A DeltaVision Restoration Microscope forCNR Imaging

用于 CNR 成像的 DeltaVision 修复显微镜

• 批准号: 6440318
• 负责人: Steven Earl Ruzin
• 金额: $ 25.32万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440318
• 关键词: Agrobacterium; Bacillus subtilis; Caenorhabditis elegans; Drosophilidae; aging; bioimaging /biomedical imaging; biomedical equipment purchase; chromosomes; confocal scanning microscopy; fibroblasts; fluorescence microscopy; insect virus; light microscopy; membrane transport proteins; microscopy; mitochondria; protein localization; protein structure function; transcription factor; virion

DESCRIPTION (provided by applicant): The purpose of this proposal is to acquire NIH funding for a DeltaVision Restoration microscope for the CNR Biological Imaging Facility. This microscope system will augment the research and training capabilities of the Biological Imaging Facility, and specifically, will permit the research goals of the seven Major Users listed in this proposal to proceed forward. The Biological Imaging Facility is the only optical microscope imaging laboratory on campus, and as such is the starting point for biological research involving optical microscopy for a great many researchers at UC Berkeley. A major limitation, however, is the inability to resolve dim fluorescence, or resolution-limited samples. The requested instrument will fill a much-needed gap in optical instrumentation in the College of Natural Resources and the general scientific community at the University of California, Berkeley. Over the last year we performed preliminary experiments on seven biological systems, comparing the results obtainable from use on a DeltaVision deconvolution microscope to that obtained using the Imaging Facility's Zeiss 510 confocal microscope. For each project we present evidence showing that the DeltaVision restoration microscope far surpasses the capabilities of the Zeiss 510 in resolution and sensitivity. As a group, the researchers described in this proposal are involved in myriad aspects of microbial and sub-microbial biological events; all of which require deconvolution microscopy to adequately visualize their particular biological questions. The research described in this proposal involves fluorescence visualization of: (1) regulatory proteins SpoIlR, SpoIIGA, and pro-i in Bacillus subtilis (Hofmeister), (2) GFP-Iabeled AcMNPV virions during infection of insect cell (Volkman), (3) the suboellular localization of VirB proteins and the colocalization of T-Transporter components in Agrobacterium (Zambryski), (4) mitochondria structure changes during aging in fibroblasts (Ames), (5) transcription elongation factors Sdtlp in yeast (Kane), (6) 3D colocalization of dosage-compensation and SMC proteins on the Caenorhabditis elegans pachytene chromosomes (Meyer), and (7) characterization and colocalization of transcription factors in interphase Drosophila melanogaster cells (Tjian).

描述（由申请人提供）：本提案的目的是获取 用于CNR生物学的Deltavision恢复显微镜的NIH资金 成像设施。该显微镜系统将增加研究和培训 生物成像设施的功能，特别是 该提案中列出的七个主要用户的研究目标继续进行 向前。生物成像设施是唯一的光学显微镜成像 校园的实验室，因此是生物学研究的起点 涉及加州大学伯克利分校的许多研究人员的光学显微镜。一个 然而，主要限制是无法解决昏暗的荧光，或 分辨率有限的样品。请求的仪器将填补急需的 自然资源学院的光学仪器差距和 加州大学伯克利分校的一般科学界。 在过去的一年中，我们对七个生物学进行了初步实验 系统，比较可在Deltavision上使用的结果 使用成像设施的Zeiss获得的反卷积显微镜 510共聚焦显微镜。对于每个项目，我们提供证据，表明 三角恢复显微镜远超过了蔡司的能力 510分辨率和灵敏度。作为一个小组，研究人员描述了 该提案参与微生物和亚微生物的无数方面 生物事件；所有这些都需要反卷积显微镜才能充分 可视化他们特定的生物学问题。在此描述的研究 建议涉及以下荧光可视化：（1）调节蛋白 枯草芽孢杆菌（Hofmeister）中的宠物，spoiiga和pro-i，（2）GFP糖浆 昆虫细胞感染期间的ACMNPV病毒体（Volkman），（3）亚细胞 VIRB蛋白的定位和T转运蛋白的共定位 农杆菌（Zambryski）中的成分，（4）线粒体结构变化 在成纤维细胞（AMES）衰老期间，（5）转录伸长因子SDTLP 在酵母（凯恩）中，（6）剂量补偿和SMC蛋白的3D共定位 在秀丽隐杆线虫上，pachytene染色体（Meyer）和（7） 相间转录因子的表征和共定位 果蝇黑色素果司机细胞（TJIAN）。