Production, Validation and Distribution of the Xenopus ORFeome

描述（由申请人提供）：我们提出产生、验证和分发非洲爪蟾ORFeome，我们将其定义为完全测序的、验证的非洲爪蟾cDNA克隆集，其包含基因组中编码的每个开放阅读框（ORF）中的每一个，以一种形式，其中任何ORF序列可以使用重组工程容易地转移到不同的表达载体阵列中。这一套试剂将大大减少整个非洲爪蟾群落进行的无数功能测定中表征任何蛋白质的时间。但最重要的是，ORFeome组将允许以目前不可行的方式进行高通量体内功能基因组筛选，并将利用非洲爪蟾系统的特定优势。这些克隆体将不受限制地提供给全世界的研究人员。非洲爪蟾社区完全支持这个项目，在最近的PI会议上（露易丝湖，2010年9月），ORFeome被选为非洲爪蟾资源的最高优先级。

DESCRIPTION (provided by applicant): We propose to generate, validate and distribute a Xenopus ORFeome, which we define as a fully sequenced, validated set of Xenopus cDNA clones containing one each of every open reading frame (ORF) encoded in the genome, in a format in which any ORF sequence can be easily transferred using recombineering into a diverse array of expression vectors. This one set of reagents will greatly decrease the time to characterize any protein in the myriad of functional assays carried out by the entire Xenopus community. But most importantly, an ORFeome set will allow high-throughput in vivo functional-genomic screening in manner currently not feasible, and will take advantage of a particular strength of the Xenopus system. These clones will be made available, without restriction, to researchers worldwide. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources.