UAB High Throughput DNA AutoGenPrep 965

描述(由申请人提供)：转基因小鼠设施(TMF)成立于1991年，是为UAB研究人员高效生产转基因动物模型的中央核心。共享设施主要由大学中心(综合癌症中心(CCC)、综合关节炎、肌肉骨骼和自身免疫中心(CAMAC)、隐性多囊肾病核心中心和代谢骨病中心(CMBD))维护的P30补助金和所提供服务的使用费提供支持。自成立以来，TMF已经为UAB调查人员完成了1000多个DNA或ES细胞显微注射项目，仅在去年一年就有30多名UAB调查人员使用了该设施。在这项提案中，需要资金购买最先进的AutoGenPrep 965(AGP965)全自动高通量机器人仪器，用于转基因小鼠设施中的核提取和纯化。虽然AGP965的设计目的是在几个小时内从多达四个96个深孔板中提纯DNA，用于各种下游应用，但它用于这一应用的主要目的是从小鼠组织样本中提纯高质量的基因组DNA，以促进与多项NIH资助相关的大量小鼠的基因分型。该应用程序代表了该机构内广泛的、由NIH资助的用户基础，其中介绍了18个主要用户的研究成果。AGP965技术的重要性在图表A中得到了说明，其中列出了联合调查人员关于这项提案的出版物，该提案是使用转基因小鼠模型的项目的结果。表3和表4提供了对主要用户和次要用户的联邦支持，估计主要用户群的使用率超过80%。AGP965仪器是一种重要的资源，它将通过多项NIH同行评审的研究拨款显着增强研究项目。

DESCRIPTION (provided by applicant): The Transgenic Mouse Facility (TMF) was established in 1991 as a centralized core for the efficient production of transgenic animal models for UAB investigators. The shared facility is supported primarily by P30 Grants maintained by University Centers (Comprehensive Cancer Center (CCC), Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center (CAMAC), Recessive Polycystic Kidney Disease Core Center, and Center for Metabolic Bone Disease (CMBD)), and by user fees for services provided. Since inception, the TMF has completed more than 1000 DNA or ES cell microinjection projects for UAB investigators with more than 30 UAB investigators using the facility within the last year alone. In this proposal, funds are requested to purchase a state-of- the-art AutoGenPrep 965 (AGP965) fully automated high throughput robotic instrument for nucleic extraction and purification to be housed in the Transgenic Mouse Facility. While the AGP965 is designed to purify DNA from up to four 96 deep well plates in a matter of a few hours for a wide variety of downstream applications, the main intent of its use with this application is the purification of high quality genomic DNA from mouse tissue samples to facilitate genotyping of a large number of mice associated with multiple NIH grants. This application represents a broad, NIH-funded user base within the institution, of which the research endeavors of 18 major users are presented. The importance of the AGP965 technology is illustrated in Exhibit A, which lists the publications from the co-investigators on this proposal that resulted from projects using transgenic mouse models. Federal support of the major and minor users is provided in Tables 3 and 4, with an estimated usage of greater than 80% by the major user base. The AGP965 instrument is an essential resource that will significantly enhance research projects by multiple NIH peer reviewed research grants.