Administrative Core

行政核心

基本信息

  • 批准号:
    8305004
  • 负责人:
  • 金额:
    $ 28.83万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
  • 资助国家:
    美国
  • 起止时间:
    至 2013-07-31
  • 项目状态:
    已结题

项目摘要

FACILITIES AVAILABLE Our five laboratories are adequate to carry out the specific five projects as requested. There are in addition a number of collective facilities, which we share. The labs have a media kitchen with approximately 600 sq. ft. of space dedicated to preparation of Drosophila media and worm plates. Artificial seawater is also produced by this facility. The kitchen currently contains its own autoclave, office, and storage facilities. In addition, the labs share a special space in the basement of the Bassine building ("the pit": approximately 1000 sq. ft.) in which several fly incubators are housed that are used to monitor circadian and thermal activity. The light tight and constant temperature nature of this space makes it optimal for entrainment in constant dark as well as controlled light-dark and temperature conditions. This is where much of the behavioral monitoring takes place. In addition to this there is a 60 sq ft. temperature and humidity controlled Harris room in the Garrity/Griffith space that is used for cold behavior and learning. All of the labs have access to available departmental resources including a phosphorimager facility, confocal facility, microarray/FACS facility, etc. The Confocal laboratory is directed by and used heavily by all laboratories. In addition to this there is also a shared Imaging Facility that has a two-photon microscope and two real-time epifluorescence microscopes. The Fly labs share an injection facility (320 sq. ft.) with two microinjection setups for the generation of transgenic flies. The Microarray/FACS Facility are shared resources 2 buildings away. These facilities contain Affymetrix microarray processing instrumentation (hybridization oven, fluidics station, and scanner), an Agilent 2100 Bioanalyzer system, and a BD FACSAria Flow Cytometer. We have a NMR facility, and a new MALDI and peptide analysis center. The structural biology group at Brandeis owns and operates a superb state-of-the art electron microscope facility. A machine shop and electronics shop are available to the Biology Department and the Science library is located in the adjacent building. ADMINISTRATIVE ARRANGEMENTS The PI of this grant is and has been M. Rosbash for the past twenty-three years. Hall and White who played important leadership roles during the past five years of the grant are passing the torch to Marder and Griffith. Rosbash is principally in charge of grant management and both Rosbash and Marder are responsible for overall scientific leadership and quality control. Griffith is in charge of the media kitchen and other aspects of the Core, including oversight of the microscope facility and its technician. Rosbash's assistant/lab manager Heather Felton, who holds a doctorate in Molecular Biology, as well as the Biology Department administrator Barbara Wrightson provide administrative support for grant-related matters. Felton is an HHMI employee and Wrightson is 100% paid by Brandeis University. All five Pis are members of the Brandeis Biology Department, which is their primary affiliation from an administrative standpoint, and of the Volen Center for Complex Systems. Rosbash has received 100% salary from HHMI since 1989 eliminating any salary from this grant at that time. Most students and post-docs in the group attend the ample seminars and journal clubs hosted by the Biology Department, the Biochemistry Department and the Neuroscience community. There are also Program Project monthly seminars to foster closer contact between the five laboratories. The fly groups hold a monthly "FlyFest" at which both inside and outside speakers present their work.
可用设施 我们的五个实验室足以执行所要求的五个具体项目。此外,还有一个 我们共享的一些集体设施。实验室有一个大约600平方米的媒体厨房。英尺的 空间专门用于准备果蝇培养基和蠕虫平板。人工海水也是由 这个设施。厨房目前包含自己的高压灭菌器,办公室和存储设施。此外,实验室 在Bassine大楼的地下室共享一个特殊的空间(“坑”:约1000平方米)。英尺)。其中 安置了几个苍蝇培养箱,用于监测昼夜节律和热活动。光线紧密, 该空间的恒温性质使其最适合在恒定黑暗中夹带, 控制光暗和温度条件。这是大部分行为监测发生的地方。 除此之外,还有一个60平方英尺。温度和湿度控制哈里斯室在加里蒂/格里菲斯 用于冷行为和学习的空间。 所有的实验室都可以使用现有的部门资源,包括磷光成像设备,共聚焦 共聚焦实验室是由所有人指导和大量使用的 laboratories.除此之外,还有一个共享的成像设备,该设备具有双光子显微镜, 两个实时荧光显微镜。Fly实验室共用一个注射设施(320平方米)。英尺)。具有两 用于产生转基因果蝇的显微注射装置。微阵列/流式细胞仪是共享资源 两栋楼外。这些设施包括Affymbre微阵列处理仪器(杂交 烘箱、射流站和扫描仪)、Agilent 2100生物分析仪系统和BD FACSAria流式细胞仪。 我们有一个核磁共振设施,和一个新的MALDI和肽分析中心。结构生物学小组在 布兰迪斯拥有并经营着一个一流的国家最先进的电子显微镜设施。一个机械车间, 电子商店是提供给生物系和科学图书馆位于毗邻 建设 行政安排 本补助金的PI是并一直是M。Rosbash在过去的23年里。霍尔和白色谁发挥 重要的领导作用,在过去五年的赠款是传递火炬给马德和格里菲斯。 Rosbash主要负责赠款管理,Rosbash和Marder负责 全面的科学领导和质量控制。格里菲斯负责媒体厨房和其他方面, 核心,包括监督显微镜设施及其技术人员。Rosbash的助理/实验室经理 拥有分子生物学博士学位的石楠费尔顿,以及生物系主任 Barbara Wrightson为赠款相关事宜提供行政支持。费尔顿是HHMI的员工, Wrightson由布兰迪斯大学支付100%的费用。五个私家侦探都是布兰迪斯生物系的成员, 这是他们的主要附属机构,从行政的角度来看,和沃伦中心的复杂 系统.自1989年以来,Rosbash从HHMI获得了100%的工资, 当时大多数学生和博士后在集团参加丰富的研讨会和杂志俱乐部主办的 生物系、生物化学系和神经科学界。也有 计划项目每月举办研讨会,以促进五个实验室之间的密切联系。苍蝇团体举行了一个 每月一次的“FlyFest”,在此期间,内部和外部的演讲者都将展示他们的作品。

项目成果

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MICHAEL ROSBASH其他文献

MICHAEL ROSBASH的其他文献

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{{ truncateString('MICHAEL ROSBASH', 18)}}的其他基金

A new tool for the cell-specific identification of RNA binding protein targets
用于细胞特异性鉴定 RNA 结合蛋白靶标的新工具
  • 批准号:
    8640299
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
2013 Chronobiology GRC/GRS
2013年时间生物学GRC/GRS
  • 批准号:
    8529855
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
A new tool for the cell-specific identification of RNA binding protein targets
用于细胞特异性鉴定 RNA 结合蛋白靶标的新工具
  • 批准号:
    8735924
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
Addressing Protein Synthesis Regulation within Small Numbers of Discrete Neurons
解决少量离散神经元内的蛋白质合成调控问题
  • 批准号:
    10586226
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
A new tool for the cell-specific identification of RNA binding protein targets
用于细胞特异性鉴定 RNA 结合蛋白靶标的新工具
  • 批准号:
    9112980
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
Addressing protein synthesis regulation within small numbers of discrete neurons
解决少量离散神经元内的蛋白质合成调节问题
  • 批准号:
    10091418
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
A new tool for the cell-specific identification of RNA binding protein targets
用于细胞特异性鉴定 RNA 结合蛋白靶标的新工具
  • 批准号:
    8913110
  • 财政年份:
    2013
  • 资助金额:
    $ 28.83万
  • 项目类别:
2011 Chronobiology Gordon Research Conference
2011年时间生物学戈登研究会议
  • 批准号:
    8118328
  • 财政年份:
    2011
  • 资助金额:
    $ 28.83万
  • 项目类别:
Core Facilities for Neurobiology at Brandeis
布兰迪斯神经生物学核心设施
  • 批准号:
    8387998
  • 财政年份:
    2003
  • 资助金额:
    $ 28.83万
  • 项目类别:
Computational Core
计算核心
  • 批准号:
    8374478
  • 财政年份:
    2003
  • 资助金额:
    $ 28.83万
  • 项目类别:

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