A new serum test for the differential diagnosis of pituitary masses

鉴别诊断垂体肿块的新血清检测

• 批准号: 8017624
• 负责人: PATRIZIO CATUREGLI
• 金额: $ 8万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8017624
• 关键词: Anterior Pituitary Hormones; Antibodies; Appearance; Autoantigens; Autoimmune Process; Autoimmunity; Biological Assay; Cell Line; Clinical; Collection; Development; Diagnosis; Differential Diagnosis; Disease; Enzyme-Linked Immunosorbent Assay; Experimental Models; Expression Library; Gel; Genetic Transcription; Grant; Histologic; Histopathology; Hormones; Human; Hypopituitarism; Immune; Immune system; Immunization; Immunoblotting; In Vitro; Knowledge; Lead; Lesion; Libraries; Lymphocyte; Mammalian Cell; Mass Spectrum Analysis; Molecular Biology; Mus; Operative Surgical Procedures; Pathogenesis; Pathogenicity; Patient Care; Patients; Pituitary Gland; Pituitary Gland Adenoma; Pituitary Hormones; Placental Lactogen; Procedures; Proteins; Proteomics; Protocols documentation; Recombinant Proteins; Reporting; SJL Mouse; Sampling; Sella Turcica Neoplasm; Serologic tests; Serum; Signs and Symptoms; Symptoms; Testing; Thyroid Gland; Translating; Two-Dimensional Gel Electrophoresis; adenoma; base; cDNA Expression; design; human disease; mouse model; public health relevance; tool; translation assay; two-dimensional

DESCRIPTION (provided by applicant): Masses of the pituitary gland (sellar masses) are common, representing approximately 15% of all intracranial masses. About half of the sellar masses are caused by a hormone-secreting adenoma and more easily diagnosed because of classical signs and symptoms. The remaining sellar masses, however, do not secrete any hormone (32% are non-functioning pituitary adenomas, and 10% are non-adenomatous lesions), and are therefore more difficult to differentiate. Often their diagnosis is established only after transsphenoidal surgery and pathological examination. Among the group of non-functioning pituitary masses, autoimmune (lymphocytic) hypophysitis is the only disease that has an immune pathogenesis. The pituitary autoantigens targeted in hypophysitis remain unknown. If discovered, they can lead to the development of a serum test useful to distinguish hypophysitis from the other non-functioning pituitary masses. This distinction is critical for patient care: in fact, hypophysitis can be usually managed medically, whereas the other conditions typically require surgery. The proposal reports our human and mouse studies aimed to discover the pituitary autoantigens and therefore develop an antibody-based serologic test for the differential diagnosis of non-functioning pituitary masses. PUBLIC HEALTH RELEVANCE: Pituitary masses are common. Because most of them do not secrete any pituitary hormone, they can often be diagnosed only after surgery and pathological examination. Autoimmune hypophysitis is the only disease entity among the non-functioning pituitary masses that has an immune pathogenesis. This grant is designed to develop an antibody- based serologic test to aid in the differential diagnosis of these common conditions. Such test could avoid unnecessary pituitary surgeries to patients with autoimmune hypophysitis.

描述（由申请人提供）：垂体肿块（鞍区肿块）很常见，约占所有颅内肿块的 15%。大约一半的鞍区肿块是由激素分泌腺瘤引起的，并且由于典型的体征和症状而更容易诊断。然而，其余的鞍区肿块不分泌任何激素（32％是无功能垂体腺瘤，10％是非腺瘤性病变），因此更难以区分。通常只有在经蝶手术和病理检查后才能确诊。在无功能垂体肿块中，自身免疫性（淋巴细胞性）垂体炎是唯一具有免疫发病机制的疾病。垂体炎靶向的垂体自身抗原仍然未知。如果被发现，它们可以导致血清测试的发展，有助于区分垂体炎和其他无功能的垂体肿块。这种区别对于患者护理至关重要：事实上，垂体炎通常可以通过药物治疗，而其他病症通常需要手术治疗。该提案报告了我们的人类和小鼠研究，旨在发现垂体自身抗原，从而开发一种基于抗体的血清学测试，用于鉴别诊断无功能的垂体肿块。公众健康相关性：垂体肿块很常见。由于大多不分泌任何垂体激素，往往需要手术和病理检查才能确诊。自身免疫性垂体炎是无功能垂体肿块中唯一具有免疫发病机制的疾病实体。这笔赠款旨在开发基于抗体的血清学测试，以帮助对这些常见病症进行鉴别诊断。这样的测试可以避免对自身免疫性垂体炎患者进行不必要的垂体手术。

项目成果

Preparation of mouse pituitary immunogen for the induction of experimental autoimmune hypophysitis.

• DOI: 10.3791/2181
• 发表时间: 2010-12
• 期刊: Journal of visualized experiments : JoVE
• 作者: Shey-Cherng Tzou;Melissa A. Landek-Salgado;Hiroaki Kimura;P. Caturegli

Pituitary antibodies in women with Hashimoto's thyroiditis: prevalence in diagnostic and prediagnostic sera.

桥本甲状腺炎女性的垂体抗体：诊断和诊断前血清中的患病率。

• DOI: 10.1089/thy.2011.0373
• 发表时间: 2012
• 期刊: Thyroid : official journal of the American Thyroid Association
• 作者: Guaraldi,Federica;Landek-Salgado,MelissaA;Hutfless,Susan;Samoni,Francesca;Talor,MonicaV;Matos,PeterG;Salvatori,Roberto;Rose,NoelR;Caturegli,Patrizio
• 通讯作者: Caturegli,Patrizio

Pituitary and systemic autoimmunity in a case of intrasellar germinoma.

• DOI: 10.1007/s11102-009-0187-x
• 发表时间: 2011-12
• 期刊: PITUITARY
• 影响因子: 3.8
• 作者: Gutenberg, Angelika;Bell, Jennifer J.;Lupi, Isabella;Tzou, Shey-Cherng;Landek-Salgado, Melissa A.;Kimura, Hiroaki;Su, Jack;Karaviti, Lefkothea P.;Salvatori, Roberto;Caturegli, Patrizio
• 通讯作者: Caturegli, Patrizio

Necrotizing infundibulo-hypophysitis: an entity too rare to be true?

• DOI: 10.1007/s11102-011-0307-2
• 发表时间: 2012-06
• 期刊: PITUITARY
• 影响因子: 3.8
• 作者: Gutenberg, Angelika;Caturegli, Patrizio;Metz, Imke;Martinez, Ramon;Mohr, Alexander;Brueck, Wolfgang;Rohde, Veit
• 通讯作者: Rohde, Veit

Induction of experimental autoimmune hypophysitis in SJL mice.

SJL 小鼠实验性自身免疫性垂体炎的诱导。

• DOI: 10.3791/2182
• 发表时间: 2010
• 期刊: Journal of visualized experiments : JoVE
• 作者: Landek-Salgado,MelissaA;Tzou,Shey-Cherng;Kimura,Hiroaki;Caturegli,Patrizio
• 通讯作者: Caturegli,Patrizio