IN VIVO SAFETY AND EFFICACY OF CAP FILM AND MICROBICIDE COMBINATIONS

帽膜和杀菌剂组合的体内安全性和功效

• 批准号: 8172991
• 负责人: CECILIA C CHENG-MAYER
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172991
• 关键词: Adult; Animals; Attenuated; Computer Retrieval of Information on Scientific Projects Database; Dose; Female; Film; Funding; Gel; Genes; Grant; HIV-1; Hour; Institution; Macaca; Methods; RNA-Directed DNA Polymerase; Research; Research Personnel; Resources; Safety; Source; United States National Institutes of Health; Vagina; Viral; Virus; in vivo; microbicide; research study; simian human immunodeficiency virus; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: We proposed to establish the efficacy MIV-160 alone against simian human immunodeficiency virus containing the reverse transcriptase gene of HIV-1 (SHIV-RT). Six animals were used for this experiment. Methods: Three (3) ml of MIV-160, 500¿M/ml in hydroxymethycellulose (HEC) gel was applied vaginal into 6 adult Indian female macaques. Half -hour post gel application, each animal was challenged vaginally with 1 ml high dose 10000 TCID50 of SHIV-RT. Again, 2 ml each of MIV-160 was applied vaginally in the respective animals. 30 minutes post SHIV-RT challenge. Results/Discussion: All animals were infected. MIV-160 failed to impede transmission of the impute virus. All animals had high viral set points which indicate that MIV-160 did not alter nor attenuate SHIV-RT.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 背景：我们提议确定 MIV-160 单独对抗含有 HIV-1 逆转录酶基因 (SHIV-RT) 的猿猴人类免疫缺陷病毒的功效。该实验使用了六只动物。 方法：将三 (3) ml 羟甲基纤维素 (HEC) 凝胶中的 MIV-160（浓度为 500 µM/ml）注入 6 只成年印度雌性猕猴的阴道内。凝胶应用后半小时，每只动物用1ml高剂量10000TCID50的SHIV-RT进行阴道攻击。 再次将各2ml的MIV-160通过阴道施用到相应的动物中。 SHIV-RT 挑战后 30 分钟。 结果/讨论：所有动物均被感染。 MIV-160 未能阻止推定病毒的传播。 所有动物均具有高病毒设定点，这表明 MIV-160 不会改变或减弱 SHIV-RT。