Deep Sequencing Instrumentation Upgrade - Illumina HiSeq2500

深度测序仪器升级 - Illumina HiSeq2500

• 批准号: 8640603
• 负责人: Charis Eng
• 金额: $ 60万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-15 至 2015-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640603
• 关键词: Biological Markers; Chemistry; Clinic; Clinical; Commit; DNA; DNA Fingerprinting; Development; Ensure; Epigenetic Process; Functional RNA; Funding; Gene Expression Profile; Gene Expression Profiling; Genome; Genomics; Health system; Human; Institute of Medicine (U.S.); Institution; Libraries; Lifting; Maintenance; Marketing; Massive Parallel Sequencing; Medicine; Metagenomics; Ohio; Output; Patients; Reading; Regulation; Research; Research Infrastructure; Research Institute; Research Personnel; Resources; Running; Sampling; Seeds; Services; Small RNA; System; Technology; Time; Transcript; Translational Research; United States National Institutes of Health; base; cost effective; deep sequencing; disease-causing mutation; exome sequencing; flexibility; genetic regulatory protein; genome sequencing; genome-wide; human disease; instrument; instrumentation; metagenome; methylome; next generation; novel; operation; transcriptome sequencing; translational study

DESCRIPTION (provided by applicant): The purpose of this application is to leverage our Illumina Genome Analyzer IIx (GAIIx) next generation sequencer asset to upgrade and better serve the deep sequencing needs of investigators within the Lerner Research Institute and the research enterprise of the Cleveland Clinic Health System by requesting funds to purchase the higher throughput, flexible, state-of-the-art, Illumina HiSeq2500 instrument system. The HiSeq2500 instrument is the latest in the Illumina suite of massively parallel sequencing instruments developed as a direct consequence of the NIH initiative for the $1,000 genome project. It has a higher sequence read output, a rapid-run mode for greater flexibility enabling a wider range of genome-scale applications while being more cost effective to run than the GAIIx. The HiSeq2500 represents the most versatile, in terms of applications capabilities, of the Illumina instruments and technologies to come to the market. The underlying technology can be summarized as massively parallel sequencing using bead-based flowcells with single base synthesis TruSeq v3 chemistry (maximum output 600 Gb of sequence in a typical 10 day high throughput run). As detailed in the application, the instrument and technology will support our projects that primarily include biomarker discovery, transcriptome, methylome, metagenome and clinical/translational studies that represent the unique strengths of our institution. The projects will take advantage of the HiSeq2500 advances of scale, versatility and higher sample multiplexing capability. Higher numbers of output reads per flowcell than the GAIIx leads to higher library multiplexing capability for RNA-seq resulting in less expense per sample, better dynamic range than microarray profiling, the ability to reliably detect low- abundance transcripts and to discover novel transcripts for whole transcriptome, small or non-coding RNA discovery and gene expression profiling. The HiSeq2500 takes less time to complete genome- wide profiles of DNA-protein regulatory interactions, epigenetic regulation, whole genome sequencing, exome sequencing, re-sequencing of large genomic regions in translational research with patient DNA to uncover human disease causing mutations and translational metagenomic studies of human gut flora. The HiSeq2500 is essential to lift the current NIH-funded projects to the next level and to seed new endeavors in northeast Ohio. Cleveland Clinic has already committed substantial resources to the development of translational genomics and genomic medicine by founding the LRI-Genomics Core within the Genomic Medicine Institute and by supporting its operation within the larger Research Core Services infrastructure to ensure its operation and maintenance for the long-term.

描述(申请人提供)：本申请的目的是利用我们的Illumina Genome Analyzer IIx(GAIIx)下一代测序仪资产，通过申请资金购买更高吞吐量、灵活、最先进的Illumina HiSeq2500仪器系统，升级并更好地服务于勒纳研究所和克利夫兰临床医疗系统研究企业的研究人员的深度测序需求。HiSeq2500仪器是Illumina大规模并行测序仪器套件中的最新仪器，该仪器是NIH为1000美元基因组计划而开发的直接结果。它具有更高的序列读取输出，快速运行模式具有更大的灵活性，支持更广泛的基因组规模应用，同时运行起来比GAIIx更具成本效益。就应用功能而言，HiSeq2500代表了即将上市的Illumina仪器和技术中最通用的。基本技术可以概括为使用基于珠子的流动细胞与单碱基合成TruSeq v3化学进行大规模并行测序(在典型的10天高通量运行中最大输出600 GB序列)。如申请中所述，该仪器和技术将支持我们的项目，这些项目主要包括生物标记物发现、转录组、甲基组、元基因组和临床/翻译研究，这些研究代表了我们机构的独特优势。这些项目将利用HiSeq2500在规模、多功能性和更高的样本多路复用能力方面的优势。与GAIIx相比，每个流动细胞的输出读数更高，从而获得更高的RNA-Seq文库多路复用能力，从而使每个样本的成本更低，动态范围比微阵列分析更好，能够可靠地检测低丰度转录本，并为整个转录组、小分子或非编码RNA发现和基因表达分析发现新的转录本。HiSeq2500需要更少的时间来完成DNA-蛋白质调节相互作用、表观遗传调节、全基因组测序、外显子组测序、与患者DNA的翻译研究中大基因组区域的重新测序，以揭示人类疾病导致的突变和人类肠道菌群的翻译元基因组研究。HiSeq2500对于将目前由NIH资助的项目提升到新的水平并在俄亥俄州东北部培育新的努力至关重要。克利夫兰诊所已经为翻译基因组学和基因组医学的发展投入了大量资源，在基因组医学研究所内建立了LRI-基因组学核心，并支持其在更大的研究核心服务基础设施中的运营，以确保其长期运营和维护。