PHENOTYPIC AND GENOTYPIC DETERMINANTS OF SHIV PATHOGENESIS

SHIV 发病的表型和基因型决定因素

• 批准号: 8172945
• 负责人: CECILIA C CHENG-MAYER
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172945
• 关键词: Animals; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Control Animal; Disease; Funding; Grant; Institution; Pathogenesis; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Viral; Virus; in vivo; memory CD4 T lymphocyte

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We investigated pathogenesis and transmissibility of SHIVSF162P3N (P3N) and the clone, whether the clone could fully recapitulate the in vivo replicative characteristics of the parental isolate. Both SHIVSF162P3N, (P3N) and the clone were mucosally transmissible, preferentially depleted memory CD4 T cells. Fifteen (15) animals were rectally challenged, 3 animals with P3N plus clone and 12 animals with the clone alone. Both virus infected rectally, 6/12 animals controlled the virus in the clone group, whereas one animals challenged with the mixture virus ( P3N and clone) was able to control the virus. Preliminary results indicate that both virus were transmitted mucosally with no significant difference in viral set points and progression to disease.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们研究了SHIVSF 162 P3 N（P3 N）和克隆的致病性和遗传性，克隆是否能完全重现亲本分离株的体内复制特征。SHIVSF 162 P3 N（P3 N）和克隆都是粘膜传播的、优先耗尽的记忆性CD 4 T细胞。 对15只动物进行直肠攻毒，3只动物使用P3 N加克隆，12只动物仅使用克隆。 两种病毒均经直肠感染，克隆组中6/12的动物控制了病毒，而用混合病毒（P3 N和克隆）攻击的一只动物能够控制病毒。 初步结果表明，两种病毒均经粘膜传播，在病毒设定点和疾病进展方面无显著差异。