Next Generation Sequencer
下一代测序仪
基本信息
- 批准号:7791131
- 负责人:
- 金额:$ 50万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-03-11 至 2011-03-10
- 项目状态:已结题
- 来源:
- 关键词:Applications GrantsBiological MarkersClinicClinicalCommitCore FacilityDNADNA-Protein InteractionDetectionDevelopmentEnsureEpigenetic ProcessFunctional RNAFundingGene Expression ProfilingGenerationsGenomeGenomicsHuman ResourcesInstitute of Medicine (U.S.)InstitutionLiftingMaintenanceMarketingMedicineOhioPatientsRegulationResearchResearch Project GrantsResourcesRunningTechnologyTranscriptTranslational ResearchUnited States National Institutes of HealthWorkbasedigitaldisease-causing mutationflexibilitygenome sequencinggenome-widehuman diseaseinstrumentinstrumentationnext generationoperationprograms
项目摘要
DESCRIPTION (provided by applicant): The proposed work in this shared instrumentation grant application comes from many of the clinical and/or translational research programs ongoing at the Cleveland Clinic, a major research institution in the northeast Ohio region. The purpose of this application is to seek funding to purchase a next generation sequencer, the Illumina Genome Analyzer IIx. All but one of the major users is NIH/NCI funded by a variety of mechanisms (R01, P01, U54, etc). The Genome Analyzer has the power and flexibility to enable a wide range of genome-scale applications. The instrument is a direct consequence of the NIH initiative for the $1000 genome and represents the first generation of such technologies to come to the market. The underlying technology can be summarized as massive parallel sequencing (creating 2-4 GB of sequence in a typical 3-5 day run). As detailed in the application, the supported projects primarily include biomarker discovery and clinical/translational sciences studies, representing the unique strengths in our institution and taking advantage of all current applications of the next generation sequencers: 1) digital gene expression profiling: better dynamic range than micro-array based profiling and reliable detection of low-abundance transcripts, 2) ChIP-seq: genome wide profiles of DNA-protein interaction and of epigenetic regulation in translational research programs related to human disease, 3) small or non-coding RNA discovery and 4) genome sequencing: re- sequencing of large genomic regions in patient DNA to uncover disease causing mutations. The acquisition of the Genome Analyzer, with its scale and value, are essential in lifting the currently NIH-funded projects and many other new projects to the next higher level of translational sciences in northeast Ohio. Cleveland Clinic has already committed substantial resources to the development of translational genomics and genomic medicine, including the founding of its Genomic Medicine Institute with a new Genomics Core Facility, personnel to oversee operations and a fiscal plan to ensure its operation and maintenance for the long-term.
描述(由申请人提供):这项共享仪器拨款申请中的拟议工作来自于俄亥俄州东北部地区的一家主要研究机构克利夫兰诊所正在进行的许多临床和/或转化性研究项目。这项申请的目的是寻求资金购买下一代测序仪,Illumina基因组分析仪IIx。除一人外,所有主要用户都是由各种机制(R01、P01、U54等)资助的NIH/NCI。基因组分析仪具有强大的功能和灵活性,可以实现广泛的基因组规模的应用。该仪器是美国国立卫生研究院1000美元基因组计划的直接结果,代表了进入市场的第一代此类技术。基本技术可以概括为大规模并行测序(在典型的3-5天运行中创建2-4 GB的序列)。根据申请的详细情况,所支持的项目主要包括生物标记物发现和临床/翻译科学研究,它们代表了我们机构的独特优势,并利用了下一代测序仪目前的所有应用:1)数字基因表达谱:比基于微阵列的谱图更好的动态范围和对低丰度转录本的可靠检测;2)芯片序列:DNA-蛋白质相互作用的全基因组图谱,以及与人类疾病相关的翻译研究计划中的表观遗传调控;3)小分子或非编码RNA的发现;4)基因组测序:对患者DNA中的大基因组区域进行重新测序,以发现致病突变。收购基因组分析仪及其规模和价值,对于将目前由NIH资助的项目和许多其他新项目提升到俄亥俄州东北部更高水平的翻译科学至关重要。克利夫兰诊所已经为翻译基因组学和基因组医学的发展投入了大量资源,包括建立其基因组医学研究所,建立一个新的基因组核心设施,配备监督运营的人员,以及确保其长期运营和维护的财务计划。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Charis Eng其他文献
Charis Eng的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Charis Eng', 18)}}的其他基金
The 6th Annual International PTEN Symposium: From Patient-Centered Research to Clinical Care
第六届国际 PTEN 研讨会:从以患者为中心的研究到临床护理
- 批准号:
10683454 - 财政年份:2023
- 资助金额:
$ 50万 - 项目类别:
Modeling Autism and Comorbid Cancer Risk in Individuals with Germline PTEN Mutations
种系 PTEN 突变个体的自闭症和共病癌症风险建模
- 批准号:
10704496 - 财政年份:2022
- 资助金额:
$ 50万 - 项目类别:
Modeling Autism and Comorbid Cancer Risk in Individuals with Germline PTEN Mutations
种系 PTEN 突变个体的自闭症和共病癌症风险建模
- 批准号:
10358435 - 财政年份:2022
- 资助金额:
$ 50万 - 项目类别:
Natural history of individuals with autism spectrum disorder and germline PTEN mutations
患有自闭症谱系障碍和种系 PTEN 突变的个体的自然史
- 批准号:
10242080 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
Natural history of individuals with autism spectrum disorder and germline PTEN mutations
患有自闭症谱系障碍和种系 PTEN 突变的个体的自然史
- 批准号:
10701741 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
Deep Sequencing Instrumentation Upgrade - Illumina HiSeq2500
深度测序仪器升级 - Illumina HiSeq2500
- 批准号:
8640603 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
Metagenomic profiling of oral polymicrobial flora in head and neck cancers
头颈癌口腔多微生物菌群的宏基因组分析
- 批准号:
8142045 - 财政年份:2010
- 资助金额:
$ 50万 - 项目类别:
Genetic Alterations that Initiate Follicular Thyroid Carcinogenesis
引发滤泡性甲状腺癌的基因改变
- 批准号:
8505981 - 财政年份:2008
- 资助金额:
$ 50万 - 项目类别:
Genetic Alterations that Initiate Follicular Thyroid Carcinogenesis
引发滤泡性甲状腺癌的基因改变
- 批准号:
8697754 - 财政年份:2008
- 资助金额:
$ 50万 - 项目类别:
Genetic Alterations that Initiate Follicular Thyroid Carcinogenesis
引发滤泡性甲状腺癌的基因改变
- 批准号:
9041528 - 财政年份:2008
- 资助金额:
$ 50万 - 项目类别:
相似海外基金
MRI and Biological Markers of Acute E-Cigarette Exposure in Smokers and Vapers
吸烟者和电子烟使用者急性电子烟暴露的 MRI 和生物标志物
- 批准号:
10490338 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
MRI and Biological Markers of Acute E-Cigarette Exposure in Smokers and Vapers
吸烟者和电子烟使用者急性电子烟暴露的 MRI 和生物标志物
- 批准号:
10353104 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
Investigating pollution dynamics of swimming pool waters by means of chemical and biological markers
利用化学和生物标记物研究游泳池水体的污染动态
- 批准号:
21K04320 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MRI and Biological Markers of Acute E-Cigarette Exposure in Smokers and Vapers
吸烟者和电子烟使用者急性电子烟暴露的 MRI 和生物标志物
- 批准号:
10688286 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
Novel biological markers for immunotherapy and comprehensive genetic analysis in thymic carcinoma
用于胸腺癌免疫治疗和综合遗传分析的新型生物标志物
- 批准号:
20K17755 - 财政年份:2020
- 资助金额:
$ 50万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Examination of Biological Markers Associated with Neurobehavioral and Neuropsychological Outcomes in Military Veterans with a History of Traumatic Brain Injury
与有脑外伤史的退伍军人的神经行为和神经心理结果相关的生物标志物的检查
- 批准号:
10578649 - 财政年份:2019
- 资助金额:
$ 50万 - 项目类别:
Examination of Biological Markers Associated with Neurobehavioral and Neuropsychological Outcomes in Military Veterans with a History of Traumatic Brain Injury
与有脑外伤史的退伍军人的神经行为和神经心理结果相关的生物标志物的检查
- 批准号:
10295141 - 财政年份:2019
- 资助金额:
$ 50万 - 项目类别:
Examination of Biological Markers Associated with Neurobehavioral and Neuropsychological Outcomes in Military Veterans with a History of Traumatic Brain Injury
与有脑外伤史的退伍军人的神经行为和神经心理结果相关的生物标志物的检查
- 批准号:
10041708 - 财政年份:2019
- 资助金额:
$ 50万 - 项目类别:
Examination of Biological Markers Associated with Neurobehavioral and Neuropsychological Outcomes in Military Veterans with a History of Traumatic Brain Injury
与有脑外伤史的退伍军人的神经行为和神经心理结果相关的生物标志物的检查
- 批准号:
9776149 - 财政年份:2019
- 资助金额:
$ 50万 - 项目类别:
Combining biological and non-biological markers to develop a model predictive of treatment response for individuals with depression
结合生物和非生物标志物来开发预测抑郁症患者治疗反应的模型
- 批准号:
2063934 - 财政年份:2018
- 资助金额:
$ 50万 - 项目类别:
Studentship