Regulation of Cell-extracellular Matrix Interactions at the Brain Surface

脑表面细胞-细胞外基质相互作用的调节

• 批准号: 8109914
• 负责人: HUAIYU HU
• 金额: $ 27.87万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-20 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8109914
• 关键词: Area; Astrocytes; Basement membrane; Binding; Brain; Cells; Cerebral cortex; Coculture Techniques; Development; Dolichyl-phosphate-mannose-protein mannosyltransferase; Dystroglycan; ECM receptor; Enzymes; Extracellular Matrix; Fibroblasts; Gene Delivery; Genes; Glycoproteins; Goals; Health; Hereditary Disease; Integrins; Knock-out; Knockout Mice; Knowledge; Label; Lead; Light; Link; Maintenance; Mannose; Mediating; Meningeal; Meninges; Molecular; Movement; Muscle eye brain disease; Muscular Dystrophies; Mutation; N-Acetylglucosaminyltransferases; Neuroglia; Neurons; Pathogenesis; Physiologic pulse; Play; Polysaccharides; Proteins; Radial; Regulation; Research; Role; Surface; System; Walker-Warburg syndrome; brain malformation; cell motility; congenital muscular dystrophy; gene therapy; glycosylation; glycosyltransferase; improved; insight; lissencephaly; meetings; overexpression; prevent; protein function; receptor; restoration

DESCRIPTION (provided by applicant): Congenital muscular dystrophies (CMDs) with brain malformations are genetic diseases. Brain malformation involves movement of neurons out of the cerebral cortex through breaches of the pial basement membrane (PBM). We propose to study the critical molecules underlying formation of the PBM by radial glia. O-mannosyl glycosylation appears to have an important role. Also further studies of POMT2 conditional knockout mice may shed light on disruptions of the PBM that mediate migration of cells out of the brain. Our hypothesis is that radial glia have a key role in assembling the PBM. Specific Aims are to investigate: 1. The role of radial glia in assembly of the pial basement membrane (PBM). 2. The mechanisms of PBM abnormalities in POMT2 knockout mice. 3. The feasibility of using Large in gene therapy. The proposed research will provide new and important insights into how protein O- mannosyl glycosylation regulates the formation and maintenance of the PBM. It should also yield insights on mechanisms underlying brain malformations in type II lissencephaly. Better knowledge of the key molecules involved in PBM disruptions should lead to potential gene therapies. Gene delivery to restore protein functions should be directed at those cells that organize the formation of the PBM. The proposed research should lead to an improved understanding of the pathogenesis of muscular dystrophies in general and their treatment. PUBLIC HEALTH RELEVANCE: Type II lissencephaly in congenital muscular dystrophies is caused by disruptions of the pial basement membrane. Aberrant cell-extracellular matrix interaction at the brain surface is the cause of those disruptions. Studies will determine the cells and their molecules responsible for organizing the formation of the pial basement membrane to identify potential targets for gene therapy.

描述（由申请人提供）：先天性肌营养不良症（CMD）伴脑畸形是遗传性疾病。脑畸形涉及神经元通过软脑膜基底膜（PBM）的破裂而移出大脑皮层。我们建议研究放射状胶质细胞形成PBM的关键分子。O-甘露糖基化似乎具有重要作用。POMT 2条件性敲除小鼠的进一步研究也可能揭示介导细胞迁移出脑的PBM的破坏。我们的假设是放射状胶质细胞在组装PBM中起关键作用。具体目的是调查：1。放射状胶质细胞在软脑膜基底膜组装中的作用。2. POMT 2基因敲除小鼠PBM异常的机制3.将Large用于基因治疗的可行性。这项研究将为蛋白质O-甘露糖基化如何调节PBM的形成和维持提供新的重要见解。它也应该产生的机制，在II型无脑回畸形的脑畸形的见解。更好地了解参与PBM破坏的关键分子应该会导致潜在的基因治疗。恢复蛋白质功能的基因递送应该针对组织PBM形成的那些细胞。拟议的研究应导致更好地了解一般肌营养不良症的发病机制及其治疗。公共卫生相关性：先天性肌营养不良症的II型无脑回畸形是由软脑膜基底膜破坏引起的。脑表面异常的细胞-细胞外基质相互作用是这些破坏的原因。研究将确定负责组织软脑膜基底膜形成的细胞及其分子，以确定基因治疗的潜在靶点。