A DeltaVision Restoration Microscope forCNR Imaging

用于 CNR 成像的 DeltaVision 修复显微镜

• 批准号: 6440318
• 负责人: Steven Earl Ruzin
• 金额: $ 25.32万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440318
• 关键词: Agrobacterium; Bacillus subtilis; Caenorhabditis elegans; Drosophilidae; aging; bioimaging /biomedical imaging; biomedical equipment purchase; chromosomes; confocal scanning microscopy; fibroblasts; fluorescence microscopy; insect virus; light microscopy; membrane transport proteins; microscopy; mitochondria; protein localization; protein structure function; transcription factor; virion

DESCRIPTION (provided by applicant): The purpose of this proposal is to acquire NIH funding for a DeltaVision Restoration microscope for the CNR Biological Imaging Facility. This microscope system will augment the research and training capabilities of the Biological Imaging Facility, and specifically, will permit the research goals of the seven Major Users listed in this proposal to proceed forward. The Biological Imaging Facility is the only optical microscope imaging laboratory on campus, and as such is the starting point for biological research involving optical microscopy for a great many researchers at UC Berkeley. A major limitation, however, is the inability to resolve dim fluorescence, or resolution-limited samples. The requested instrument will fill a much-needed gap in optical instrumentation in the College of Natural Resources and the general scientific community at the University of California, Berkeley. Over the last year we performed preliminary experiments on seven biological systems, comparing the results obtainable from use on a DeltaVision deconvolution microscope to that obtained using the Imaging Facility's Zeiss 510 confocal microscope. For each project we present evidence showing that the DeltaVision restoration microscope far surpasses the capabilities of the Zeiss 510 in resolution and sensitivity. As a group, the researchers described in this proposal are involved in myriad aspects of microbial and sub-microbial biological events; all of which require deconvolution microscopy to adequately visualize their particular biological questions. The research described in this proposal involves fluorescence visualization of: (1) regulatory proteins SpoIlR, SpoIIGA, and pro-i in Bacillus subtilis (Hofmeister), (2) GFP-Iabeled AcMNPV virions during infection of insect cell (Volkman), (3) the suboellular localization of VirB proteins and the colocalization of T-Transporter components in Agrobacterium (Zambryski), (4) mitochondria structure changes during aging in fibroblasts (Ames), (5) transcription elongation factors Sdtlp in yeast (Kane), (6) 3D colocalization of dosage-compensation and SMC proteins on the Caenorhabditis elegans pachytene chromosomes (Meyer), and (7) characterization and colocalization of transcription factors in interphase Drosophila melanogaster cells (Tjian).

描述（由申请人提供）：本提案的目的是获得 NIH为CNR生物学研究中心的DeltaVision恢复显微镜提供资金 成像设备。这个显微镜系统将加强研究和培训 生物成像设施的能力，特别是，将允许 本建议书中列出的七个主要用户的研究目标 性新生物成像设备是唯一的光学显微镜成像 校园里的实验室，因此是生物研究的起点 包括光学显微镜，为加州大学伯克利分校的许多研究人员。一 然而，主要的限制是不能分辨暗淡的荧光，或者 分辨率有限的样本。所要求的文书将填补急需的 在自然资源学院光学仪器的差距和 一般科学界在加州大学伯克利分校。 在过去的一年里，我们对七种生物进行了初步实验， 系统，比较在DeltaVision上使用可获得的结果 去卷积显微镜，以获得使用成像设施的蔡司 510共聚焦显微镜。对于每一个项目，我们提出的证据表明， DeltaVision修复显微镜远远超过蔡司的能力 510分辨率和灵敏度。作为一个群体，研究人员在 这个建议涉及微生物和亚微生物的无数方面， 生物事件;所有这些都需要去卷积显微镜来充分 将他们的特定生物学问题形象化。本文中描述的研究 一项提案涉及荧光可视化：（1）调节蛋白 枯草芽孢杆菌（Hofmeister）中的SpoIIR、SpoIIGA和pro-i，（2）GFP标记的 AcMNPV病毒粒子在感染昆虫细胞（B.p.man）过程中， VirB蛋白的定位和T转运蛋白的共定位 （4）线粒体结构的变化 在成纤维细胞老化过程中（艾姆斯），（5）转录延伸因子Sdtlp 在酵母（Kane）中，（6）剂量补偿和SMC蛋白的3D共定位 在秀丽隐杆线虫粗线期染色体上（Meyer），和（7） 间期转录因子的鉴定和共定位 果蝇细胞（Tjian）。