Pressure-jump apparatus for time-resolved and static X-ray diffraction studies of conformational/phase transitions & structure in soft matter

用于构象/相变的时间分辨和静态 X 射线衍射研究的压力跳跃装置

• 批准号: ST/F001509/1
• 负责人: Nicholas Terrill
• 金额: $ 3.78万
• 依托单位: Diamond Light Source
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=ST%2FF001509%2F1
• 关键词: Pressure; jump; apparatus; time; resolved

There is currently a pressing need for the development of techniques that will allow experimentalists to probe the time-dependent structural behaviour of out-of-equilibrium soft condensed matter systems. An ideal technique for monitoring and triggering the structural evolution of such transformations within the millisecond timescale is time-resolved X-ray scattering, employing pressure as the triggering variable. The p-jump technique can be applied quite generally to the study of soft condensed matter and biomolecular phase transformations, such as lyotropic liquid crystal phase transitions, micelle-vesicle transformations, DNA unwinding, and protein folding. To this end we aim to develop and construct a millisecond pressure-jump X-ray cell for use at the Non-Crystalline Scattering beamline I22 at Diamond. We will also develop user-friendly apparatus control, integrated with the beamline software currently under development for I22. Although this apparatus is primarily designed for pressure-jump experiments, it can also be used for rapid scanning of pressure in the range 1 bar - 5 kbar, allowing pressure-temperature phase diagrams to be determined rather efficiently. It should also be noted that the cell will be capable of holding a fixed pressure for specific experiments.

目前，迫切需要发展的技术，将允许实验学家探测的时间依赖的结构行为的非平衡软凝聚态系统。监测和触发毫秒时间尺度内这种转变的结构演变的理想技术是时间分辨X射线散射，采用压力作为触发变量。p-jump技术可广泛应用于软凝聚态和生物分子相变的研究，如溶致液晶相变、胶束-囊泡相变、DNA解旋和蛋白质折叠。为此，我们的目标是开发和构建一个毫秒级的压力跳变的X射线细胞在非晶体散射光束线I22钻石使用。我们还将开发用户友好的仪器控制，与目前正在开发的I22光束线软件集成。虽然该装置主要设计用于压力跃变实验，但它也可用于在1 bar - 5 kbar范围内快速扫描压力，从而能够相当有效地确定压力-温度相图。还应注意的是，所述池将能够保持用于特定实验的固定压力。

项目成果

Columnar Phases in Liquid Crystal Dendrimers: Variable Pressure X-Ray Diffraction

液晶树枝状聚合物中的柱状相：变压 X 射线衍射

• DOI: 10.1080/15421406.2011.570217
• 发表时间: 2011
• 期刊: Molecular Crystals and Liquid Crystals
• 影响因子: 0.7
• 作者: Richardson R

Pressure-temperature phase behaviour of natural sphingomyelin extracts

天然鞘磷脂提取物的压力-温度相行为

• DOI: 10.1039/c1sm06703f
• 发表时间: 2012
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: Shaw K

Hydrostatic pressure effects on the lamellar to gyroid cubic phase transition of monolinolein at limited hydration.

• DOI: 10.1021/la3025843
• 发表时间: 2012-08
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: T. Tang;N. Brooks;C. Jeworrek;O. Ces;N. Terrill;R. Winter;R. Templer;J. Seddon