MicroMax-002+ X-ray Generator

描述（由申请人提供）：一个新的x射线设备将使19个NIH资助的赠款的全部潜力得以实现。现有的x射线发生器已经使用了22年，不再工作；现有的区域探测器和低温装置已有10年历史，不再具有成本效益。所要求的设备是：Rigaku MSC， Inc.的MicroMax-002+密封管微聚焦x射线源，加上高性能共焦Max-Flux光学器件，可产生单色聚焦x射线束，其通量比现有RU200旋转阳极发生器大3倍；土星944+ CCD x射线区域探测器，具有94 mm x 94 mm主动图像区域，提供8 MHz读出，15100:1动态范围和高灵敏度；AFC-11计算机控制的四轴测角仪；和X-STREAM-2000低温系统。该设备将为所有生物大分子晶体学实验提供高通量仪器能力，包括筛选晶体质量和膜蛋白和蛋白质- dna复合物的低温保护条件，以及从数百个蛋白质晶体中收集高分辨率数据。一个可靠的内部x射线设备将加速许多晶体的预筛选，优化膜蛋白同步加速器束时间的使用，并使药物发现和蛋白质工程实验的结构确定成为可能。设备项目还要求升级专门设计的单晶显微分光光度计，用于在探索机制的实验中评估蛋白质氧化态和活性位点化学。显微分光光度计是将生物化学性质与晶体结构联系起来的关键，将与新的x射线设备直接结合使用。新设备将使所有七名主要研究人员的项目受益，他们在合作和共享使用现有x射线设备方面有着长期而富有成效的记录。新设备的维护成本低，而且是在美国制造的。新的x射线设施将为研究生和博士后提供晶体学方法的实践培训机会，并将刺激科学互动，提高学习和研究质量，并有助于在学术和生物医学领域保留和创造就业机会。新设备是一项长期投资，因为现有的x射线发生器已经使用了20多年，现有的探测器和低温装置已经使用了10年。

DESCRIPTION (provided by applicant): A new X-ray facility will enable the full potential of 19 NIH sponsored grants to be realized. An existing X-ray generator is 22 years old and no longer functional; the existing area detector and low temperature device is 10 years old and no longer cost-effective to maintain. The equipment requested is: a Rigaku MSC, Inc., MicroMax-002+ sealed tube microfocus X-ray source coupled with a high performance Confocal Max-Flux optic that produces a monochromatic, focused X-ray beam with flux 3X greater than the existing RU200 rotating anode generator; a Saturn 944+ CCD X-ray area detector with 94 mm x 94 mm active image area that provides 8 MHz readout, 15100:1 dynamic range, and high sensitivity; a AFC-11 computer controlled 4-axis goniometer; and a X-STREAM-2000 low temperature system. This equipment will provide high throughput instrumental capacity for all crystallographic experiments with biological macromolecules, including screening for crystal quality and cryoprotection conditions with membrane proteins and protein-DNA complexes, and high resolution data collection from many hundreds of protein crystals. A reliable, in-house X-ray facility will accelerate prescreening of many crystals, optimize the use of synchrotron beam time for membrane proteins, and enable structure determination for drug discovery and protein engineering experiments. Equipment items are also requested to upgrade a specially designed single crystal microspectrophotometer for evaluation of protein oxidation states and active site chemistry in experiments that probe mechanism. The microspectrophotometer is essential to correlate biochemical properties with crystal structures, and will be used in direct conjunction with the new X-ray facility. The new equipment will benefit the projects of all seven principle investigators, who have a long and productive track record of collaboration and shared use of the existing X-ray equipment. The new equipment is cost-effective to maintain, and is manufactured in the United States. The new X-ray facility will provide hands-on training opportunities for graduate students and postdoctoral fellows in crystallographic methods, and will stimulate scientific interactions, enhance the quality of learning and research, and help to retain and create jobs in the academic and biomedical sectors. The new equipment is a long term investment, given that the existing X-ray generator was in use over 20 years, and the existing detector and low temperature device have been in use for 10 years. HEALTH RELEVANCE: Crystallographic experiments require a reliable in-house X-ray facility for high throughput screening of crystal quality and cryoprotection conditions, and for high resolution data collection from many hundreds of protein crystals. The new equipment is required to support these experiments for 19 active NIH grants being directed by seven principle investigators.