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Gene Targeting Core Facility

基因打靶核心设施

基本信息

批准号：
7669290
负责人：
ROBERT A KESTERSON
金额：
$ 10.2万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7669290
关键词：
Animal Experimentation Animal Model Animals Biological Models Breeding Cancer Center Clinical Nutrition Consultations Core Facility Cryopreservation DNA Disease ES Cell Line Embryo Functional disorder Gene Targeting Genetic Genetically Engineered Mouse Genomics Goals Human Human Resources Image Immune system Individual Injection of therapeutic agent Intervention Laboratories Link Microinjections Mission Modification Molecular Mus Nutritional Study Pathology Phenotype Postdoctoral Fellow Production Productivity Reagent Research Research Personnel Resources Rheumatism Services Stem Cell Development Students System Technology Tissues Training Transgenes Transgenic Animals Transgenic Mice Transgenic Mouse Facility Transgenic Organisms University of Alabama at Birmingham Cancer Center animal facility blastocyst cost cost effective design embryonic stem cell genetic manipulation human disease interest knockout gene lectures mouse genome mouse model recombinase reconstitution vector

项目摘要

The Gene Targeting Core Facility (GTCF) is a fully established core resource to provide expert services for gene targeting of embryonic stem (ES) cells for the development of mouse models. The primary service orovided by this core is that of taking an investigator's gene targeting vector and producing ES cell lines that can be developed into chimeric mice that transmit the pertinent genomic modification(s) through their germline. Investigators utilizing this core have successfully produced a variety of gene knockouts and mouse models of disease. The GTCF is an integral component of the UAB Transgenic Mouse Facility that collectively provides expert services for not only gene targeting via the GTCF, but also blastocyst micro-injection of targeted ES cell clones or DNA constructs for the production of transgenic mouse models, as well as a host of services related to research with mice (e.g. cryopreservation of embryos, rederivation, etc.). The Transgenic Animal Facility is strategically linked with other core resources at UAB including the CCC Small Animal Imaging Core, Clinical Nutrition Research Center Small Animal Phenotyping Core, Research Animal Pathology Laboratory for phenotype assessment, and a specialized mouse-breeding core (Genetically Engineered Mouse and Marker-Assisted Genetics Core). Overall, we have premier resources to make and characterize mouse models for the Center investigators.

基因靶向核心设施（GTCF）是一个完全建立的核心资源，提供专家服务