A Zeiss LSM 710 confocal microscope for the Biological Imaging Facility at the U

描述（由申请人提供）：Ruzin这是为CNR生物成像设施（BIF）获得美国国立卫生研究院资助的卡尔蔡司LSM710激光扫描共聚焦显微镜的提案。该显微镜系统将增强BIF的研究和培训能力，特别是将允许本提案中列出的八(8)个主要用户的研究继续进行其资助的研究。对于加州大学伯克利分校的许多研究人员来说，BIF是涉及光学显微镜的生物学研究的起点。然而，一个主要的限制是，由于现有的10年历史的LSM510 META共聚焦显微镜的局限性，无法在FRAP实验中分辨微弱的荧光，和/或使用共聚焦显微镜进行长期延时荧光成像。该仪器将填补加州大学伯克利分校自然资源学院、文理学院和一般科学界在光学仪器方面急需的空白。在过去的一年里，我们对八个生物系统进行了初步实验，比较了使用蔡司LSM710共聚焦显微镜的演示结果和使用BIF的LSM510共聚焦显微镜获得的结果。对于每个项目，我们提供的证据表明，LSM710显微镜远远超过我们现有的蔡司LSM510的灵敏度和分辨率的能力。本提案中描述的研究人员参与了微生物和真核生物事件的无数方面；所有这些都需要更高灵敏度的共聚焦显微镜来充分地观察他们特定的生物学问题。本提案中描述的研究涉及：(1)细菌（Coates, Fleiszig, Koleili, Lindow），果蝇染色体（Botchan），多层哺乳动物腺泡（Luo），作用于T3T细胞的podosome (Martin)，树突中的RNA颗粒（Napoli）和酵母核孔复合体（Weis）的荧光可视化。这些项目共同代表了加州大学伯克利分校生物成像设施所必需的共聚焦成像的广度。

DESCRIPTION (provided by applicant): Ruzin this is a proposal to obtain NIH funding for a Carl Zeiss LSM710 laser scanning confocal microscope for the CNR Biological Imaging Facility (BIF). This microscope system will augment the research and training capabilities of the BIF, and specifically, will permit the research of the eight (8) Major Users listed in this proposal to proceed forward with their funded research. The BIF is the starting point for biological research involving optical microscopy for a great many researchers at UC Berkeley. A major limitation, however, is the inability to resolve dim fluorescence do FRAP experiments, and/or long-term time-lapse fluorescence imaging using confocal microscopy due to the limitations of the existing 10-year old LSM510 META confocal microscope. The requested instrument will fill a much-needed gap in optical instrumentation in the College of Natural Resources, the College of Letters and Sciences, and the general scientific community at the University of California, Berkeley. Over the last year we performed preliminary experiments on eight biological systems, comparing the results obtainable using a demo Zeiss LSM710 confocal microscope to that obtained using the BIF's LSM510 confocal microscope. For each project we present evidence showing that the LSM710 microscope far surpasses the capabilities of our existing Zeiss LSM510 in sensitivity and resolution. The researchers described in this proposal are involved in myriad aspects of microbial and eukaryotic biological events; all of which require higher sensitivity confocal microscopy to adequately visualize their particular biological questions. The research described in this proposal involves fluorescence visualization of: (1) bacteria (Coates, Fleiszig, Koleili, Lindow), Drosophila chromosomes (Botchan), multi-layered mammalian acini (Luo), podosome acting in T3T cells (Martin), RNA granules in dendrites (Napoli), and the yeast nuclear pore complex (Weis). Together these projects represent the breadth of confocal imaging that is necessary for the Biological Imaging Facility at UC Berkeley.