SHARED SOLUTION 600 MHZ NMR INSTRUMENT

申请资金用于购买一台液态14.1特斯拉（600兆赫质子频率）核磁共振光谱仪，供蒙大拿州立大学、蒙大拿州理工大学和蒙大拿州大学化学与生物化学、微生物学、兽医分子生物学和植物病理学等部门的高效科学家使用。该仪器将扩大一大批目前参与结构生物学研究的密歇根州立大学研究人员基于核磁共振的研究能力。大多数项目需要在溶液中收集大蛋白质或肽-蛋白质复合物的高分辨率核磁共振数据。获得600 MHz核磁共振波谱仪也将解放目前500 MHz核磁共振波谱仪的核磁共振时间，使其更容易用于参与合成和表征生物活性化合物的化学家。目前，MSU 500:MHz核磁共振波谱仪全天24小时运行，99%的时间用于膜蛋白研究小组的成员，以及Copie博士的研究，该研究涉及蛋白质的结构-功能研究，使用最新开发的多核和多维溶液核磁共振方法。对500 MHz核磁共振时间的不断增长的需求已经超过了仪器可用的时间。获得600兆赫光谱仪将实现两个目标：1)它将使结构生物学组的成员能够利用600兆赫核磁共振仪器更高的灵敏度和分辨率来推进他们对蛋白质或肽-蛋白质复合物的结构-功能研究；2)。它将为广泛的有机化学研究提供更方便的500兆赫核磁共振光谱仪。有了新仪器，下面列出的研究人员，以及一组几名二级研究人员，将能够将他们的研究扩展到更大分子，并开展与生物系统结构和功能相关的竞争性研究。14.1 T光谱仪的可用性将使研究人员能够使用目前超额预订的11.7 T仪器。

Funds are requested for the acquisition of a liquid state 14.1 Tesla (600 MHz proton frequency) NMR spectrometer to be used by a diverse group of highly productive scientists from the Departments of Chemistry and Biochemistry, Microbiology, Veterinary Molecular Biology, and Plant Pathology at Montana State University, as well as Montana Tech, and the University of Montana. This instrument would expand the NMR-based research capabilities of a large group of MSU investigators currently involved in structural biology research. The majority of the projects require the collection of high resolution NMR data on large proteins, or on peptide-protein complexes, in solution. Acquisition of a 600 MHz NMR spectrometer would also free up NMR time on the current 500 MHz NMR spectrometer, making it more available to chemists involved in the synthesis and characterization of bioactive compounds. At present, the MSU 500:MHz NMR spectrometer operates 24 hours/day, and is used 99% of the time by members of the membrane protein research group, and by Dr. Copie's research which concerns the structure-function study of proteins, using recently developed multinuclear and multi-dimensional solution NMR methods. The growing demand for 500 MHz NMR time already exceeds the amount of instrument time available. Acquisition of a 600 MHz spectrometer would accomplish two goals: 1) It would enable members of the structural biology group to take advantage of the greater sensitivity and resolution of a 600 MHz NMR instrument to advance their structure-function studies on proteins, or on peptide- protein complexes, and 2). It would provide easier access to the 500 MHz NMR spectrometer for a wide range of organic chemistry research. With the new instrument, the investigators listed below, along with a group of several secondary investigators, will be in a position to expand their research to larger molecules, and to carry out competitive research related to structure and function in biological systems. The availability of the 14.1 T spectrometer will allow researchers to access the currently overbooked 11.7 T instrument.