IN VIVO SAFETY AND EFFICACY OF CAP FILM AND MICROBICIDE COMBINATIONS

帽膜和杀菌剂组合的体内安全性和功效

• 批准号: 8172991
• 负责人: CECILIA C CHENG-MAYER
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172991
• 关键词: Adult; Animals; Attenuated; Computer Retrieval of Information on Scientific Projects Database; Dose; Female; Film; Funding; Gel; Genes; Grant; HIV-1; Hour; Institution; Macaca; Methods; RNA-Directed DNA Polymerase; Research; Research Personnel; Resources; Safety; Source; United States National Institutes of Health; Vagina; Viral; Virus; in vivo; microbicide; research study; simian human immunodeficiency virus; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: We proposed to establish the efficacy MIV-160 alone against simian human immunodeficiency virus containing the reverse transcriptase gene of HIV-1 (SHIV-RT). Six animals were used for this experiment. Methods: Three (3) ml of MIV-160, 500¿M/ml in hydroxymethycellulose (HEC) gel was applied vaginal into 6 adult Indian female macaques. Half -hour post gel application, each animal was challenged vaginally with 1 ml high dose 10000 TCID50 of SHIV-RT. Again, 2 ml each of MIV-160 was applied vaginally in the respective animals. 30 minutes post SHIV-RT challenge. Results/Discussion: All animals were infected. MIV-160 failed to impede transmission of the impute virus. All animals had high viral set points which indicate that MIV-160 did not alter nor attenuate SHIV-RT.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 背景：我们提议仅建立有效性MIV-160，以针对含有HIV-1（SHIV-RT）逆转录酶基因的邻近人类免疫缺陷病毒（SHIV-RT）。该实验使用了六只动物。方法：将三（3）毫升的MIV-160，500°M/mL羟基纤维素（HEC）凝胶中的500毫升涂在6个成年印度女性猕猴中。半小时凝胶施用后，每只动物都被挑战阴道，1 ml高剂量10000 TCID50 SHIV -RT。同样，将MIV-160的每种2毫升阴道涂在各自的动物中。 SHIV-RT挑战后30分钟。 结果/讨论：所有动物都被感染。 MIV-160未能阻碍插发病毒的传播。所有动物的病毒设定点都高，表明MIV-160没有改变或衰减SHIV-RT。