A Zeiss Elyra S1 Structured Illumination Microscope for the Biological Imaging Fa

用于生物成像的 Zeiss Elyra S1 结构照明显微镜

• 批准号: 8640418
• 负责人: Steven Earl Ruzin
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640418
• 关键词: Biological; California; Color; Communities; Funding; Goals; Image; Imaging Techniques; Imaging technology; Letters; Lighting; Microscope; Microscopy; Optics; Research; Research Personnel; Research Project Grants; Research Training; Resolution; Resources; Sampling; Science; Scientist; Structure; System; Time; United States National Institutes of Health; Universities; Work; college; instrument; microbial alkaline proteinase inhibitor; optical imaging; research study

DESCRIPTION (provided by applicant): The purpose of this proposal is to acquire NIH funding for a Carl Zeiss Elyra S1 SIM system for the CNR Biological Imaging Facility (BIF). This super-resolution microscope system will augment the research and training capabilities of the BIF, and specifically, will permit the research goals of the six (6) Major Users listed in this proposal to proceed forward with their active NIH-funded research. The BIF is the main focus for research involving optical microscopy for a great many scientists at UC Berkeley (379 PIs/2431 users). A major limitation, however, is the inability to resolve fluorescent targets at a "super-resolution" level. At the present time the highest resolution optical imaging technique available to these users is deconvolution (API DeltaVision Elite). There is only one super-resolution system (PALM) available on campus. Our researchers require 2-3 channel 3D super-resolution imaging, and thus only a Structured Illumination Microscope (SIM) is appropriate for our needs. STED does not work with long wavelength probes and is therefore disqualified for all of our users. PALM/STORM yields little or no 3D information. In this proposal we have shown that the research of our Major Users is crippled by the lack of the ability to resolve structures smaller than diffraction-limited resolution of 200nm. The requested instrument will fill a much-needed gap in optical imaging technology by providing reproducible 100nm resolution using any color probe. This critical capability will facilitate the research of scientists in the College of Natura Resources, the College of Letters and Sciences, and the general scientific community at the University of California, Berkeley. Over the last year we performed a preliminary SIM experiments on biological samples using a Carl Zeiss Elyra S1, Nikon N-SIM, and the API OMX, and we compared the results to that obtained using the BIF's deconvolution and LSM 710 confocal microscopes. For each Major User's research project we present unequivocal evidence showing that SIM imaging far surpasses the capabilities of our existing microscopes in resolution, sensitivity, and the ability to image in 3D.

描述（由申请人提供）：该提案的目的是为CNR生物成像设施（BIF）的Carl Zeiss Elyra S1 SIMS系统获得NIH资金。这种超分辨率显微镜系统将增强BIF的研究和培训能力，具体来说，将允许本提案中列出的六（6）个主要用户的研究目标，以继续进行其积极的NIH资助研究。 BIF是加州大学伯克利分校（379 PIS/2431用户）的许多科学家的光学显微镜研究的主要重点。但是，一个主要的局限性是无法在“超分辨率”水平上解决荧光目标。目前，这些用户可用的最高分辨率光学成像技术是反卷积（API Deltavision Elite）。校园只有一个超分辨率系统（棕榈）。我们的研究人员需要2-3通道3D超分辨率成像，因此只有结构化照明显微镜（SIM）适合我们的需求。 STED不适用于长波长探测器，因此对于我们所有用户而言都取消了资格。棕榈/暴风雨几乎不会产生3D信息。在这项建议中，我们表明，主要用户的研究缺乏解决小于200nm衍射限制分辨率的结构的能力所削弱。该请求的仪器将通过使用任何颜色探针提供可重现的100NM分辨率来填补光学成像技术中急需的空白。这种关键能力将促进自然资源学院，文书和科学学院以及加利福尼亚大学伯克利分校的一般科学界的科学家研究。在过去的一年中，我们使用Carl Zeiss Elyra S1，Nikon N-SIM和API OMX进行了对生物样品的初步SIM实验，我们将结果与使用BIF的Deonsonvolution和LSM 710共斑显微镜进行了比较。对于每个主要用户的研究项目，我们提供明确的证据，表明SIM成像远远超过了我们现有显微镜在分辨率，敏感性以及在3D中形象的能力方面的功能。

项目成果

Horizontal 'gene drives' harness indigenous bacteria for bioremediation.

水平“基因驱动”利用本土细菌进行生物修复。

• DOI: 10.1038/s41598-020-72138-9
• 发表时间: 2020
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: French,KatherineE;Zhou,Zhongrui;Terry,Norman
• 通讯作者: Terry,Norman

Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging.

• DOI: 10.1091/mbc.e18-07-0424
• 发表时间: 2018-11-01
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Emmerstorfer-Augustin A;Augustin CM;Shams S;Thorner J
• 通讯作者: Thorner J

Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2.

• DOI: 10.1101/gad.318709.118
• 发表时间: 2018-12-01
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Leskoske KL;Roelants FM;Emmerstorfer-Augustin A;Augustin CM;Si EP;Hill JM;Thorner J
• 通讯作者: Thorner J

Subdiffraction-resolution live-cell imaging for visualizing thylakoid membranes.

• DOI: 10.1111/tpj.14021
• 发表时间: 2018-10
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Iwai M;Roth MS;Niyogi KK
• 通讯作者: Niyogi KK

Segregation of four Agrobacterium tumefaciens replicons during polar growth: PopZ and PodJ control segregation of essential replicons

• DOI: 10.1073/pnas.2014371117
• 发表时间: 2020-10
• 期刊: Proceedings of the National Academy of Sciences
• 作者: J. S. Robalino-Espinosa;J. S. Robalino-Espinosa;John R. Zupan;A. Chávez-Arroyo;P. Zambryski