Acquisition of a 600 MHz NMR cryoprobe and 600 MHz spectrometer console upgrade

采购 600 MHz NMR 冷冻探头和 600 MHz 光谱仪控制台升级

• 批准号: 8050694
• 负责人: VALERIE COPIE
• 金额: $ 60万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2013-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8050694
• 关键词: Biochemical; Biochemistry; Biological; Biomedical Research; Cellular biology; Chemicals; Chemistry; Computer software; Data Collection; Development; Employment; Engineering; Frequencies; Funding; Laboratories; Microbial Biofilms; Molecular Biology; Montana; Neurosciences; Performance; Postdoctoral Fellow; Research; Research Infrastructure; Research Personnel; Sampling; Solubility; Solutions; Structural Chemistry; System; Systems Biology; Time; Training; United States National Institutes of Health; Universities; base; graduate student; instrument; instrumentation; metabolomics; programs; prospective; research study; structural biology

DESCRIPTION (provided by applicant): Support is requested to acquire a high-sensitivity 600 MHz triple resonance (1H, 13C, 15N) TCI cryoprobe and to upgrade an existing 600 MHz (1H Larmor frequency) NMR spectrometer console at Montana State University (MSU) to a more automated and high-throughput data collection platform (Bruker Avance IIITM). This instrumentation will be used by a diverse group of researchers from MSU Departments of Chemistry and Biochemistry, Veterinary Molecular Biology, Chemical and Biochemical Engineering, the Center for Biofilm Engineering, the Department of Cell Biology and Neuroscience, and the NIH Rocky Mountain Laboratory. Currently, none of the solution NMR spectrometers in the MSU Structural Biology and Chemistry NMR Facility are equipped with a cryoprobe, and the requested instrumentation and upgrade of the spectrometer console hardware will be the first of its kind on campus. The requested instrumentation upgrades will enhance sensitivity and maximize throughput of MSU 600 MHz solution NMR spectrometer. It will contribute significantly to the successful completion of challenging NMR-based structural biology projects and the expansion of NMR metabolomics research programs that are being developed on campus. The very substantially enhanced sensitivity achieved with the requested instrumentation will allow acquisition of experiments and studies on challenging biological samples, which are currently not possible because of limited sample solubility, limited sample stability, or limited instrument time availability. The console upgrade will allow automated shimming and performance optimization, and will provide much more efficient use of the recently purchased automatic sample loading system (Bruker SampleJetTM). The combination of the console upgrade and the automatic sample loading system will allow more effective 24/7 utilization of the 600 MHz NMR instrument. The cryoprobe and upgraded console will greatly facilitate the expansion of our existing NMR metabolomics capabilities (which include ChenomxTM and AMIXTM software for metabolite profiling analysis). Enhancement of NMR structural biology and metabolomics infrastructure is desired by a number of prospective users and supports the development of a new NIH-funded P20 Center for the Analysis of Cellular Mechanisms and Systems Biology. MSU's DRX 600 NMR instrument is used quite heavily, but the enhanced sensitivity and performance provided by the requested upgrades will allow this instrument to accomplish much more. The addition of the requested instrumentation will leverage significantly enhanced biomedical research capabilities for a broad group of researchers on the MSU-Bozeman campus. The requested instrumentation will also provide opportunities for new research directions in translational biomedical research, will support expanded systems biology approaches at MSU, and will contribute to the training and employment of numerous graduate students, post-doctoral fellows, and research staff. PUBLIC HEALTH RELEVANCE: We request support from the NIH to enhance the nuclear magnetic resonance (NMR) biomedical research capabilities of Montana State University. The requested instrumentation will enhance the research capabilities of a broad group of scientists involved in better understanding the molecular basis of iron homeostasis, host-pathogen interactions, viral infections, Alzheimer's, diabetes, AIDS, and other important human diseases.

描述（由申请人提供）：要求提供支持，以获得高灵敏度600 MHz三重共振（1H，13 C，15 N）TCI冷冻探针，并将蒙大拿州立大学（MSU）现有的600 MHz（1H Larmor频率）NMR光谱仪控制台升级为更自动化和高通量的数据收集平台（Bruker Avance IIITM）。该仪器将由来自MSU化学和生物化学，兽医分子生物学，化学和生物化学工程，生物膜工程中心，细胞生物学和神经科学系以及NIH落基山实验室的各种研究人员使用。目前，MSU结构生物学和化学NMR设施中的溶液NMR光谱仪都没有配备冷冻探针，所要求的仪器和光谱仪控制台硬件的升级将是校园内的第一个。 所要求的仪器升级将提高灵敏度，并最大限度地提高MSU 600 MHz溶液NMR光谱仪的吞吐量。它将为成功完成具有挑战性的基于NMR的结构生物学项目和扩展校园内正在开发的NMR代谢组学研究项目做出重大贡献。使用所要求的仪器实现的非常显著增强的灵敏度将允许对具有挑战性的生物样品进行实验和研究，由于有限的样品溶解度、有限的样品稳定性或有限的仪器时间可用性，这在目前是不可能的。控制台升级将允许自动填隙和性能优化，并将更有效地使用最近购买的自动进样系统（Bruker SampleJetTM）。控制台升级和自动样品装载系统相结合，将使600兆赫核磁共振仪得到更有效的24/7利用。 冷冻探针和升级后的控制台将极大地促进我们现有NMR代谢组学能力的扩展（包括用于代谢物分析的ChenomxTM和AMIXTM软件）。NMR结构生物学和代谢组学基础设施的增强是许多潜在用户所期望的，并支持NIH资助的新的P20细胞机制和系统生物学分析中心的发展。密歇根州立大学的DRX 600核磁共振仪使用相当频繁，但所要求的升级提供的增强灵敏度和性能将使该仪器完成更多。所要求的仪器的增加将利用显着增强的生物医学研究能力，为广大的研究人员在MSU博兹曼校园。所要求的仪器还将为转化生物医学研究的新研究方向提供机会，将支持MSU扩展的系统生物学方法，并将有助于培训和雇用众多研究生，博士后研究员和研究人员。 公共卫生相关性：我们请求国家卫生研究院的支持，以提高蒙大拿州立大学的核磁共振（NMR）生物医学研究能力。所要求的仪器将提高参与更好地理解铁稳态、宿主-病原体相互作用、病毒感染、阿尔茨海默氏症、糖尿病、艾滋病和其他重要人类疾病的分子基础的广大科学家的研究能力。

项目成果

Exposure of Methicillin-Resistant Staphylococcus aureus to Low Levels of the Antibacterial THAM-3ΦG Generates a Small Colony Drug-Resistant Phenotype.

将耐甲氧西林金黄色葡萄球菌暴露于低水平的抗菌 THAM-3μG 会产生小菌落耐药表型。

• DOI: 10.1038/s41598-018-28283-3
• 发表时间: 2018
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Weaver,AlanJ;Peters,TamiR;Tripet,Brian;VanVuren,Abigail;Rakesh;Lee,RichardE;Copié,Valérie;Teintze,Martin
• 通讯作者: Teintze,Martin

Metabolomic and elemental profiling of human tissue in kidney cancer.

• DOI: 10.1007/s11306-021-01779-2
• 发表时间: 2021-03-04
• 期刊: Metabolomics : Official journal of the Metabolomic Society
• 作者: Nizioł J;Copié V;Tripet BP;Nogueira LB;Nogueira KOPC;Ossoliński K;Arendowski A;Ruman T
• 通讯作者: Ruman T