Production, Validation and Distribution of the Xenopus ORFeome

描述(由申请人提供)：我们建议生成、验证和分发非洲爪哇ORFeome，我们将其定义为一组完全测序的、经过验证的非洲爪哇cdna克隆，其中包含基因组中编码的每个开放阅读框架(ORF)，其格式是任何ORF序列都可以通过重组工程轻松转移到不同的表达载体阵列中。这一套试剂将极大地减少整个非洲爪哇群落进行的无数功能分析中表征任何蛋白质的时间。但最重要的是，一套ORFeome将允许以目前不可行的方式进行高通量的体内功能基因组筛选，并将利用非洲爪哇系统的特殊优势。这些克隆将不受限制地提供给世界各地的研究人员。非洲爪哇社区完全支持这一项目，在最近的一次PI会议(路易斯湖，2010年9月)上，ORFeome被选为非洲爪哇所需资源的首要任务。

DESCRIPTION (provided by applicant): We propose to generate, validate and distribute a Xenopus ORFeome, which we define as a fully sequenced, validated set of Xenopus cDNA clones containing one each of every open reading frame (ORF) encoded in the genome, in a format in which any ORF sequence can be easily transferred using recombineering into a diverse array of expression vectors. This one set of reagents will greatly decrease the time to characterize any protein in the myriad of functional assays carried out by the entire Xenopus community. But most importantly, an ORFeome set will allow high-throughput in vivo functional-genomic screening in manner currently not feasible, and will take advantage of a particular strength of the Xenopus system. These clones will be made available, without restriction, to researchers worldwide. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources.