Typhoon 9140

描述（由申请人提供）：凝胶和印迹成像是生物医学研究的常规部分。在这项提案中，要求拨款购买台风9410凝胶和印迹成像系统，以取代和升级现有的风暴860成像系统，该系统是由克莱顿大学医学院于2000年提供的校内资金购买的。与Storm 860相比，该设备具有更高的灵敏度，并提供多种检测方式，包括存储荧光粉技术，可对放射性标记的核酸和蛋白质进行成像，以及使用几种波长的直接荧光和化学荧光成像能力，可同时检测多个探针，用于免疫印迹和微阵列分析等应用。这些不同的检测方式将用于支持和加强正在进行的美国国立卫生研究院资助的克莱顿大学的研究，如朊病毒发病机制、皮肤癌发生、抗原受体基因多样化、眼腺发育和调控rna。该仪器还将改善克雷顿研究社区的整体研究基础设施，以开发新的健康相关研究项目。此外，购入9410号台风后，现有的860号台风将转移至文理学院化学系和生物系，以增加本科生的研究和教学机会，从而促进下一代科学家的发展和培训。

DESCRIPTION (provided by applicant): The imaging of gels and blots is a routine part of biomedical research. In this proposal, funds are requested to purchase a Typhoon 9410 gel and blot imaging system to replace and upgrade an existing Storm 860 imaging system acquired using intramural funds provided by the Medical School at Creighton University in 2000. This equipment provides superior sensitivity compared to the Storm 860 and provides several detection modalities, including storage phosphor technology to image radioactively labeled nucleic acids and proteins, and direct fluorescent and chemifluorescent imaging capability using several wavelengths that enables simultaneous detection of multiple probes for applications such as immunoblotting and microarray analysis. These various detection modalities will be used to support and enhance ongoing NIH-funded research at Creighton University in areas such as prion pathogenesis, skin carcinogenesis, antigen receptor gene diversification, ocular gland development, and regulatory RNAs. This instrumentation will also improve the overall research infrastructure available to Creighton's research community to develop new health-related research programs. In addition, the acquisition of the Typhoon 9410 will enable the existing Storm 860 to be transferred to the College of Arts and Sciences Departments of Chemistry and Biology to enhance undergraduate research and teaching opportunities, which will promote the development and training of the next generation of scientists.