CORE D - MUSC STEM CELL CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞和分子工程核心（核心D）的目标是在以下领域为COBRE项目研究人员提供资源和专业知识：1）向COBRE研究人员提供从各种来源分离的细胞和组织; 2）提供培养细胞和组织的资源和技术; 3）产生质粒和病毒载体并将其引入细胞和组织中，以促进体外和体内的功能获得和功能丧失分析; 4）通过使用抑制性双链小干扰RNA（siRNA）的特异性靶向抑制内源基因表达来进行遗传操作，并提供优化的方案; 5）提供资源和专门知识，用于形态学，免疫学，以及基因和蛋白质分析，以检查细胞和组织分化并验证细胞表型; 6）提供开发和执行流式细胞术分离和分析靶细胞群的专业知识;指导COBRE学员在各自的实验室中使用这些技术，以发展他们的独立研究计划。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Cell and Molecular Engineering Core (Core D) is to provide resources and expertise to COBRE project investigators in the following areas: 1) proivde cells and tissue isolated from various sources to COBRE investigators; 2) provides resources and techniques for growing cells and tissues in culture; 3) generate and introduce into cells and tissues plasmid and viral vectors to facilitate both gain-of-function and loss-of-function analyses in vitro and in vivo; 4) perform genetic manipulations by inhibition of endogenous gene expression using specific targeting by inhibitory double-stranded small interfering RNAs (siRNA) and offer optimized protocols; 5) provide resources and expertise for morphological, immunological, and gene and protein analyses to examine cell and tissue differentiation and verify cell phenotype; 6) provide expertise in developing and executing flow cytometric isolation and analyses of target cell populations; and 7) mentor the COBRE trainees in uilizing these techniques in their individual labs for development of their independent research programs.