resources and research environment in Dr.
Tch¿rzewski's lab to use ricin A chain (RTA) as a molecular tool to probe the function of the
eukaryotic ribosomal stalk and to identify the functional part of the stalk structure. In spite of its
fundamental importance in protein synthesis, the molecular architecture of the eukaryotic stalk
and details of its function are not known due to the lack of appropriate molecular tools. Dr.
Tch¿rzewski has developed the yeast strains and the methods to isolate the in vivo assembled
eukaryotic ribosomal stalk for the first time. Isolation of the whole native stalk from yeast has
revolutionized the method of stalk preparation and facilitated research on the function of the
eukaryotic ribosomal stalk. Dr. Tumer has developed the model systems to study the activity of
ricin A chain (RTA) and discovered that RTA binds to the ribosomal stalk to inhibit protein
synthesis. In collaboration with Dr. Tch¿rzewski, Dr. Tumer showed direct interaction between
RTA and the isolated native yeast ribosomal stalk, and obtained preliminary data demonstrating
that RTA can be used as a very specific molecular tool to probe the structure and the function of
the eukaryotic stalk. We propose to identify the stalk components critical for binding RTA, study
the interaction of RTA with stalk complexes from different species, including bacteria, archaea,
yeast and human and to identify the functional part of the stalk structure. This collaboration will
connect the ongoing structure function analysis of RTA in the parent grant with biochemical,
structural and genetic analysis of the stalk in Dr. Tch¿rzewski's lab in Poland and will provide
new insights into the role of the ribosomal stalk in protein synthesis.
项目概要
这是罗格斯大学 Nilgun Tumer 博士合作的 FIRCA 申请
新泽西州新不伦瑞克大学和 Marek Tch¿rzewski 博士在玛丽亚居里
波兰斯克洛多夫斯卡大学。这项研究将在 Maria Curie Sklodowska 进行
波兰大学作为 NIH 拨款的延伸,编号为 R01-AI072425,2007 年 3 月 15 日至 2012 年 2 月 29 日。
此次合作利用了博士的独特资源和研究环境。
Tch¿rzewski 的实验室使用蓖麻毒素 A 链 (RTA) 作为分子工具来探测蓖麻毒素 A 链的功能
真核核糖体茎并鉴定茎结构的功能部分。尽管其
在蛋白质合成、真核生物茎的分子结构中具有根本重要性
由于缺乏适当的分子工具,其功能细节尚不清楚。博士。
Tch¿rzewski 开发了酵母菌株以及分离体内组装的方法
首次发现真核生物核糖体柄。从酵母中分离出整个天然茎
彻底改变了茎的制备方法并促进了对茎的功能的研究
真核生物核糖体柄。 Tumer 博士开发了模型系统来研究
蓖麻毒素 A 链 (RTA) 并发现 RTA 与核糖体柄结合以抑制蛋白质
合成。 Tumer 博士与 Tch¿rzewski 博士合作,展示了
RTA和分离的天然酵母核糖体茎,并获得初步数据证明
RTA可以作为一种非常具体的分子工具来探测结构和功能
真核生物茎。我们建议确定对结合 RTA 至关重要的茎成分,研究
RTA 与不同物种的茎复合物的相互作用,包括细菌、古细菌、
酵母和人类并鉴定茎结构的功能部分。此次合作将
将母基金中正在进行的 RTA 结构功能分析与生化联系起来,
在波兰 Tch¿rzewski 博士的实验室中对茎进行结构和遗传分析,并将提供
关于核糖体茎在蛋白质合成中的作用的新见解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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NILGUN E TUMER其他文献
NILGUN E TUMER的其他文献
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{{ truncateString('NILGUN E TUMER', 18)}}的其他基金
Role of the ribosomal stalk in the activity of Shiga toxins
核糖体柄在志贺毒素活性中的作用
- 批准号:
8432004 - 财政年份:2012
- 资助金额:
$ 5.34万 - 项目类别:
Role of the ribosomal stalk in the activity of Shiga toxins
核糖体柄在志贺毒素活性中的作用
- 批准号:
8303644 - 财政年份:2012
- 资助金额:
$ 5.34万 - 项目类别:
Interaction of ricin A chain with the ribosomal stalk
蓖麻毒素 A 链与核糖体柄的相互作用
- 批准号:
8209110 - 财政年份:2011
- 资助金额:
$ 5.34万 - 项目类别:
Interaction of ricin A chain with the ribosomal stalk
蓖麻毒素 A 链与核糖体柄的相互作用
- 批准号:
7942717 - 财政年份:2011
- 资助金额:
$ 5.34万 - 项目类别:
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