Production, Validation and Distribution of the Xenopus ORFeome

描述(由申请人提供)：我们建议生成、验证和分发非洲爪哇ORFeome，我们将其定义为一组完全测序的、经过验证的非洲爪哇cdna克隆，其中包含基因组中编码的每个开放阅读框架(ORF)，其格式是任何ORF序列都可以通过重组工程轻松转移到不同的表达载体阵列中。这一套试剂将极大地减少整个非洲爪哇群落进行的无数功能分析中表征任何蛋白质的时间。但最重要的是，一套ORFeome将允许以目前不可行的方式进行高通量的体内功能基因组筛选，并将利用非洲爪哇系统的特殊优势。这些克隆将不受限制地提供给世界各地的研究人员。非洲爪哇社区完全支持这一项目，在最近的一次PI会议(路易斯湖，2010年9月)上，ORFeome被选为非洲爪哇所需资源的首要任务。 与公共健康相关：我们将开发一种基因组工具，它将极大地提高整个科学界的科学产出，利用非洲爪蛙作为模式生物，被称为ORFeome。这将极大地提高美国国立卫生研究院对非洲爪哇研究的投资回报，该研究目前拥有100多个实验室，相当于NIH每年约1.24亿美元的资金。ORFeome是一种经过高度验证的工具，已成功地提高了人类、蠕虫和酵母实验室的生产率。非洲爪哇社区完全支持这一项目，在最近的一次PI会议(路易斯湖，2010年9月)上，ORFeome被选为非洲爪哇所需资源的首要任务。此外，ORFeome的产生将极大地提高当前基因组信息的有用性，这是社区的第二高优先事项。

DESCRIPTION (provided by applicant): We propose to generate, validate and distribute a Xenopus ORFeome, which we define as a fully sequenced, validated set of Xenopus cDNA clones containing one each of every open reading frame (ORF) encoded in the genome, in a format in which any ORF sequence can be easily transferred using recombineering into a diverse array of expression vectors. This one set of reagents will greatly decrease the time to characterize any protein in the myriad of functional assays carried out by the entire Xenopus community. But most importantly, an ORFeome set will allow high-throughput in vivo functional-genomic screening in manner currently not feasible, and will take advantage of a particular strength of the Xenopus system. These clones will be made available, without restriction, to researchers worldwide. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources. PUBLIC HEALTH RELEVANCE: We will develop a genomic tool that will greatly enhance the scientific output of the entire scientific community that utilizes the frog Xenopus as a model organism, known as the ORFeome. This will greatly enhance the return of the investment of NIH dollars into Xenopus research which currently contains over 100 laboratories and represents ~$124,000,000 of NIH funding per year. An ORFeome is a highly validated tool that has successfully increased the productivity of human, worm and yeast laboratories. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources. Moreover the production of the ORFeome will greatly enhance the usefulness of the current genome information, which is the second highest priority of the community.