COMPARTMENTAL ANALYSIS OF PROTEOMIC BIOMARKERS DURING INTRA-UTERINE INFECTIONS

子宫内感染期间蛋白质组生物标志物的区室分析

• 批准号: 8173276
• 负责人: Peta Louise Grigsby
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173276
• 关键词: ANXA2 gene; Biological Markers; Characteristics; Clinical Management; Computer Retrieval of Information on Scientific Projects Database; Diagnostic; Early Diagnosis; Endocrine; Experimental Models; Funding; Grant; Homeostasis; Infection; Institution; Insulin-Like Growth-Factor Binding Protein 1; Intervention; Modeling; Molecular Profiling; Physiological Adaptation; Placental Circulation; Premature Birth; Proteomics; Research; Research Personnel; Resources; Risk; Source; Staging; Therapeutic; United States National Institutes of Health; Ureaplasma urealyticum biovar 1; Woman; Work; career development; expectation; fetal; hemodynamics; improved; intraamniotic infection; nonhuman primate; respiratory

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of this research and career development grant are to utilize a nonhuman primate experimental model of intra-uterine infection with Ureaplasma parvum to characterize the temporal and mechanistic interactions among biomarker expression profiles (i.e., IGFBP-1 proteolytic fragments, calgranulins A and B and annexin II), in maternal and fetal compartments at defined stages of ascending uterine infection (from choriodecidual to intra-amniotic models). It is our hypothesis that specific biomarker profiles (spatial and temporal characteristics), will serve as surrogates for the stage of progression of ascending intra-uterine infection. An important aspect of this study is to determine the fetal physiologic adaptations to intra-amniotic infection (i.e., endocrine status, respiratory parameters and hemodynamic changes in regional and placental circulations), and to assess the efficacy of a combined therapeutic approach for improving fetal cardio-respiratory homeostasis. The work proposed is unique in its combined use of diagnostic and interventional strategies in a nonhuman primate model of intra-uterine infection (experimental choriodecidual and intra-amniotic stages). It is our expectation that the results of these studies will advance clinical management and facilitate the early diagnosis of women that are at risk for preterm delivery as a consequence of intra-uterine infection.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这项研究和职业发展资助的目的是利用微小解脲支原体宫内感染的非​​人灵长类动物实验模型来表征在子宫上行感染特定阶段的母体和胎儿区室中生物标志物表达谱（即 IGFBP-1 蛋白水解片段、钙颗粒蛋白 A 和 B 以及膜联蛋白 II）之间的时间和机制相互作用。 （从绒毛膜蜕膜模型到羊膜内模型）。我们的假设是，特定的生物标志物谱（空间和时间特征）将作为宫内上行感染进展阶段的替代指标。这项研究的一个重要方面是确定胎儿对羊膜内感染的生理适应（即内分泌状态、呼吸参数以及局部和胎盘循环的血流动力学变化），并评估联合治疗方法改善胎儿心肺稳态的功效。这项工作的独特之处在于在非人灵长类动物子宫内感染模型（实验性绒毛膜蜕膜阶段和羊膜内阶段）中结合使用了诊断和干预策略。我们期望这些研究的结果将促进临床管理并促进对因宫内感染而面临早产风险的妇女的早期诊断。