COMPARTMENTAL ANALYSIS OF PROTEOMIC BIOMARKERS DURING INTRA-UTERINE INFECTIONS

子宫内感染期间蛋白质组生物标志物的区室分析

• 批准号: 8357791
• 负责人: Peta Louise Grigsby
• 金额: $ 5.82万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357791
• 关键词: Accounting; Amniotic Fluid; Animal Model; Bacteria; Biological Markers; Cervical Ripening; Characteristics; Clinical; Decidua; Endometrial; Event; Fetal Membranes; Funding; Genital system; Grant; Indolent; Infection; Inflammation; Inflammation Mediators; Inflammatory Response; Modeling; Mycoplasma; National Center for Research Resources; Natural History; Pathogenicity; Pregnancy; Premature Birth; Premature Labor; Premature Rupture Fetal Membranes; Primates; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Source; Staging; Streptococcus Group B; Syndrome; Time; United States National Institutes of Health; Ureaplasma; Ureaplasma urealyticum biovar 1; Uterine Contraction; Virulence; Woman; amniotic cavity; cost; intraamniotic infection; microbial; nonhuman primate; preimplantation; premature; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our specific research aims focus upon defining the temporal and compartmental relationships among specific biomarkers and the biologic and clinical manifestations of early and late stages of ascending uterine infection (from choriodecidual to intra-amniotic models). Accumulating evidence indicates that intra-amniotic infection by genital mycoplasmas is a predominant cause of early preterm birth. The spatial and temporal characteristics of progressive stages of ascending infection and their relationship to biomarkers and antecedent signs of preterm labor have not been clearly defined in women. Similarly, previous animal models of intra-uterine infection have not taken into account that inflammatory responses in the decidua, fetal membranes and amniotic cavity may represent independent events that initiate different aspects of the preterm labor syndrome (e.g., premature cervical ripening, premature rupture of membranes, or uterine contractions). We demonstrated that choriodecidual inflammation with group B streptococcus is a transitional stage of ascending intra-uterine infection characterized by a graded response in amniotic fluid pro-inflammatory mediators, depending upon the presence or absence of bacteria in the amniotic fluid. Given the evidence for pre-implantation endometrial colonization with genital mycoplasmas, and their indolent natural history during pregnancy, ascending infection with ureaplasmas is likely to be quite different (i.e., time course or microbial characteristics, such as pathogenicity/virulence). The current proposal is expanding our understanding of the pathophyisology of choriodecidual inflammation/infection with Ureaplasma parvum in a non-human primate model and evaluating the spatial and temporal characteristics of progressive stages of ascending infection and their relationship to biomarkers and antecedent signs of preterm labor.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们的具体研究旨在定义特定生物标志物之间的时间和隔室关系，以及升升子宫感染的早期和晚期的生物学和临床表现（从绒毛膜肌中到肿瘤内模型）。积累的证据表明，生殖器支原体上的肿瘤内感染是早产早期出生的主要原因。妇女尚未明确定义上升感染及其与生物标志物的关系及其与生物标志物的关系及其与生物标志物的关系及其与生物标志物的关系的空间和时间特征。同样，先前的河内感染动物模型尚未考虑到Decidua，胎儿膜和羊膜腔中的炎症反应可能代表着独立的事件，这些事件启动了早产综合征的不同方面（例如，过早的宫颈成熟，，膜的早产，膜的早产，膜或uterine缩合）。我们证明，与B组链球菌的脉络膜炎症是升高炎症液中炎症介质中渐变性反应的过渡阶段，取决于羊水液中细菌的存在或不存在。鉴于有证据表明，植入前的子宫内膜定殖与生殖器支原体及其在怀孕期间的自然病史，尿素倍率的升高可能会大不相同（即，时间过程或微生物特征，例如致病性/毒力）。当前的提议正在扩大我们对非人类灵长类动物模型中绒毛膜炎症/感染的病理学病理学的理解，并评估了升级感染的渐进阶段的空间和时间特征及其与生物标志物的关系及其与生物标志物的关系以及早产劳动的迹象。