Morphine Actions on the Immune System

描述(由申请人提供)：这是我的K02独立科学家奖的续签申请，这将使我能够继续在西顿霍尔大学(SHU)获得最长的保护时间，用于我的NIDA资助的项目和指导研究型学生和初级科学家。在当前的K02资助期内，我一直致力于并成功续签了我长期获得的R01资助，题为“吗啡对免疫系统的作用”，并扩大了我的研究项目，如获得R21资助“阿片剂对HIV-1诱导的CMS炎症的影响”和R03资助“吗啡对HIV-1神经侵袭和小鼠脑变化的影响”(与David J.Volsky博士共同PI)，以研究药物滥用和HIV感染之间的相互作用。通过我的NSF重大研究仪器拨款，我在SHU实施了一个共焦设施，并在实时RT-PCR、基于荧光的生物检测和微阵列技术方面获得了丰富的经验。我曾指导研究生和初级研究人员，建立了负责任的研究行为(RCR)持续培训计划，并创建了一次为期一学期的分子生物科学会议，使研究生和本科生能够进行科学互动。此外，我还帮助建立了神经免疫药理学研究所(I-NIP)，其使命之一是将板凳工作翻译给社区。通过这次K02资助的续期，我将：1)研究我的R01 DA007058-17-22(PI)，并扩展我在R21 DA019836-01-02(PI)和R03 DA022169-01-02(Co-PI)奖项中提出的研究，分别开发一个新的R01项目；2)获得使用定量荧光显微镜、体视学程序和MicroBrightfield NeuroLeucida系统的经验，以便将我的体内研究提高到单细胞水平；3)获得动物行为测试经验，以确定在艾滋病毒感染存在的情况下滥用药物的影响；4)通过与临床科学家合作，完成I-NIP的使命，将基础科学带到社区；以及5)继续指导研究型学生、初级科学家和代表性不足的学生，强调RCR。我的研究项目和我在I-NIP中以研究为导向的培训计划是SHU的宝贵资产，在这份K02续签申请中提出的职业发展和研究计划得到了西顿霍尔大学艺术与科学学院的大力支持。

DESCRIPTION (provided by applicant): This is a renewal application for my K02 Independent Scientist Award that will enable me to continuously obtain maximum protected time at Seton Hall University (SHU) for working on my NIDA funded projects and mentoring research students and junior scientists. During the current K02 funding period, I have worked on and successfully renewed my long-standing R01 grant entitled, "Morphine Actions on the Immune System", and have expanded my research projects as evidenced by receiving an R21 grant, "Opiate's Effects on HIV- 1 Induced Inflammation in the CMS", and an R03 grant, "Effects of Morphine on HIV-1 Neuroinvasion and Brain Changes in Mice" (Co-Pi with Dr. David J. Volsky), to study the interactions between drug abuse and HIV infection. I have implemented a confocal facility at SHU via my NSF Major Research Instrumentation grant, and have gained extensive experience in real time RT-PCR, fluorescence-based biological assays, and microarray technology. I have mentored research students and junior researchers, established an ongoing training program in the responsible conduct of research (RCR), and created a semester-wide Molecular Bioscience Meeting to enable graduate and undergraduate students to interact scientifically. In addition, I have helped establish the Institute of Neurolmmune Pharmacology (I-NIP), one of its missions is to translate bench work to the community. With this K02 grant renewal, I will: 1) Work on my R01 DA007058-17-22 (PI), and extend the studies proposed in my R21 DA019836-01-02 (PI) and R03 DA022169-01-02 (Co-Pi) awards to develop a new R01 project, respectively; 2) Gain experience in using quantitative fluorescence microscopy, stereological procedures, and MicroBrightfield NeuroLeucida system in order to bring my in vivo research to the level of a single cell; 3) Gain experience in animal behavioral testing to determine the effects of drug abuse in the presence of HIV infection; 4) Bring basic science to the community by collaborating with clinical scientists and fulfilling the missions of the I-NIP; and 5) Continue to mentor research students, junior scientists, and under-represented students, emphasizing the RCR. My research projects and my initiatives in research-oriented training that are housed in the I-NIP are valuable assets to SHU, and the career development and research plans as proposed in this K02 renewal application have received tremendous support from the College of Arts and Sciences at Seton Hall University.