Acquisition of a 600 MHz NMR cryoprobe and 600 MHz spectrometer console upgrade

采购 600 MHz NMR 冷冻探头和 600 MHz 光谱仪控制台升级

• 批准号: 8050694
• 负责人: VALERIE COPIE
• 金额: $ 60万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2013-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8050694
• 关键词: Biochemical; Biochemistry; Biological; Biomedical Research; Cellular biology; Chemicals; Chemistry; Computer software; Data Collection; Development; Employment; Engineering; Frequencies; Funding; Laboratories; Microbial Biofilms; Molecular Biology; Montana; Neurosciences; Performance; Postdoctoral Fellow; Research; Research Infrastructure; Research Personnel; Sampling; Solubility; Solutions; Structural Chemistry; System; Systems Biology; Time; Training; United States National Institutes of Health; Universities; base; graduate student; instrument; instrumentation; metabolomics; programs; prospective; research study; structural biology

DESCRIPTION (provided by applicant): Support is requested to acquire a high-sensitivity 600 MHz triple resonance (1H, 13C, 15N) TCI cryoprobe and to upgrade an existing 600 MHz (1H Larmor frequency) NMR spectrometer console at Montana State University (MSU) to a more automated and high-throughput data collection platform (Bruker Avance IIITM). This instrumentation will be used by a diverse group of researchers from MSU Departments of Chemistry and Biochemistry, Veterinary Molecular Biology, Chemical and Biochemical Engineering, the Center for Biofilm Engineering, the Department of Cell Biology and Neuroscience, and the NIH Rocky Mountain Laboratory. Currently, none of the solution NMR spectrometers in the MSU Structural Biology and Chemistry NMR Facility are equipped with a cryoprobe, and the requested instrumentation and upgrade of the spectrometer console hardware will be the first of its kind on campus. The requested instrumentation upgrades will enhance sensitivity and maximize throughput of MSU 600 MHz solution NMR spectrometer. It will contribute significantly to the successful completion of challenging NMR-based structural biology projects and the expansion of NMR metabolomics research programs that are being developed on campus. The very substantially enhanced sensitivity achieved with the requested instrumentation will allow acquisition of experiments and studies on challenging biological samples, which are currently not possible because of limited sample solubility, limited sample stability, or limited instrument time availability. The console upgrade will allow automated shimming and performance optimization, and will provide much more efficient use of the recently purchased automatic sample loading system (Bruker SampleJetTM). The combination of the console upgrade and the automatic sample loading system will allow more effective 24/7 utilization of the 600 MHz NMR instrument. The cryoprobe and upgraded console will greatly facilitate the expansion of our existing NMR metabolomics capabilities (which include ChenomxTM and AMIXTM software for metabolite profiling analysis). Enhancement of NMR structural biology and metabolomics infrastructure is desired by a number of prospective users and supports the development of a new NIH-funded P20 Center for the Analysis of Cellular Mechanisms and Systems Biology. MSU's DRX 600 NMR instrument is used quite heavily, but the enhanced sensitivity and performance provided by the requested upgrades will allow this instrument to accomplish much more. The addition of the requested instrumentation will leverage significantly enhanced biomedical research capabilities for a broad group of researchers on the MSU-Bozeman campus. The requested instrumentation will also provide opportunities for new research directions in translational biomedical research, will support expanded systems biology approaches at MSU, and will contribute to the training and employment of numerous graduate students, post-doctoral fellows, and research staff. PUBLIC HEALTH RELEVANCE: We request support from the NIH to enhance the nuclear magnetic resonance (NMR) biomedical research capabilities of Montana State University. The requested instrumentation will enhance the research capabilities of a broad group of scientists involved in better understanding the molecular basis of iron homeostasis, host-pathogen interactions, viral infections, Alzheimer's, diabetes, AIDS, and other important human diseases.

描述（由申请人提供）：要求获得高灵敏度600 MHz三共振（1H, 13C, 15N） TCI冷冻探针，并将蒙大拿州立大学（MSU）现有的600 MHz （1H Larmor频率）核磁共振光谱仪控制台升级为更自动化和高通量的数据收集平台（Bruker Avance IIITM）。该仪器将由密歇根州立大学化学和生物化学系、兽医分子生物学系、化学和生物化学工程系、生物膜工程中心、细胞生物学和神经科学系以及美国国立卫生研究院落基山实验室的不同研究人员使用。目前，密歇根州立大学结构生物和化学核磁共振设施的所有解决方案核磁共振光谱仪都没有配备冷冻探针，而所要求的仪器和光谱仪控制台硬件的升级将是校园内第一个此类设备。所要求的仪器升级将提高MSU 600 MHz溶液核磁共振光谱仪的灵敏度和最大吞吐量。它将为成功完成具有挑战性的核磁共振结构生物学项目和正在校园开发的核磁共振代谢组学研究项目的扩展做出重大贡献。由于样品溶解度有限、样品稳定性有限或仪器时间有限，目前无法对具有挑战性的生物样品进行实验和研究。控制台升级将允许自动摆振和性能优化，并将提供更有效地使用最近购买的自动样品装载系统（Bruker SampleJetTM）。控制台升级和自动样品装载系统的结合将允许更有效地利用600 MHz核磁共振仪器。冷冻探针和升级后的控制台将极大地促进我们现有的核磁共振代谢组学功能的扩展（其中包括用于代谢物分析的ChenomxTM和AMIXTM软件）。许多潜在用户希望增强核磁共振结构生物学和代谢组学基础设施，并支持美国国立卫生研究院资助的新的细胞机制和系统生物学分析P20中心的发展。MSU的DRX 600核磁共振仪器被大量使用，但要求升级提供的增强灵敏度和性能将使该仪器能够完成更多工作。所要求的仪器的增加将大大提高生物医学研究能力，为密歇根州立大学博兹曼校区的广泛研究人员提供支持。所要求的仪器还将为转化生物医学研究的新研究方向提供机会，将支持密歇根州立大学扩展的系统生物学方法，并将有助于培训和就业众多研究生，博士后研究员和研究人员。

项目成果

Exposure of Methicillin-Resistant Staphylococcus aureus to Low Levels of the Antibacterial THAM-3ΦG Generates a Small Colony Drug-Resistant Phenotype.

将耐甲氧西林金黄色葡萄球菌暴露于低水平的抗菌 THAM-3μG 会产生小菌落耐药表型。

• DOI: 10.1038/s41598-018-28283-3
• 发表时间: 2018
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Weaver,AlanJ;Peters,TamiR;Tripet,Brian;VanVuren,Abigail;Rakesh;Lee,RichardE;Copié,Valérie;Teintze,Martin
• 通讯作者: Teintze,Martin

Metabolomic and elemental profiling of human tissue in kidney cancer.

• DOI: 10.1007/s11306-021-01779-2
• 发表时间: 2021-03-04
• 期刊: Metabolomics : Official journal of the Metabolomic Society
• 作者: Nizioł J;Copié V;Tripet BP;Nogueira LB;Nogueira KOPC;Ossoliński K;Arendowski A;Ruman T
• 通讯作者: Ruman T