CORE D - MUSC STEM CELL CORE

CORE D - 肌肉干细胞核心

• 批准号: 8360195
• 负责人: Richard P Visconti
• 金额: $ 22.99万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360195
• 关键词: Area; Biocompatible Materials; Cell Differentiation process; Cells; Centers of Research Excellence; Development; Engineering; Funding; Gene Expression; Gene Proteins; Goals; Grant; In Vitro; Individual; Mentors; Molecular; National Center for Research Resources; Phenotype; Plasmid Cloning Vector; Population; Principal Investigator; Protocols documentation; Research; Research Infrastructure; Research Personnel; Resources; Small Interfering RNA; Source; Techniques; Tissue Differentiation; Tissues; United States National Institutes of Health; Viral Vector; cost; gain of function; genetic manipulation; in vivo; loss of function; programs; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the Cell and Molecular Engineering Core (Core D) is to provide resources and expertise to COBRE project investigators in the following areas: 1) provide cells and tissue isolated from various sources to COBRE investigators; 2) provides resources and techniques for growing cells and tissues in culture; 3) generate and introduce into cells and tissues plasmid and viral vectors to facilitate both gain-of-function and loss-of-function analyses in vitro and in vivo; 4) perform genetic manipulations by inhibition of endogenous gene expression using specific targeting by inhibitory double-stranded small interfering RNAs (siRNA) and offer optimized protocols; 5) provide resources and expertise for morphological, immunological, and gene and protein analyses to examine cell and tissue differentiation and verify cell phenotype; 6) provide expertise in developing and executing flow cytometric isolation and analyses of target cell populations; and 7) mentor the COBRE trainees in utilizing these techniques in their individual labs for development of their independent research programs.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 细胞和分子工程核心（核心D）的目标是在以下领域为COBRE项目研究人员提供资源和专业知识：1）为COBRE研究人员提供从各种来源分离的细胞和组织; 2）提供培养细胞和组织的资源和技术; 3）产生质粒和病毒载体并将其引入细胞和组织中，以促进体外和体内的功能获得和功能丧失分析; 4）通过使用抑制性双链小干扰RNA（siRNA）的特异性靶向抑制内源基因表达来进行遗传操作，并提供优化的方案; 5）为形态学、免疫学以及基因和蛋白质分析提供资源和专业知识，以检查细胞和组织分化并验证细胞表型; 6）提供开发和执行流式细胞术分离和分析靶细胞群的专业知识; 7）指导COBRE学员在其各自的实验室中利用这些技术开发其独立的研究计划。