SHARED SOLUTION 600 MHZ NMR INSTRUMENT

申请资金用于购置一台液态14.1特斯拉（600兆赫质子频率）核磁共振波谱仪，供来自蒙大拿州立大学化学和生物化学、微生物学、兽医分子生物学和植物病理学系以及蒙大拿理工大学和蒙大拿大学的一批高产科学家使用。该仪器将扩大目前参与结构生物学研究的一大群MSU研究人员的NMR研究能力。大多数项目需要收集溶液中大蛋白质或肽-蛋白质复合物的高分辨率NMR数据。购买600 MHz NMR光谱仪也将释放当前500 MHz NMR光谱仪的NMR时间，使其更适用于参与生物活性化合物合成和表征的化学家。目前，MSU 500：MHz NMR光谱仪每天24小时运行，99%的时间被膜蛋白研究小组的成员使用，Copie博士的研究涉及蛋白质的结构-功能研究，使用最近开发的多核和多维溶液NMR方法。对500 MHz NMR时间不断增长的需求已经超过了可用的仪器时间。获得600 MHz光谱仪将实现两个目标：1）它将使结构生物学小组的成员能够利用600 MHz NMR仪器的更高灵敏度和分辨率来推进他们对蛋白质或肽-蛋白质复合物的结构-功能研究，以及2）。它将为广泛的有机化学研究提供更容易使用的500 MHz NMR光谱仪。有了新的仪器，下面列出的研究人员，沿着与一组几个二级研究人员，将能够将他们的研究扩展到更大的分子，并进行与生物系统中的结构和功能相关的竞争性研究。14.1 T光谱仪的可用性将使研究人员能够访问目前超额预订的11.7 T仪器。

Funds are requested for the acquisition of a liquid state 14.1 Tesla (600 MHz proton frequency) NMR spectrometer to be used by a diverse group of highly productive scientists from the Departments of Chemistry and Biochemistry, Microbiology, Veterinary Molecular Biology, and Plant Pathology at Montana State University, as well as Montana Tech, and the University of Montana. This instrument would expand the NMR-based research capabilities of a large group of MSU investigators currently involved in structural biology research. The majority of the projects require the collection of high resolution NMR data on large proteins, or on peptide-protein complexes, in solution. Acquisition of a 600 MHz NMR spectrometer would also free up NMR time on the current 500 MHz NMR spectrometer, making it more available to chemists involved in the synthesis and characterization of bioactive compounds. At present, the MSU 500:MHz NMR spectrometer operates 24 hours/day, and is used 99% of the time by members of the membrane protein research group, and by Dr. Copie's research which concerns the structure-function study of proteins, using recently developed multinuclear and multi-dimensional solution NMR methods. The growing demand for 500 MHz NMR time already exceeds the amount of instrument time available. Acquisition of a 600 MHz spectrometer would accomplish two goals: 1) It would enable members of the structural biology group to take advantage of the greater sensitivity and resolution of a 600 MHz NMR instrument to advance their structure-function studies on proteins, or on peptide- protein complexes, and 2). It would provide easier access to the 500 MHz NMR spectrometer for a wide range of organic chemistry research. With the new instrument, the investigators listed below, along with a group of several secondary investigators, will be in a position to expand their research to larger molecules, and to carry out competitive research related to structure and function in biological systems. The availability of the 14.1 T spectrometer will allow researchers to access the currently overbooked 11.7 T instrument.