CORE D - MUSC STEM CELL CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 细胞和分子工程核心(核心D)的目标是在以下方面为Cobre项目的研究人员提供资源和专业知识：1)向Cobre项目的研究人员提供从各种来源分离的细胞和组织；2)提供培养细胞和组织的资源和技术；3)产生并引入细胞和组织的质粒和病毒载体，以促进体外和体内的功能获得和功能丧失分析；4)通过通过抑制双链小干扰RNA(SiRNA)的特异性靶向抑制内源基因表达来执行遗传操作，并提供优化的方案；5)提供形态、免疫学以及基因和蛋白质分析方面的资源和专业知识，以检查细胞和组织分化并验证细胞表型；6)在开发和执行目标细胞群体的流式细胞仪分离和分析方面提供专业知识；以及7)指导Cobre学员在各自的实验室中使用这些技术来开发他们的独立研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the Cell and Molecular Engineering Core (Core D) is to provide resources and expertise to COBRE project investigators in the following areas: 1) provide cells and tissue isolated from various sources to COBRE investigators; 2) provides resources and techniques for growing cells and tissues in culture; 3) generate and introduce into cells and tissues plasmid and viral vectors to facilitate both gain-of-function and loss-of-function analyses in vitro and in vivo; 4) perform genetic manipulations by inhibition of endogenous gene expression using specific targeting by inhibitory double-stranded small interfering RNAs (siRNA) and offer optimized protocols; 5) provide resources and expertise for morphological, immunological, and gene and protein analyses to examine cell and tissue differentiation and verify cell phenotype; 6) provide expertise in developing and executing flow cytometric isolation and analyses of target cell populations; and 7) mentor the COBRE trainees in utilizing these techniques in their individual labs for development of their independent research programs.