CORE--Gene Expression Analysis

Core C -基因表达分析的目的是为项目提供高度的 需要专门的方法：（1）从人类和动物样本中提取高质量的RNA 并进行质量控制测试;（2）处理RNA以产生高质量的标记cRNA， 杂交到微阵列;（3）提供图像捕获和数据挖掘软件， 分析由微阵列技术产生的大量基因表达数据;（4） 为每个项目量身定制数据分析;以及（5）通过以下方式量化mRNA水平： TaqMan真实的时间PCR用于微阵列筛选所选择的基因。基因表达 分析核心在使用不同的Affymbre微阵列数据分析方面具有广泛的专业知识 用于数据挖掘的方法和计算工具，特别是从肺部获得的样本的数据挖掘 通过支气管镜检查/刷拭和支气管肺泡灌洗对人类志愿者进行了研究。核心在其 处理Affymbus基因芯片设备，以及最先进的软件包， 可以通过威尔康奈尔计算生物医学研究所获得。TaqMan实时 RT-PCR用于RNA定量可通过该核心，使用高通量96孔应用 生物系统7500和软件为感兴趣的基因设计特异性引物，以及 技术支持和培训，以协助调查人员在每个项目中开展工作 TaqMan分析。核心将为所有五个项目提供同等的资源。

The purpose of Core C - Gene Expression Analysis is to provide the projects with the highly specialized methodology required to: (1) extract high quality RNA from human and animal samples and perform quality control tests; (2) process RNA to produce high quality labeled cRNA for hybridization to the microarrays; (3) provide image capture and data mining software necessary for analyzing the vast amount of gene expression data generated by microarray technology; (4) perform data analysis tailored for each individual project; and (5) to quantify mRNA levels by TaqMan real time PCR for the genes selected by the microarray screen. The Gene Expression Analysis Core has extensive expertise in Affymetrix microarray data analysis using different methods and computational tools for data mining, particularly of samples obtained from the lungs of human volunteers by bronchoscopy/brushing, and bronchoalveolar lavage. The Core has at its disposal the Affymetrix GeneChip equipment, as well as state-of-the-art software packages that are available through the Weill Cornell Institute for Computational Biomedicine. TaqMan real-time RT-PCR for RNA quantitation is available through this Core, using high throughput 96 well Applied Biosystems 7500 and software to design specific primers for the genes of interest, as well as technical support and training to assist the investigators in each of the projects to carry out TaqMan analysis. The Core will devote equal resources to all five projects.