Morphine Actions on the Immune System

吗啡对免疫系统的作用

• 批准号: 8033231
• 负责人: SULIE L. CHANG
• 金额: $ 12.02万
• 依托单位: SETON HALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-05 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8033231
• 关键词: Adhesions; Adrenal Glands; Affect; Affinity; Animals; Anti-Inflammatory Agents; Arts; Attenuated; Award; Bacterial Infections; Basic Science; Biological Assay; Blood Coagulation Factor; Blood capillaries; Brain; Canis familiaris; Cell Nucleus; Cell model; Cells; Characteristics; Chemosensitization; Chronic; Clinical; Communities; Corticosterone; Data; Disease; Disseminated Intravascular Coagulation; Drug abuse; Endorphins; Endothelial Cells; Endotoxins; Exposure to; Fibrinopeptide A; Fluorescence; Fluorescence Microscopy; Funding; G-Protein-Coupled Receptors; GTP-Binding Proteins; Grant; HIV Infections; HIV-1; Housing; Hypothalamic structure; Immune; Immune response; Immune system; In Vitro; Independent Scientist Award; Infection; Inflammation; Inflammatory; Inflammatory Response; Institutes; Interleukin-1; Interleukin-6; Interleukins; Kidney; Leukocytes; Ligand Binding; Lipopolysaccharides; MDCK cell; Mediating; Mentors; Mesentery; Messenger RNA; Microarray Analysis; Mission; Molecular; Morphine; Mus; NF-kappa B; National Institute of Drug Abuse; Nervous system structure; Neurons; Neurosecretory Systems; Opiates; Opioid; Opioid Peptide; Opioid Receptor; Pathway interactions; Pharmacology; Pituitary Gland; Plasma; Procedures; Process; Production; Rattus; Reporting; Research; Research Design; Research Personnel; Research Project Grants; Reverse Transcriptase Polymerase Chain Reaction; Science; Scientist; Shock; Signal Transduction Pathway; Students; System; TNF gene; Testing; Time; Training; Training Programs; Translating; Transmembrane Domain; Tumor Necrosis Factor-alpha; Universities; Work; antithrombin III-protease complex; base; behavior test; capillary; career development; college; cytokine; drug of abuse; endogenous opioids; experience; hypothalamic-pituitary-adrenal axis; in vivo; instrumentation; meetings; mu opioid receptors; paraventricular nucleus; pressure; protein expression; receptor; receptor expression; research and development; response; responsible research conduct; venule

DESCRIPTION (provided by applicant): This is a renewal application for my K02 Independent Scientist Award that will enable me to continuously obtain maximum protected time at Seton Hall University (SHU) for working on my NIDA funded projects and mentoring research students and junior scientists. During the current K02 funding period, I have worked on and successfully renewed my long-standing R01 grant entitled, "Morphine Actions on the Immune System", and have expanded my research projects as evidenced by receiving an R21 grant, "Opiate's Effects on HIV- 1 Induced Inflammation in the CMS", and an R03 grant, "Effects of Morphine on HIV-1 Neuroinvasion and Brain Changes in Mice" (Co-Pi with Dr. David J. Volsky), to study the interactions between drug abuse and HIV infection. I have implemented a confocal facility at SHU via my NSF Major Research Instrumentation grant, and have gained extensive experience in real time RT-PCR, fluorescence-based biological assays, and microarray technology. I have mentored research students and junior researchers, established an ongoing training program in the responsible conduct of research (RCR), and created a semester-wide Molecular Bioscience Meeting to enable graduate and undergraduate students to interact scientifically. In addition, I have helped establish the Institute of Neurolmmune Pharmacology (I-NIP), one of its missions is to translate bench work to the community. With this K02 grant renewal, I will: 1) Work on my R01 DA007058-17-22 (PI), and extend the studies proposed in my R21 DA019836-01-02 (PI) and R03 DA022169-01-02 (Co-Pi) awards to develop a new R01 project, respectively; 2) Gain experience in using quantitative fluorescence microscopy, stereological procedures, and MicroBrightfield NeuroLeucida system in order to bring my in vivo research to the level of a single cell; 3) Gain experience in animal behavioral testing to determine the effects of drug abuse in the presence of HIV infection; 4) Bring basic science to the community by collaborating with clinical scientists and fulfilling the missions of the I-NIP; and 5) Continue to mentor research students, junior scientists, and under-represented students, emphasizing the RCR. My research projects and my initiatives in research-oriented training that are housed in the I-NIP are valuable assets to SHU, and the career development and research plans as proposed in this K02 renewal application have received tremendous support from the College of Arts and Sciences at Seton Hall University.

描述（由申请人提供）：这是我的K02独立科学家奖的续签申请，这将使我能够在西顿霍尔大学（SHU）持续获得最长的保护时间，用于我的NIDA资助项目和指导研究生和初级科学家。在目前的K02资助期间，我已经完成并成功续期了我长期的R01资助，题为“吗啡对免疫系统的作用”，并扩大了我的研究项目，如获得R21资助，“阿片类药物对HIV-1诱导炎症在CMS中的作用”，以及R03资助，“吗啡对HIV-1神经入侵和小鼠大脑变化的影响”（与David J. Volsky博士合作），研究药物滥用与HIV感染之间的相互作用。我通过我的NSF主要研究仪器拨款在SHU实施了一个共聚焦设备，并在实时RT-PCR，基于荧光的生物测定和微阵列技术方面获得了丰富的经验。我指导过研究生和初级研究人员，建立了一个负责任的研究行为（RCR）的持续培训计划，并创建了一个学期的分子生物科学会议，使研究生和本科生能够科学地互动。此外，我还帮助建立了神经免疫药理学研究所（I- nip），其任务之一是将实验室工作转化为社区。随着K02的续期，我将：1)继续我的R01 DA007058-17-22 （PI）的研究，并扩展我的R21 DA019836-01-02 （PI）和R03 DA022169-01-02 （Co-Pi）的研究，分别开发一个新的R01项目；2)获得使用定量荧光显微镜、立体程序和MicroBrightfield NeuroLeucida系统的经验，使我的体内研究达到单细胞水平；3)获得动物行为测试的经验，以确定HIV感染情况下药物滥用的影响；4)通过与临床科学家合作，完成I-NIP的使命，为社会带来基础科学；5)继续指导研究生、初级科学家和代表性不足的学生，强调RCR。我在I-NIP的研究项目和我在研究型培训方面的倡议是SHU的宝贵财富，并且在这次K02续签申请中提出的职业发展和研究计划得到了西顿霍尔大学文理学院的大力支持。