Synergistic effect of HIV virions and bacterial LPS on memory B cell apoptosis
HIV病毒体和细菌LPS对记忆B细胞凋亡的协同作用
基本信息
- 批准号:8210255
- 负责人:
- 金额:$ 31.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-01 至 2012-08-31
- 项目状态:已结题
- 来源:
- 关键词:AntibodiesAntibody FormationAntigensApoptosisApoptoticB-Cell ActivationB-Lymphocyte SubsetsB-LymphocytesBacterial DNABlood CellsCD4 Positive T LymphocytesCell Culture TechniquesCell DeathCellsCessation of lifeCharacteristicsChronicCross-Sectional StudiesDataDiseaseDisease ProgressionEnterocytesExposure toFlow CytometryFunctional disorderGoalsHIVHIV InfectionsHelper-Inducer T-LymphocyteHepatitis B VaccinesHepatitis B VirusHighly Active Antiretroviral TherapyHomeostasisHumoral ImmunitiesImmuneImmune responseImmunologicsIn VitroLigandsLipopolysaccharidesMeasuresMediatingMediator of activation proteinMemoryMemory B-LymphocyteModelingMolecularMononuclearOpportunistic InfectionsPathogenesisPathway interactionsPeripheralPlasmaPlayPolyvalent pneumococcal vaccineRNAReceptors, Antigen, B-CellRoleSorting - Cell MovementStreptococcus pneumoniaeT-LymphocyteTLR4 geneTestingVaccinationVaccinesViremiaVirioncohortimmune activationimprovedin vivoindexingmicrobialneutralizing antibodyreconstitutionresponsestemtherapy design
项目摘要
DESCRIPTION (provided by applicant): The gut mucosal barrier is disrupted in HIV disease, resulting in increased systemic exposure to microbial products such as Lipopolysaccharide (LPS) and bacterial DNA. Perturbations in the gut barrier in HIV disease may stem from deletion of CD4+ T cells, loss of Th17 helper cells, disruption of enterocyte homeostasis and diminished humoral immunity. The consequences of a permeable gut barrier in HIV infection are not fully understood, although there is evidence that exposure to microbial products could contribute to chronic immune activation in HIV disease and may also play a role in limiting CD4+ T cell reconstitution during highly active antiretroviral therapy (HAART). Here, we consider the possibility that microbial translocation also could play an important role in B cell perturbations that are characteristic of HIV infection. These perturbations include polyclonal B cell activation, B cell dysfunction and depletion of the memory B cell subset. Memory B cell dysfunction and depletion are the focus of our proposed studies as our preliminary data demonstrate that the TLR4 ligand LPS, and HIV virions cooperate to induce memory B cell death in peripheral blood cell cultures in vitro, enhanced memory B cell apoptosis ex vivo and plasma levels of LPS in vivo, and decreased level of recall antibody EndoCab (neutralizing antibody against LPS) in vivo from HIV+ donors. Therefore, we hypothesize that heightened microbial translocation and HIV replication collaborate to drive peripheral memory B depletion. We hypothesize that certain immune signatures of B cell subsets will predict reduced antibody responsiveness to vaccination (especially for T cell independent recall antigens) in HIV infection. We propose to explore the mechanisms responsible for TLR/HIV-mediated memory B cell death and to investigate in vivo correlates of these findings including identifying the predictors for reduced vaccine (both T cell dependent and independent antigens) responsiveness in HIV infection. By determining the mechanisms of B cell depletion and perturbations in HIV disease, we may be better able to design interventions that will potentially improve immune responses to vaccines, reduce selected opportunistic infections (e.g. pneumococcus) and perhaps slow disease progression by restoring the immunologic barrier that protects against microbial translocation.
PUBLIC HEALTH RELEVANCE: The purpose of the present study is to explore the mechanisms of peripheral memory B cell loss and reduced vaccine responsiveness in HIV infection. The goals of the present study are better to design interventions that will potentially improve immune responses to vaccines, reduce selected opportunistic infections (e.g. pneumococcus) and perhaps slow disease progression by restoring the immunologic barrier that protects against microbial translocation.
描述(由申请人提供):在 HIV 疾病中,肠粘膜屏障被破坏,导致全身暴露于脂多糖 (LPS) 和细菌 DNA 等微生物产物的增加。 HIV 疾病中肠道屏障的扰动可能源于 CD4+ T 细胞的缺失、Th17 辅助细胞的丧失、肠上皮细胞稳态的破坏和体液免疫的减弱。尽管有证据表明,接触微生物产品可能会导致 HIV 疾病的慢性免疫激活,并且还可能在高效抗逆转录病毒治疗 (HAART) 期间限制 CD4+ T 细胞重建,但可渗透性肠道屏障对 HIV 感染的影响尚不完全清楚。在这里,我们考虑微生物易位也可能在 HIV 感染特征的 B 细胞扰动中发挥重要作用的可能性。这些扰动包括多克隆 B 细胞激活、B 细胞功能障碍和记忆 B 细胞亚群的耗竭。记忆 B 细胞功能障碍和耗竭是我们拟议研究的重点,因为我们的初步数据表明,TLR4 配体 LPS 和 HIV 病毒体协同诱导体外外周血细胞培养物中的记忆 B 细胞死亡,增强离体记忆 B 细胞凋亡和体内 LPS 血浆水平,并降低记忆抗体 EndoCab(针对 LPS 的中和抗体)水平 来自 HIV+ 捐赠者的体内。因此,我们假设微生物易位增强和 HIV 复制共同导致外周记忆 B 耗竭。我们假设 B 细胞亚群的某些免疫特征将预测 HIV 感染中抗体对疫苗接种的反应性降低(特别是 T 细胞独立回忆抗原)。我们建议探索 TLR/HIV 介导的记忆 B 细胞死亡的机制,并研究这些发现的体内相关性,包括确定 HIV 感染中疫苗(T 细胞依赖性和独立抗原)反应性降低的预测因素。通过确定 HIV 疾病中 B 细胞耗竭和扰动的机制,我们也许能够更好地设计干预措施,从而改善对疫苗的免疫反应,减少选定的机会性感染(例如肺炎球菌),并可能通过恢复防止微生物易位的免疫屏障来减缓疾病进展。
公共健康相关性:本研究的目的是探讨 HIV 感染中外周记忆 B 细胞丢失和疫苗反应降低的机制。本研究的目标是更好地设计干预措施,潜在地改善对疫苗的免疫反应,减少选定的机会性感染(例如肺炎球菌),并可能通过恢复防止微生物易位的免疫屏障来减缓疾病进展。
项目成果
期刊论文数量(0)
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Wei Jiang其他文献
General Solution and Observability of Singular Differential Systems with Delay
时滞奇异微分系统的一般解与可观测性
- DOI:
10.1155/2013/512465 - 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
Wei Jiang - 通讯作者:
Wei Jiang
Wei Jiang的其他文献
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{{ truncateString('Wei Jiang', 18)}}的其他基金
Investigate Host Gene Isoforms Contributing to HIV Persistence in Cocaine Users
研究导致可卡因吸食者中艾滋病毒持续存在的宿主基因亚型
- 批准号:
10788990 - 财政年份:2023
- 资助金额:
$ 31.4万 - 项目类别:
Investigate the mechanism of autoreactive B cell-mediated immunological failure despite virologic suppression in HIV-infected individuals on antiretroviral therapy
研究尽管接受抗逆转录病毒治疗的 HIV 感染者出现病毒学抑制,但自身反应性 B 细胞介导的免疫失败的机制
- 批准号:
10595555 - 财政年份:2022
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$ 31.4万 - 项目类别:
Investigate the mechanism of autoreactive B cell-mediated immunological failure despite virologic suppression in HIV-infected individuals on antiretroviral therapy
研究尽管接受抗逆转录病毒治疗的 HIV 感染者出现病毒学抑制,但自身反应性 B 细胞介导的免疫失败的机制
- 批准号:
10368232 - 财政年份:2022
- 资助金额:
$ 31.4万 - 项目类别:
Investigate B cell perturbations and immune reconstitution failure in response to antiretroviral therapy in HIV-infected cocaine users
调查感染 HIV 的可卡因使用者抗逆转录病毒治疗导致的 B 细胞扰动和免疫重建失败
- 批准号:
10547870 - 财政年份:2022
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$ 31.4万 - 项目类别:
Investigate B cell perturbations and immune reconstitution failure in response to antiretroviral therapy in HIV-infected cocaine users
调查感染 HIV 的可卡因使用者抗逆转录病毒治疗导致的 B 细胞扰动和免疫重建失败
- 批准号:
10677040 - 财政年份:2022
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On the pathogenic role of anti-CD4 antibody in poor CD4+ T cell recovery after antiretroviral therapy in HIV disease
抗 CD4 抗体在 HIV 疾病抗逆转录病毒治疗后 CD4 T 细胞恢复不良中的致病作用
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9921289 - 财政年份:2017
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$ 31.4万 - 项目类别:
Synergistic effect of HIV virions and bacterial LPS on memory B cell apoptosis
HIV病毒体和细菌LPS对记忆B细胞凋亡的协同作用
- 批准号:
8534019 - 财政年份:2011
- 资助金额:
$ 31.4万 - 项目类别:
Synergistic effect of HIV virions and bacterial LPS on memory B cell apoptosis
HIV病毒体和细菌LPS对记忆B细胞凋亡的协同作用
- 批准号:
8321994 - 财政年份:2011
- 资助金额:
$ 31.4万 - 项目类别:
Synergistic effect of HIV virions and bacterial LPS on memory B cell apoptosis
HIV病毒体和细菌LPS对记忆B细胞凋亡的协同作用
- 批准号:
8719000 - 财政年份:2011
- 资助金额:
$ 31.4万 - 项目类别:
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